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Title: Structural analysis of cell wall polysaccharides using PACE

Abstract

The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. Many techniques to characterize polysaccharide structure are complicated, requiring expensive equipment and specialized operators e.g. NMR, MALDI-MS. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al. 2002). Whilst the method here describes xylan analysis, it can be applied (by use of the appropriate glycosyl hydrolase) to any cell wall polysaccharide.

Authors:
 [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Institute
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1393097
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Book
Resource Relation:
Related Information: Book Title: Methods in Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mortimer, Jennifer C. Structural analysis of cell wall polysaccharides using PACE. United States: N. p., 2017. Web. doi:10.1007/978-1-4939-6722-3_16.
Mortimer, Jennifer C. Structural analysis of cell wall polysaccharides using PACE. United States. doi:10.1007/978-1-4939-6722-3_16.
Mortimer, Jennifer C. Sun . "Structural analysis of cell wall polysaccharides using PACE". United States. doi:10.1007/978-1-4939-6722-3_16. https://www.osti.gov/servlets/purl/1393097.
@article{osti_1393097,
title = {Structural analysis of cell wall polysaccharides using PACE},
author = {Mortimer, Jennifer C.},
abstractNote = {The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. Many techniques to characterize polysaccharide structure are complicated, requiring expensive equipment and specialized operators e.g. NMR, MALDI-MS. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al. 2002). Whilst the method here describes xylan analysis, it can be applied (by use of the appropriate glycosyl hydrolase) to any cell wall polysaccharide.},
doi = {10.1007/978-1-4939-6722-3_16},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {1}
}

Book:
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Works referenced in this record:

Polysaccharide Analysis Using Carbohydrate Gel Electrophoresis: A Method to Study Plant Cell Wall Polysaccharides and Polysaccharide Hydrolases
journal, January 2002

  • Goubet, Florence; Jackson, Peter; Deery, Michael J.
  • Analytical Biochemistry, Vol. 300, Issue 1
  • DOI: 10.1006/abio.2001.5444

Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls
journal, July 2006

  • Bauer, S.; Vasu, P.; Persson, S.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 30
  • DOI: 10.1073/pnas.0604632103

Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
journal, December 2007


Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass
journal, September 2010

  • Mortimer, J. C.; Miles, G. P.; Brown, D. M.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 40
  • DOI: 10.1073/pnas.1005456107