Structural analysis of cell wall polysaccharides using PACE
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Institute
The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. Many techniques to characterize polysaccharide structure are complicated, requiring expensive equipment and specialized operators e.g. NMR, MALDI-MS. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al. 2002). Whilst the method here describes xylan analysis, it can be applied (by use of the appropriate glycosyl hydrolase) to any cell wall polysaccharide.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 1393097
- Resource Relation:
- Related Information: Book Title: Methods in Molecular Biology
- Country of Publication:
- United States
- Language:
- English
Polysaccharide Analysis Using Carbohydrate Gel Electrophoresis: A Method to Study Plant Cell Wall Polysaccharides and Polysaccharide Hydrolases
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journal | January 2002 |
Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls
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journal | July 2006 |
Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
|
journal | December 2007 |
Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass
|
journal | September 2010 |
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