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Title: A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Abstract

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

Authors:
 [1];  [2];  [3];  [3];  [4];  [4];  [5];  [4];  [3];  [4];  [3];  [2];  [4]
  1. Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); The Australian National Univ., Acton, ACT (Australia)
  2. Univ. of California, Irvine, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. Univ. of California, Davis, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1393041
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Integrative Biology
Additional Journal Information:
Journal Volume: 8; Journal Issue: 4; Journal ID: ISSN 1757-9694
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., and Ronald, Pamela C. A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection. United States: N. p., 2015. Web. doi:10.1039/c5ib00232j.
Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., & Ronald, Pamela C. A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection. United States. doi:10.1039/c5ib00232j.
Schwessinger, Benjamin, Li, Xiang, Ellinghaus, Thomas L., Chan, Leanne Jade G., Wei, Tong, Joe, Anna, Thomas, Nicholas, Pruitt, Rory, Adams, Paul D., Chern, Maw Sheng, Petzold, Christopher J., Liu, Chang C., and Ronald, Pamela C. Fri . "A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection". United States. doi:10.1039/c5ib00232j. https://www.osti.gov/servlets/purl/1393041.
@article{osti_1393041,
title = {A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection},
author = {Schwessinger, Benjamin and Li, Xiang and Ellinghaus, Thomas L. and Chan, Leanne Jade G. and Wei, Tong and Joe, Anna and Thomas, Nicholas and Pruitt, Rory and Adams, Paul D. and Chern, Maw Sheng and Petzold, Christopher J. and Liu, Chang C. and Ronald, Pamela C.},
abstractNote = {Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.},
doi = {10.1039/c5ib00232j},
journal = {Integrative Biology},
number = 4,
volume = 8,
place = {United States},
year = {Fri Nov 27 00:00:00 EST 2015},
month = {Fri Nov 27 00:00:00 EST 2015}
}

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Cited by: 5 works
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Works referenced in this record:

Genomically Recoded Organisms Expand Biological Functions
journal, October 2013

  • Lajoie, M. J.; Rovner, A. J.; Goodman, D. B.
  • Science, Vol. 342, Issue 6156, p. 357-360
  • DOI: 10.1126/science.1241459

Using circular dichroism spectra to estimate protein secondary structure
journal, January 2007