Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins

Wohlever, Matthew L.; Mateja, Agnieszka; McGilvray, Philip T.; Day, Kasey J.; Keenan, Robert J.

doi:10.1016/j.molcel.2017.06.019

Title: Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins

Journal Article · Sat Jul 01 00:00:00 EDT 2017 · Molecular Cell

DOI:https://doi.org/10.1016/j.molcel.2017.06.019· OSTI ID:1461207

Wohlever, Matthew L.; Mateja, Agnieszka; McGilvray, Philip T.; Day, Kasey J.; Keenan, Robert J.

We discuss that mislocalized tail-anchored (TA) proteins of the outer mitochondrial membrane are cleared by a newly identified quality control pathway involving the conserved eukaryotic protein Msp1 (ATAD1 in humans). Msp1 is a transmembrane AAA-ATPase, but its role in TA protein clearance is not known. Here, using purified components reconstituted into proteoliposomes, we show that Msp1 is both necessary and sufficient to drive the ATP-dependent extraction of TA proteins from the membrane. A crystal structure of the Msp1 cytosolic region modeled into a ring hexamer suggests that active Msp1 contains a conserved membrane-facing surface adjacent to a central pore. Structure-guided mutagenesis of the pore residues shows that they are critical for TA protein extraction in vitro and for functional complementation of an msp1 deletion in yeast. Together, these data provide a molecular framework for Msp1-dependent extraction of mislocalized TA proteins from the outer mitochondrial membrane.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Contributing Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Grant/Contract Number:: AC02-06CH11357; NCI CCSG P30 CA060553; S10 RR029205; P41 GM103403; F32GM119194-01; T32 GM007183; 5T32CA9594-25; CCSG P30 CA060553

OSTI ID:: 1461207

Alternate ID(s):: OSTI ID: 1390871

Journal Information:: Molecular Cell, Journal Name: Molecular Cell Vol. 67 Journal Issue: 2; ISSN 1097-2765

Publisher:: Cell Press - ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 70 works

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59 BASIC BIOLOGICAL SCIENCES
AAA ATPase
Msp1
mitochondria
tail-anchored membrane protein
quality control
extraction
mislocalized
ATAD1
reconstitution

Title: Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins

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References (54)

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