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Title: Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline

Abstract

Nonlinear optical (NLO) instrumentation has been integrated with synchrotron X-ray diffraction (XRD) for combined single-platform analysis, initially targeting applications for automated crystal centering. Second-harmonic-generation microscopy and two-photon-excited ultraviolet fluorescence microscopy were evaluated for crystal detection and assessed by X-ray raster scanning. Two optical designs were constructed and characterized; one positioned downstream of the sample and one integrated into the upstream optical path of the diffractometer. Both instruments enabled protein crystal identification with integration times between 80 and 150 µs per pixel, representing a ~10 3–10 4-fold reduction in the per-pixel exposure time relative to X-ray raster scanning. Quantitative centering and analysis of phenylalanine hydroxylase fromChromobacterium violaceumcPAH,Trichinella spiralisdeubiquitinating enzyme TsUCH37, human κ-opioid receptor complex kOR-T4L produced in lipidic cubic phase (LCP), intimin prepared in LCP, and α-cellulose samples were performed by collecting multiple NLO images. The crystalline samples were characterized by single-crystal diffraction patterns, while α-cellulose was characterized by fiber diffraction. Good agreement was observed between the sample positions identified by NLO and XRD raster measurements for all samples studied.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1385764
DOE Contract Number:  
SC000997
Resource Type:
Journal Article
Journal Name:
Journal of Synchrotron Radiation
Additional Journal Information:
Journal Volume: 20; Journal Issue: 4; Related Information: C3Bio partners with Purdue University (lead); Argonne National Laboratory; National Renewable Energy Laboratory; Northeastern University; University of Tennessee; Journal ID: ISSN 0909-0495
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
catalysis (homogeneous); catalysis (heterogeneous); biofuels (including algae and biomass); bio-inspired; materials and chemistry by design; synthesis (self-assembly); synthesis (scalable processing); XRD; NLO; SHG; SONICC; centering; protein; TPE-UVF; microscopy; LCP; two-photon

Citation Formats

Madden, Jeremy T., Toth, Scott J., Dettmar, Christopher M., Newman, Justin A., Oglesbee, Robert A., Hedderich, Hartmut G., Everly, R. Michael, Becker, Michael, Ronau, Judith A., Buchanan, Susan K., Cherezov, Vadim, Morrow, Marie E., Xu, Shenglan, Ferguson, Dale, Makarov, Oleg, Das, Chittaranjan, Fischetti, Robert, and Simpson, Garth J. Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline. United States: N. p., 2013. Web. doi:10.1107/S0909049513007942.
Madden, Jeremy T., Toth, Scott J., Dettmar, Christopher M., Newman, Justin A., Oglesbee, Robert A., Hedderich, Hartmut G., Everly, R. Michael, Becker, Michael, Ronau, Judith A., Buchanan, Susan K., Cherezov, Vadim, Morrow, Marie E., Xu, Shenglan, Ferguson, Dale, Makarov, Oleg, Das, Chittaranjan, Fischetti, Robert, & Simpson, Garth J. Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline. United States. doi:10.1107/S0909049513007942.
Madden, Jeremy T., Toth, Scott J., Dettmar, Christopher M., Newman, Justin A., Oglesbee, Robert A., Hedderich, Hartmut G., Everly, R. Michael, Becker, Michael, Ronau, Judith A., Buchanan, Susan K., Cherezov, Vadim, Morrow, Marie E., Xu, Shenglan, Ferguson, Dale, Makarov, Oleg, Das, Chittaranjan, Fischetti, Robert, and Simpson, Garth J. Fri . "Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline". United States. doi:10.1107/S0909049513007942.
@article{osti_1385764,
title = {Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline},
author = {Madden, Jeremy T. and Toth, Scott J. and Dettmar, Christopher M. and Newman, Justin A. and Oglesbee, Robert A. and Hedderich, Hartmut G. and Everly, R. Michael and Becker, Michael and Ronau, Judith A. and Buchanan, Susan K. and Cherezov, Vadim and Morrow, Marie E. and Xu, Shenglan and Ferguson, Dale and Makarov, Oleg and Das, Chittaranjan and Fischetti, Robert and Simpson, Garth J.},
abstractNote = {Nonlinear optical (NLO) instrumentation has been integrated with synchrotron X-ray diffraction (XRD) for combined single-platform analysis, initially targeting applications for automated crystal centering. Second-harmonic-generation microscopy and two-photon-excited ultraviolet fluorescence microscopy were evaluated for crystal detection and assessed by X-ray raster scanning. Two optical designs were constructed and characterized; one positioned downstream of the sample and one integrated into the upstream optical path of the diffractometer. Both instruments enabled protein crystal identification with integration times between 80 and 150 µs per pixel, representing a ~103–104-fold reduction in the per-pixel exposure time relative to X-ray raster scanning. Quantitative centering and analysis of phenylalanine hydroxylase fromChromobacterium violaceumcPAH,Trichinella spiralisdeubiquitinating enzyme TsUCH37, human κ-opioid receptor complex kOR-T4L produced in lipidic cubic phase (LCP), intimin prepared in LCP, and α-cellulose samples were performed by collecting multiple NLO images. The crystalline samples were characterized by single-crystal diffraction patterns, while α-cellulose was characterized by fiber diffraction. Good agreement was observed between the sample positions identified by NLO and XRD raster measurements for all samples studied.},
doi = {10.1107/S0909049513007942},
journal = {Journal of Synchrotron Radiation},
issn = {0909-0495},
number = 4,
volume = 20,
place = {United States},
year = {2013},
month = {5}
}

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