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Title: Light harvesting in phototrophic bacteria: structure and function

Authors:
;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1388914
DOE Contract Number:
SC0001035
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical Journal; Journal Volume: 474; Journal Issue: 13; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK
Country of Publication:
United States
Language:
English
Subject:
solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

Citation Formats

Saer, Rafael G., and Blankenship, Robert E.. Light harvesting in phototrophic bacteria: structure and function. United States: N. p., 2017. Web. doi:10.1042/BCJ20160753.
Saer, Rafael G., & Blankenship, Robert E.. Light harvesting in phototrophic bacteria: structure and function. United States. doi:10.1042/BCJ20160753.
Saer, Rafael G., and Blankenship, Robert E.. Tue . "Light harvesting in phototrophic bacteria: structure and function". United States. doi:10.1042/BCJ20160753.
@article{osti_1388914,
title = {Light harvesting in phototrophic bacteria: structure and function},
author = {Saer, Rafael G. and Blankenship, Robert E.},
abstractNote = {},
doi = {10.1042/BCJ20160753},
journal = {Biochemical Journal},
number = 13,
volume = 474,
place = {United States},
year = {Tue Jun 13 00:00:00 EDT 2017},
month = {Tue Jun 13 00:00:00 EDT 2017}
}
  • Photosynthetic organisms have evolved diverse light-harvesting complexes to harness light of various qualities and intensities. Photosynthetic bacteria can have (bacterio)chlorophyll Q y antenna absorption bands ranging from ~650 to ~1100 nm. This broad range of wavelengths has allowed many organisms to thrive in unique light environments. Roseiflexus castenholzii is a niche-adapted, filamentous anoxygenic phototroph (FAP) that lacks chlorosomes, the dominant antenna found in most green bacteria, and here we describe the purification of a full complement of photosynthetic complexes: the light-harvesting (LH) antenna, reaction center (RC), and core complex (RC-LH). By high-performance liquid chromatography separation of bacteriochlorophyll and bacteriopheophytin pigmentsmore » extracted from the core complex and the RC, the number of subunits that comprise the antenna was determined to be 15 ± 1. Resonance Raman spectroscopy of the carbonyl stretching region displayed modes indicating that 3C-acetyl groups of BChl a are all involved in molecular interactions probably similar to those found in LH1 complexes from purple photosynthetic bacteria. Finally, two-dimensional projections of negatively stained core complexes and the LH antenna revealed a closed, slightly elliptical LH ring with an average diameter of 130 ± 10 Å surrounding a single RC that lacks an H-subunit but is associated with a tetraheme c-type cytochrome.« less
  • Great progress in the study of structure and dynamics of photosynthetic light-harvesting pigment-protein complexes has recently resulted in detailed understanding of the light-harvesting and light-conversion processes of photosynthesis. The authors review and discuss recent results on the elementary excitation transfer dynamics of the purple bacterial LH2 peripheral complex. When combining the information from the two LH2 structures that are now available with the experimental results obtained from steady-state spectroscopy, a variety of ultrafast techniques and computer simulations, a detailed understanding of the LH2 function is obtained. Dynamics relevant to the complete photosynthetic unit (PSU = LH2 + LH1 core +more » reaction center), as well as models of the PSU obtained on the basis of the LH2 structure, suggest how the characteristic structural features of LH2 and LH1 have been designed to optimize the overall light-harvesting and trapping process in the PSU.« less