SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study

Journal Article · Tue Sep 19 00:00:00 EDT 2017 · Journal of Biomedical Materials Research. Part A

DOI:https://doi.org/10.1002/jbm.a.36200· OSTI ID:1392697

Yuan, Muzhaozi ^[1]; Wang, Ya  ^[2]; Qin, Yi‐Xian ^[3]

---

1. Heavy Engineering 133, Department of Mechanical Engineering State University of New York at Stony Brook Stony Brook New York 11794‐2300
2. LE 153, Department of Mechanical Engineering State University of New York at Stony Brook Stony Brook New York 11794‐2300
3. Department of Biomedical Engineering, 215 Bioengineering Building State University of New York at Stony Brook Stony Brook New York 11794‐5281

Abstract This work aims to explore the concentration‐dependence of SPIO‐Au core–shell nanoscale particles (NPs) (17.3 ± 1.2 nm in diameter) on biocompatibility and osteogenic differentiation of preosteoblast MC3T3‐E1 cells. The stability of NPs was first investigated by UV–vis absorption spectra and zeta potential measurement. Then concentration effects of NPs (1–80 μg/mL) were evaluated on viability, morphology, proliferation, cellular uptake, and alkaline phosphate (ALP) activity levels. Results have shown strong stability and no acute toxicity (viability > 93%) or morphological difference at all concentration levels of NPs. The proliferation results indicated that the concentration of NPs below 40 μg/mL does not affect the cell proliferation for 7 days of incubation. Transmission electron microscopy images revealed the successful internalization of NPs into MC3T3‐E1 cells and the dose‐dependent accumulation of NPs inside the cytoplasm. The ALP level of MC3T3‐E1 cells was improved by 49% (of control) after treated with NPs at 10 μg/mL for 10 days, indicating their positive effect on early osteogenic differentiation. This study confirmed the excellent biocompatibility of SPIO‐Au NPs and their great potential for promoting osteogenic differentiation and promised the future application for these NPs in bone engineering including drug delivery, cell labeling, and activity tracking within scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3350–3359, 2017.

• Sponsoring Organization: USDOE
• OSTI ID: 1392697
• Journal Information: Journal of Biomedical Materials Research. Part A, Journal Name: Journal of Biomedical Materials Research. Part A Vol. 105 Journal Issue: 12; ISSN 1549-3296
• Publisher: Wiley Blackwell (John Wiley & Sons)
• Country of Publication: United States
• Language: English