A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Schwessinger, Benjamin; Li, Xiang; Ellinghaus, Thomas L.; Chan, Leanne Jade G.; Wei, Tong; Joe, Anna; Thomas, Nicholas; Pruitt, Rory; Adams, Paul D.; Chern, Maw Sheng; Petzold, Christopher J.; Liu, Chang C.; Ronald, Pamela C.

doi:10.1039/c5ib00232j

Title: A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Journal Article · Fri Nov 27 00:00:00 EST 2015 · Integrative Biology

DOI:https://doi.org/10.1039/c5ib00232j· OSTI ID:1393041

Schwessinger, Benjamin ^[1]; Li, Xiang ^[2]; Ellinghaus, Thomas L. ^[3]; Chan, Leanne Jade G. ^[3]; Wei, Tong ^[4]; Joe, Anna ^[4]; Thomas, Nicholas ^[5]; Pruitt, Rory ^[4]; Adams, Paul D. ^[3]; Chern, Maw Sheng ^[4]; Petzold, Christopher J. ^[3]; Liu, Chang C. ^[2]; Ronald, Pamela C. ^[4]

Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); The Australian National Univ., Acton, ACT (Australia)
Univ. of California, Irvine, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Davis, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Davis, CA (United States)

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1393041

Journal Information:: Integrative Biology, Vol. 8, Issue 4; ISSN 1757-9694

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

References (19)

Recombinant expression of selectively sulfated proteins in Escherichia coli Liu, Chang C.; Schultz, Peter G. Nature Biotechnology, Vol. 24, Issue 11 https://doi.org/10.1038/nbt1254	journal	October 2006
Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code Liu, Chang C.; Cellitti, Susan E.; Geierstanger, Bernhard H. Nature Protocols, Vol. 4, Issue 12 https://doi.org/10.1038/nprot.2009.188	journal	November 2009
Toward a framework for sulfoproteomics: Synthesis and characterization of sulfotyrosine-containing peptides Seibert, Christoph; Sakmar, Thomas P. Biopolymers, Vol. 90, Issue 3 https://doi.org/10.1002/bip.20821	journal	August 2007
Mutagenesis and Evolution of Sulfated Antibodies Using an Expanded Genetic Code Liu, Chang C.; Choe, Hyeryun; Farzan, Michael Biochemistry, Vol. 48, Issue 37 https://doi.org/10.1021/bi9011429	journal	September 2009
Adding New Chemistries to the Genetic Code Liu, Chang C.; Schultz, Peter G. Annual Review of Biochemistry, Vol. 79, Issue 1, p. 413-444 https://doi.org/10.1146/annurev.biochem.052308.105824	journal	June 2010
The structural role of receptor tyrosine sulfation in chemokine recognition: Tyrosine sulfation of chemokine receptors Ludeman, Justin P.; Stone, Martin J. British Journal of Pharmacology, Vol. 171, Issue 5 https://doi.org/10.1111/bph.12455	journal	February 2014
Minimal model systems for β-sheet secondary structure in proteins Gellman, Samuel H. Current Opinion in Chemical Biology, Vol. 2, Issue 6 https://doi.org/10.1016/S1367-5931(98)80109-9	journal	January 1998
Tyrosine sulfation: an increasingly recognised post-translational modification of secreted proteins Stone, Martin J.; Chuang, Sara; Hou, Xu New Biotechnology, Vol. 25, Issue 5 https://doi.org/10.1016/j.nbt.2009.03.011	journal	June 2009
AAV-expressed eCD4-Ig provides durable protection from multiple SHIV challenges Gardner, Matthew R.; Kattenhorn, Lisa M.; Kondur, Hema R. Nature, Vol. 519, Issue 7541 https://doi.org/10.1038/nature14264	journal	February 2015
Tyrosine sulfation in a Gram-negative bacterium Han, Sang-Wook; Lee, Sang-Won; Bahar, Ofir Nature Communications, Vol. 3, Issue 1 https://doi.org/10.1038/ncomms2157	journal	January 2012
Crystal Structure of a Biosynthetic Sulfo-hirudin Complexed to Thrombin Liu, Chang C.; Brustad, Eric; Liu, Wenshe Journal of the American Chemical Society, Vol. 129, Issue 35 https://doi.org/10.1021/ja0735002	journal	September 2007
The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium Pruitt, Rory N.; Schwessinger, Benjamin; Joe, Anna Science Advances, Vol. 1, Issue 6 https://doi.org/10.1126/sciadv.1500245	journal	July 2015
A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli Chatterjee, Abhishek; Sun, Sophie B.; Furman, Jennifer L. Biochemistry, Vol. 52, Issue 10 https://doi.org/10.1021/bi4000244	journal	February 2013
Structures of the CCR5 N Terminus and of a Tyrosine-Sulfated Antibody with HIV-1 gp120 and CD4 Huang, C. -c.; Lam, S. N.; Acharya, P. Science, Vol. 317, Issue 5846 https://doi.org/10.1126/science.1145373	journal	September 2007
Genomically Recoded Organisms Expand Biological Functions Lajoie, M. J.; Rovner, A. J.; Goodman, D. B. Science, Vol. 342, Issue 6156, p. 357-360 https://doi.org/10.1126/science.1241459	journal	October 2013
An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli Young, Travis S.; Ahmad, Insha; Yin, Jun A. Journal of Molecular Biology, Vol. 395, Issue 2 https://doi.org/10.1016/j.jmb.2009.10.030	journal	January 2010
Protein evolution with an expanded genetic code Liu, C. C.; Mack, A. V.; Tsao, M. -L. Proceedings of the National Academy of Sciences, Vol. 105, Issue 46 https://doi.org/10.1073/pnas.0809543105	journal	November 2008
Protein sulfation analysis—A primer Monigatti, Flavio; Hekking, Brian; Steen, Hanno Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1764, Issue 12 https://doi.org/10.1016/j.bbapap.2006.07.002	journal	December 2006
Using circular dichroism spectra to estimate protein secondary structure Greenfield, Norma J Nature Protocols, Vol. 1, Issue 6, p. 2876-2890 https://doi.org/10.1038/nprot.2006.202	journal	January 2007

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