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Title: Liquid Crystal Enabled Early Stage Detection of Beta Amyloid Formation on Lipid Monolayers

Abstract

Liquid crystals (LCs) can serve as sensitive reporters of interfacial events, and this property has been used for sensing of synthetic or biological toxins. Here it is demonstrated that LCs can distinguish distinct molecular motifs and exhibit a specific response to beta-sheet structures. That property is used to detect the formation of highly toxic protofibrils involved in neurodegenerative diseases, where it is crucial to develop methods that probe the early-stage aggregation of amyloidogenic peptides in the vicinity of biological membranes. In the proposed method, the amyloid fibrils formed at the lipid-decorated LC interface can change the orientation of LCs and form elongated and branched structures that are amplified by the mesogenic medium; however, nonamyloidogenic peptides form ellipsoidal domains of tilted LCs. Moreover, a theoretical and computational analysis is used to reveal the underlying structure of the LC, thereby providing a detailed molecular-level view of the interactions and mechanisms responsible for such motifs. The corresponding signatures can be detected at nanomolar concentrations of peptide by polarized light microscopy and much earlier than the ones that can be identified by fluorescence-based techniques. As such, it offers the potential for early diagnoses of neurodegenerative diseases and for facile testing of inhibitors of amyloidmore » formation.« less

Authors:
 [1];  [2];  [1];  [1];  [3];  [2];  [4]
  1. Institute for Molecular Engineering, University of Chicago, Chicago IL 60637 USA
  2. Chemical and Biological Engineering, University of Wisconsin, Madison WI 53706 USA
  3. Institute for Molecular Engineering, University of Chicago, Chicago IL 60637 USA; Departamento de Materiales y Minerales, Facultad de Minas, Universidad Nacional de Colombia, Sede Medellín, Calle 75 # 79A-51, Bloque M17 Medellín Colombia
  4. Institute for Molecular Engineering, University of Chicago, Chicago IL 60637 USA; Argonne National Laboratory, Argonne IL 60439 USA
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science - Office of Basic Energy Sciences - Materials Sciences and Engineering Division; National Science Foundation (NSF); Swiss National Science Foundation (SNSF); University of Wisconsin - Madison - Materials Research Science and Engineering Center (MRSEC)
OSTI Identifier:
1391736
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Advanced Functional Materials
Additional Journal Information:
Journal Volume: 25; Journal Issue: 38; Journal ID: ISSN 1616-301X
Publisher:
Wiley
Country of Publication:
United States
Language:
English

Citation Formats

Sadati, Monirosadat, Apik, Aslin Izmitli, Armas-Perez, Julio C., Martinez-Gonzalez, Jose, Hernandez-Ortiz, Juan P., Abbott, Nicholas L., and de Pablo, Juan J. Liquid Crystal Enabled Early Stage Detection of Beta Amyloid Formation on Lipid Monolayers. United States: N. p., 2015. Web. doi:10.1002/adfm.201502830.
Sadati, Monirosadat, Apik, Aslin Izmitli, Armas-Perez, Julio C., Martinez-Gonzalez, Jose, Hernandez-Ortiz, Juan P., Abbott, Nicholas L., & de Pablo, Juan J. Liquid Crystal Enabled Early Stage Detection of Beta Amyloid Formation on Lipid Monolayers. United States. doi:10.1002/adfm.201502830.
Sadati, Monirosadat, Apik, Aslin Izmitli, Armas-Perez, Julio C., Martinez-Gonzalez, Jose, Hernandez-Ortiz, Juan P., Abbott, Nicholas L., and de Pablo, Juan J. Wed . "Liquid Crystal Enabled Early Stage Detection of Beta Amyloid Formation on Lipid Monolayers". United States. doi:10.1002/adfm.201502830.
@article{osti_1391736,
title = {Liquid Crystal Enabled Early Stage Detection of Beta Amyloid Formation on Lipid Monolayers},
author = {Sadati, Monirosadat and Apik, Aslin Izmitli and Armas-Perez, Julio C. and Martinez-Gonzalez, Jose and Hernandez-Ortiz, Juan P. and Abbott, Nicholas L. and de Pablo, Juan J.},
abstractNote = {Liquid crystals (LCs) can serve as sensitive reporters of interfacial events, and this property has been used for sensing of synthetic or biological toxins. Here it is demonstrated that LCs can distinguish distinct molecular motifs and exhibit a specific response to beta-sheet structures. That property is used to detect the formation of highly toxic protofibrils involved in neurodegenerative diseases, where it is crucial to develop methods that probe the early-stage aggregation of amyloidogenic peptides in the vicinity of biological membranes. In the proposed method, the amyloid fibrils formed at the lipid-decorated LC interface can change the orientation of LCs and form elongated and branched structures that are amplified by the mesogenic medium; however, nonamyloidogenic peptides form ellipsoidal domains of tilted LCs. Moreover, a theoretical and computational analysis is used to reveal the underlying structure of the LC, thereby providing a detailed molecular-level view of the interactions and mechanisms responsible for such motifs. The corresponding signatures can be detected at nanomolar concentrations of peptide by polarized light microscopy and much earlier than the ones that can be identified by fluorescence-based techniques. As such, it offers the potential for early diagnoses of neurodegenerative diseases and for facile testing of inhibitors of amyloid formation.},
doi = {10.1002/adfm.201502830},
journal = {Advanced Functional Materials},
issn = {1616-301X},
number = 38,
volume = 25,
place = {United States},
year = {2015},
month = {9}
}

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