The structure of PccH from Geobacter sulfurreducens  - a novel low reduction potential monoheme cytochrome essential for accepting electrons from an electrode

Dantas, Joana M.; Campelo, Luísa M.; Duke, Norma E.  C.; Salgueiro, Carlos A.; Pokkuluri, P. Raj

doi:10.1111/febs.13269

Title: The structure of PccH from Geobacter sulfurreducens - a novel low reduction potential monoheme cytochrome essential for accepting electrons from an electrode

Journal Article · Fri Apr 10 00:00:00 EDT 2015 · Federation of European Biochemical Societies (FEBS) Journal

DOI:https://doi.org/10.1111/febs.13269· OSTI ID:1392071

Dantas, Joana M. ^[1]; Campelo, Luísa M. ^[1]; Duke, Norma E. C. ^[2]; Salgueiro, Carlos A. ^[1]; Pokkuluri, P. Raj ^[2]

UCIBIO - REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica Portugal
Biosciences Division, Argonne National Laboratory, Lemont IL USA

The structure of cytochrome-c (GSU3274) designated as PccH from Geobacter sulfurreducens was determined at 2.0 Å resolution. PccH is a small (15 kDa) cytochrome containing one c-type heme, found to be essential for growth of G. sulfurreducens accepting electrons from graphite electrodes poised at -300 mV versus SHE with fumarate as the terminal electron acceptor. The structure of PccH is unique among the monoheme cytochromes described to date. The structural fold of PccH can be described as forming two lobes with the heme sandwiched in a cleft between the two lobes. In addition, PccH has a low reduction potential of -24 mV at pH 7, which is unusual for monoheme cytochromes. Based on difference in structure together with sequence phylogenetic analysis we propose that PccH can be regarded as a first characterized example of a new subclass of class I monoheme cytochromes. The low reduction potential of PccH may enable the protein to be redox active at the typically negative potential ranges encountered by this bacterium. Because PccH is predicted to be located in the periplasm of G. sulfurreducens, it could not be involved in the first step of accepting electrons from the electrode but very likely involved in the downstream electron transport events in the periplasm.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science - Office of Basic Energy Sciences - Chemical Sciences, Geosciences, and Biosciences Division; USDOE Office of Science - Office of Biological and Environmental Research

DOE Contract Number:: AC02-06CH11357

OSTI ID:: 1392071

Journal Information:: Federation of European Biochemical Societies (FEBS) Journal, Vol. 282, Issue 11; ISSN 1742-464X

Publisher:: Federation of European Biochemical Societies

Country of Publication:: United States

Language:: English

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Title: The structure of PccH from Geobacter sulfurreducens - a novel low reduction potential monoheme cytochrome essential for accepting electrons from an electrode

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