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Title: Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1384847
DOE Contract Number:  
SC0001035
Resource Type:
Journal Article
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 289; Journal Issue: 43; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

Citation Formats

Olsen, John D., Adams, Peter G., Jackson, Philip J., Dickman, Mark J., Qian, Pu, and Hunter, C. Neil. Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy. United States: N. p., 2014. Web. doi:10.1074/jbc.M114.596585.
Olsen, John D., Adams, Peter G., Jackson, Philip J., Dickman, Mark J., Qian, Pu, & Hunter, C. Neil. Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy. United States. doi:10.1074/jbc.M114.596585.
Olsen, John D., Adams, Peter G., Jackson, Philip J., Dickman, Mark J., Qian, Pu, and Hunter, C. Neil. Fri . "Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy". United States. doi:10.1074/jbc.M114.596585.
@article{osti_1384847,
title = {Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy},
author = {Olsen, John D. and Adams, Peter G. and Jackson, Philip J. and Dickman, Mark J. and Qian, Pu and Hunter, C. Neil},
abstractNote = {},
doi = {10.1074/jbc.M114.596585},
journal = {Journal of Biological Chemistry},
issn = {0021-9258},
number = 43,
volume = 289,
place = {United States},
year = {2014},
month = {9}
}

Works referenced in this record:

Experimental evidence that the membrane-spanning helix of PufX adopts a bent conformation that facilitates dimerisation of the Rhodobacter sphaeroides RC–LH1 complex through N-terminal interactions
journal, January 2011

  • Ratcliffe, Emma C.; Tunnicliffe, Richard B.; Ng, Irene W.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 1
  • DOI: 10.1016/j.bbabio.2010.10.003

Isolation, Size Estimates, and Spectral Heterogeneity of an Oligomeric Series of Light-Harvesting 1 Complexes from Rhodobacter sphaeroides
journal, July 2002

  • Westerhuis, Willem H. J.; Sturgis, James N.; Ratcliffe, Emma C.
  • Biochemistry, Vol. 41, Issue 27
  • DOI: 10.1021/bi011663b

Probing protein structural requirements for formation of the core light-harvesting complex of photosynthetic bacteria using hybrid reconstitution methodology
journal, June 1994

  • Loach, Paul A.; Parkes-Loach, Pamela S.; Davis, Christine M.
  • Photosynthesis Research, Vol. 40, Issue 3
  • DOI: 10.1007/BF00034773

Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides : LH2 at 6 Å, LH1 and RC-LH1 at 25 Å 1 1Edited by K. Nagai
journal, October 1998

  • Walz, Thomas; Jamieson, Stuart J.; Bowers, Claire M.
  • Journal of Molecular Biology, Vol. 282, Issue 4
  • DOI: 10.1006/jmbi.1998.2050

The Rhodobacter sphaeroides PufX protein is not required for photosynthetic competence in the absence of a light harvesting system
journal, August 1994


The long-range organization of a native photosynthetic membrane
journal, December 2004

  • Frese, R. N.; Siebert, C. A.; Niederman, R. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 52
  • DOI: 10.1073/pnas.0407295102

Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM
journal, February 2003

  • Scheuring, S.; Seguin, J.; Marco, S.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 4
  • DOI: 10.1073/pnas.0437992100

The LH1–RC core complex of Rhodobacter sphaeroides: interaction between components, time-dependent assembly, and topology of the PufX protein
journal, September 1998

  • Pugh, Rachel J.; McGlynn, Peter; Jones, Michael R.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1366, Issue 3
  • DOI: 10.1016/S0005-2728(98)00131-5

Watching the components of photosynthetic bacterial membranes and their in situ organisation by atomic force microscopy
journal, July 2005

  • Scheuring, Simon; Lévy, Daniel; Rigaud, Jean-Louis
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1712, Issue 2
  • DOI: 10.1016/j.bbamem.2005.04.005

The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium Synechocystis sp. PCC 6803
journal, November 2011

  • Komenda, Josef; Knoppová, Jana; Kopečná, Jana
  • Plant Physiology, Vol. 158, Issue 1
  • DOI: 10.1104/pp.111.184184

Atomic Force Microscopy Studies of Native Photosynthetic Membranes
journal, May 2009

  • Sturgis, James N.; Tucker, Jaimey D.; Olsen, John D.
  • Biochemistry, Vol. 48, Issue 17
  • DOI: 10.1021/bi900045x

Quantitative proteomic analysis of intracytoplasmic membrane development in Rhodobacter sphaeroides: Proteomics of membrane assembly in Rba. sphaeroides
journal, May 2012


Monomeric RC–LH1 core complexes retard LH2 assembly and intracytoplasmic membrane formation in PufX-minus mutants of Rhodobacter sphaeroides
journal, September 2011

  • Adams, Peter G.; Mothersole, David J.; Ng, Irene W.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 9
  • DOI: 10.1016/j.bbabio.2011.05.019

The 8.5Å Projection Structure of the Core RC–LH1–PufX Dimer of Rhodobacter sphaeroides
journal, June 2005


The native architecture of a photosynthetic membrane
journal, August 2004

  • Bahatyrova, Svetlana; Frese, Raoul N.; Siebert, C. Alistair
  • Nature, Vol. 430, Issue 7003
  • DOI: 10.1038/nature02823

The Photosynthetic Apparatus of Rhodopseudomonas palustris: Structures and Organization
journal, April 2006

  • Scheuring, Simon; Gonçalves, Rui Pedro; Prima, Valérie
  • Journal of Molecular Biology, Vol. 358, Issue 1
  • DOI: 10.1016/j.jmb.2006.01.085

Excitation transfer connectivity in different purple bacteria: A theoretical and experimental study
journal, November 2010

  • de Rivoyre, Matthieu; Ginet, Nicolas; Bouyer, Pierre
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1797, Issue 11
  • DOI: 10.1016/j.bbabio.2010.07.011

Accumulation of the D2 Protein Is a Key Regulatory Step for Assembly of the Photosystem II Reaction Center Complex in Synechocystis PCC 6803
journal, September 2004

  • Komenda, Josef; Reisinger, Veronika; Müller, Bernd Christian
  • Journal of Biological Chemistry, Vol. 279, Issue 47
  • DOI: 10.1074/jbc.M405725200

Three-Dimensional Structure of the Rhodobacter sphaeroides RC-LH1-PufX Complex: Dimerization and Quinone Channels Promoted by PufX
journal, October 2013

  • Qian, Pu; Papiz, Miroslav Z.; Jackson, Philip J.
  • Biochemistry, Vol. 52, Issue 43
  • DOI: 10.1021/bi4011946

The Organization of LH2 Complexes in Membranes from Rhodobacter sphaeroides
journal, August 2008

  • Olsen, John D.; Tucker, Jaimey D.; Timney, John A.
  • Journal of Biological Chemistry, Vol. 283, Issue 45
  • DOI: 10.1074/jbc.M804824200

Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803
journal, November 2008

  • Dobáková, Marika; Sobotka, Roman; Tichý, Martin
  • Plant Physiology, Vol. 149, Issue 2
  • DOI: 10.1104/pp.108.130039

Carotenoids are essential for normal levels of dimerisation of the RC–LH1–PufX core complex of Rhodobacter sphaeroides: Characterisation of R-26 as a crtB (phytoene synthase) mutant
journal, September 2011

  • Ng, Irene W.; Adams, Peter G.; Mothersole, David J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 9
  • DOI: 10.1016/j.bbabio.2011.05.020

Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX
journal, February 2004


Structural Analysis of the Reaction Center Light-harvesting Complex I Photosynthetic Core Complex of Rhodospirillum rubrum Using Atomic Force Microscopy
journal, October 2003

  • Fotiadis, Dimitrios; Qian, Pu; Philippsen, Ansgar
  • Journal of Biological Chemistry, Vol. 279, Issue 3
  • DOI: 10.1074/jbc.M310382200

Variable LH2 stoichiometry and core clustering in native membranes of Rhodospirillum photometricum
journal, September 2004

  • Scheuring, Simon; Rigaud, Jean-Louis; Sturgis, James N.
  • The EMBO Journal, Vol. 23, Issue 21
  • DOI: 10.1038/sj.emboj.7600429

Flexibility and Size Heterogeneity of the LH1 Light Harvesting Complex Revealed by Atomic Force Microscopy: FUNCTIONAL SIGNIFICANCE FOR BACTERIAL PHOTOSYNTHESIS
journal, March 2004

  • Bahatyrova, Svetlana; Frese, Raoul N.; van der Werf, Kees O.
  • Journal of Biological Chemistry, Vol. 279, Issue 20
  • DOI: 10.1074/jbc.M313039200