skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multiple efficacy studies of an adenovirus-vectored foot-and-mouth disease virus serotype A24 subunit vaccine in cattle using homologous challenge

Journal Article · · Vaccine
; ; ; ; ; ; ; ; ; ; ; ;

Here, the safety and efficacy of an experimental, replication-deficient, human adenovirus-vectored foot-and-mouth disease virus (FMDV) serotype A24 Cruzeiro capsid-based subunit vaccine (AdtA24) was examined in eight independent cattle studies. AdtA24 non-adjuvanted vaccine was administered intramuscularly to a total of 150 steers in doses ranging from approximately 1.0 × 108 to 2.1 × 1011 particle units per animal. No detectable local or systemic reactions were observed after vaccination. At 7 days post-vaccination (dpv), vaccinated and control animals were challenged with FMDV serotype A24 Cruzeiro via the intradermal lingual route. Vaccine efficacy was measured by FMDV A24 serum neutralizing titers and by protection from clinical disease and viremia after challenge. The results of eight studies demonstrated a strong correlation between AdtA24 vaccine dose and protection from clinical disease (R2 = 0.97) and viremia (R2 = 0.98). There was also a strong correlation between FMDV A24 neutralization titers on day of challenge and protection from clinical disease (R2 = 0.99). Vaccination with AdtA24 enabled differentiation of infected from vaccinated animals (DIVA) as demonstrated by the absence of antibodies to the FMDV nonstructural proteins in vaccinates prior to challenge. Lack of AdtA24 vaccine shedding after vaccination was indicated by the absence of neutralizing antibody titers to both the adenovector and FMDV A24 Cruzeiro in control animals after co-mingling with vaccinated cattle for three to four weeks. In summary, a non-adjuvanted AdtA24 experimental vaccine was shown to be safe, immunogenic, consistently protected cattle at 7 dpv against direct, homologous FMDV challenge, and enabled differentiation of infected from vaccinated cattle prior to challenge.

Research Organization:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-06OR23100; HSHQDC-07-9-00004; GS-23F-8006H; HSHQDC-8-C-00011; HSHQPD-07-X-00003
OSTI ID:
1390387
Alternate ID(s):
OSTI ID: 1494695
Journal Information:
Vaccine, Journal Name: Vaccine Vol. 34 Journal Issue: 27; ISSN 0264-410X
Publisher:
ElsevierCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 26 works
Citation information provided by
Web of Science

Cited By (6)

The antibody response induced FMDV vaccines in sheep correlates with early transcriptomic responses in blood journal January 2020
Safety profile of a replication-deficient human adenovirus-vectored foot-and-mouth disease virus serotype A24 subunit vaccine in cattle journal October 2017
The Foot-and-Mouth Disease Carrier State Divergence in Cattle journal May 2016
Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development journal September 2017
Contact Challenge of Cattle with Foot-and-Mouth Disease Virus Validates the Role of the Nasopharyngeal Epithelium as the Site of Primary and Persistent Infection journal December 2018
Efficacy of an adenovirus-vectored foot-and-mouth disease virus serotype A subunit vaccine in cattle using a direct contact transmission model journal August 2018

Figures / Tables (6)

Table 1(p. 3)table Table 1
Figure 1(p. 4)figure Figure 1
Figure 2(p. 5)figure Figure 2
Figure 3(p. 5)figure Figure 3
Figure 4(p. 5)figure Figure 4
Figure 5(p. 6)figure Figure 5

Similar Records

Use of ENABL® adjuvant to increase the potency of an adenovirus-vectored foot-and-mouth disease virus serotype A subunit vaccine
Journal Article · Thu Feb 01 00:00:00 EST 2018 · Vaccine · OSTI ID:1390387
+9 more
Versatility of the adenovirus-vectored foot-and-mouth disease vaccine platform across multiple foot-and-mouth disease virus serotypes and topotypes using a vaccine dose representative of the AdtA24 conditionally licensed vaccine
Journal Article · Sat Oct 13 00:00:00 EDT 2018 · Vaccine · OSTI ID:1390387
+11 more
Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine
Journal Article · Thu Sep 15 00:00:00 EDT 2016 · BMC Veterinary Research · OSTI ID:1390387
+7 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Foot-and-mouth disease virus
FMDV serotype A24 Cruzeiro
Replication-deficient human adenovirus vectored vaccine
DIVA
Vaccine efficacy