skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Abstract

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

Authors:
 [1];  [2];  [3];  [3];  [4];  [4];  [5];  [4];  [3];  [4];  [3];  [2];  [4]
  1. Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); The Australian National Univ., Acton, ACT (Australia)
  2. Univ. of California, Irvine, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. Univ. of California, Davis, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1393041
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Integrative Biology
Additional Journal Information:
Journal Volume: 8; Journal Issue: 4; Journal ID: ISSN 1757-9694
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., and Ronald, Pamela C. A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection. United States: N. p., 2015. Web. doi:10.1039/c5ib00232j.
Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., & Ronald, Pamela C. A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection. United States. doi:10.1039/c5ib00232j.
Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., and Ronald, Pamela C. Fri . "A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection". United States. doi:10.1039/c5ib00232j. https://www.osti.gov/servlets/purl/1393041.
@article{osti_1393041,
title = {A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection},
author = {Schwessinger, Benjamin and Li, Xiang and Ellinghaus, Thomas L. and Chan, Leanne Jade G. and Wei, Tong and Joe, Anna and Thomas, Nicholas and Pruitt, Rory and Adams, Paul D. and Chern, Maw Sheng and Petzold, Christopher J. and Liu, Chang C. and Ronald, Pamela C.},
abstractNote = {Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.},
doi = {10.1039/c5ib00232j},
journal = {Integrative Biology},
issn = {1757-9694},
number = 4,
volume = 8,
place = {United States},
year = {2015},
month = {11}
}

Works referenced in this record:

Recombinant expression of selectively sulfated proteins in Escherichia coli
journal, October 2006

  • Liu, Chang C.; Schultz, Peter G.
  • Nature Biotechnology, Vol. 24, Issue 11
  • DOI: 10.1038/nbt1254

Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code
journal, November 2009

  • Liu, Chang C.; Cellitti, Susan E.; Geierstanger, Bernhard H.
  • Nature Protocols, Vol. 4, Issue 12
  • DOI: 10.1038/nprot.2009.188

Toward a framework for sulfoproteomics: Synthesis and characterization of sulfotyrosine-containing peptides
journal, August 2007

  • Seibert, Christoph; Sakmar, Thomas P.
  • Biopolymers, Vol. 90, Issue 3
  • DOI: 10.1002/bip.20821

Mutagenesis and Evolution of Sulfated Antibodies Using an Expanded Genetic Code
journal, September 2009

  • Liu, Chang C.; Choe, Hyeryun; Farzan, Michael
  • Biochemistry, Vol. 48, Issue 37
  • DOI: 10.1021/bi9011429

Adding New Chemistries to the Genetic Code
journal, June 2010


The structural role of receptor tyrosine sulfation in chemokine recognition: Tyrosine sulfation of chemokine receptors
journal, February 2014

  • Ludeman, Justin P.; Stone, Martin J.
  • British Journal of Pharmacology, Vol. 171, Issue 5
  • DOI: 10.1111/bph.12455

Minimal model systems for β-sheet secondary structure in proteins
journal, January 1998


Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins
journal, June 2009


AAV-expressed eCD4-Ig provides durable protection from multiple SHIV challenges
journal, February 2015

  • Gardner, Matthew R.; Kattenhorn, Lisa M.; Kondur, Hema R.
  • Nature, Vol. 519, Issue 7541
  • DOI: 10.1038/nature14264

Tyrosine sulfation in a Gram-negative bacterium
journal, January 2012

  • Han, Sang-Wook; Lee, Sang-Won; Bahar, Ofir
  • Nature Communications, Vol. 3, Issue 1
  • DOI: 10.1038/ncomms2157

Crystal Structure of a Biosynthetic Sulfo-hirudin Complexed to Thrombin
journal, September 2007

  • Liu, Chang C.; Brustad, Eric; Liu, Wenshe
  • Journal of the American Chemical Society, Vol. 129, Issue 35
  • DOI: 10.1021/ja0735002

The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium
journal, July 2015

  • Pruitt, Rory N.; Schwessinger, Benjamin; Joe, Anna
  • Science Advances, Vol. 1, Issue 6
  • DOI: 10.1126/sciadv.1500245

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli
journal, February 2013

  • Chatterjee, Abhishek; Sun, Sophie B.; Furman, Jennifer L.
  • Biochemistry, Vol. 52, Issue 10
  • DOI: 10.1021/bi4000244

Structures of the CCR5 N Terminus and of a Tyrosine-Sulfated Antibody with HIV-1 gp120 and CD4
journal, September 2007


Genomically Recoded Organisms Expand Biological Functions
journal, October 2013

  • Lajoie, M. J.; Rovner, A. J.; Goodman, D. B.
  • Science, Vol. 342, Issue 6156, p. 357-360
  • DOI: 10.1126/science.1241459

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
journal, January 2010


Protein evolution with an expanded genetic code
journal, November 2008

  • Liu, C. C.; Mack, A. V.; Tsao, M. -L.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 46
  • DOI: 10.1073/pnas.0809543105

Protein sulfation analysis—A primer
journal, December 2006

  • Monigatti, Flavio; Hekking, Brian; Steen, Hanno
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1764, Issue 12
  • DOI: 10.1016/j.bbapap.2006.07.002

Using circular dichroism spectra to estimate protein secondary structure
journal, January 2007