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Title: Biosensors engineered from conditionally stable ligand-binding domains

Abstract

Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.

Inventors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1393435
Patent Number(s):
9,766,255
Application Number:
14/993,509
Assignee:
President and Fellows of Harvard College (Cambridge, MA); University of Washington (Seattle, WA)
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Jan 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Feng, Justin, Mandell, Daniel J., Baker, David, Fields, Stanley, Jester, Benjamin Ward, and Tinberg, Christine Elaine. Biosensors engineered from conditionally stable ligand-binding domains. United States: N. p., 2017. Web.
Church, George M., Feng, Justin, Mandell, Daniel J., Baker, David, Fields, Stanley, Jester, Benjamin Ward, & Tinberg, Christine Elaine. Biosensors engineered from conditionally stable ligand-binding domains. United States.
Church, George M., Feng, Justin, Mandell, Daniel J., Baker, David, Fields, Stanley, Jester, Benjamin Ward, and Tinberg, Christine Elaine. Tue . "Biosensors engineered from conditionally stable ligand-binding domains". United States. https://www.osti.gov/servlets/purl/1393435.
@article{osti_1393435,
title = {Biosensors engineered from conditionally stable ligand-binding domains},
author = {Church, George M. and Feng, Justin and Mandell, Daniel J. and Baker, David and Fields, Stanley and Jester, Benjamin Ward and Tinberg, Christine Elaine},
abstractNote = {Disclosed is a biosensor engineered to conditionally respond to the presence of specific small molecules, the biosensors including conditionally stable ligand-binding domains (LBDs) which respond to the presence of specific small molecules, wherein readout of binding is provided by reporter genes or transcription factors (TFs) fused to the LBDs.},
doi = {},
url = {https://www.osti.gov/biblio/1393435}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {9}
}

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Works referenced in this record:

Computational design of ligand-binding proteins with high affinity and selectivity
journal, September 2013


Destabilizing Domains Derived from the Human Estrogen Receptor
journal, February 2012


Screening for Enhanced Triacetic Acid Lactone Production by Recombinant Escherichia coli Expressing a Designed Triacetic Acid Lactone Reporter
journal, June 2013

  • Tang, Shuang-Yan; Qian, Shuai; Akinterinwa, Olubolaji
  • Journal of the American Chemical Society, Vol. 135, Issue 27, p. 10099-10103
  • https://doi.org/10.1021/ja402654z