skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Chemical imaging analysis of the brain with X-ray methods

Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
Grant/Contract Number:
Resource Type:
Journal Article: Published Article
Journal Name:
Spectrochimica Acta. Part B, Atomic Spectroscopy
Additional Journal Information:
Journal Volume: 130; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-12-09 01:50:11; Journal ID: ISSN 0584-8547
Country of Publication:
United Kingdom

Citation Formats

Collingwood, Joanna F., and Adams, Freddy. Chemical imaging analysis of the brain with X-ray methods. United Kingdom: N. p., 2017. Web. doi:10.1016/j.sab.2017.02.013.
Collingwood, Joanna F., & Adams, Freddy. Chemical imaging analysis of the brain with X-ray methods. United Kingdom. doi:10.1016/j.sab.2017.02.013.
Collingwood, Joanna F., and Adams, Freddy. Sat . "Chemical imaging analysis of the brain with X-ray methods". United Kingdom. doi:10.1016/j.sab.2017.02.013.
title = {Chemical imaging analysis of the brain with X-ray methods},
author = {Collingwood, Joanna F. and Adams, Freddy},
abstractNote = {},
doi = {10.1016/j.sab.2017.02.013},
journal = {Spectrochimica Acta. Part B, Atomic Spectroscopy},
number = C,
volume = 130,
place = {United Kingdom},
year = {Sat Apr 01 00:00:00 EDT 2017},
month = {Sat Apr 01 00:00:00 EDT 2017}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.sab.2017.02.013

Citation Metrics:
Cited by: 3works
Citation information provided by
Web of Science

Save / Share:
  • Most interesting materials in nature are heterogeneous, so it is useful to have analytical techniques with spatial resolution sufficient to resolve these heterogeneities.This article presents the basics of X-ray photon-in/photon-out chemical imaging. This family of methods allows one to derive images reflectingthe chemical state of a given element in a complex sample, at micron or deep sub-micron scale. X-ray chemical imaging is relatively non-destructiveand element-selective, and requires minimal sample preparation. The article presents the basic concepts and some considerations of data takingand data analysis, along with some examples.
  • The oxidation layer formed on the surface of Aluminum brass corroded in sea water at 40/sup 0/C for 15 days was analyzed by using different techniques (chemical analysis after selective dissolution, X-ray diffractometry and Auger spectroscopy) and was found to be formed by Cu oxychlorides, basic Zn sulfates, Al and Mg carbonates, Al oxides. Chemical and diffractometric analyses yield an average value of the composition of the corrosion layer, whose thickness should be greater than 60-80/sup 0/ A for chemical and than 100 A for diffractometric analysis. Auger spectroscopy permits the analysis of thin films and of small surface areas,more » and gives results that agree with those of the other two techniques when the corrosion layer is homogeneous. Owing to the complexity of the oxidation layer, where many compounds of the same element with the same valence can be present, the results of Auger spectroscopy should be confirmed with those obtained with one or both of the other two technique. Furthermore, some compounds (as CO/sub 3//sup - -/) cannot be measured by Auger analysis and require the use of chemical methods.« less
  • X-ray photoelectron spectroscopy (XPS) is widely used for characterization of surfaces of materials. Elements in the sample (with the exception of hydrogen and helium) are identified from comparisons of the binding energies of their core levels, determined from measured photoelectron spectra, with tabulated values of these binding energies for the various elements. Information on the chemical state of the detected elements can frequently be obtained from small variations (typically between 0.1 eV and 10 eV) of the core-level binding energies from the corresponding values for the pure elements. Reliable determination of chemical shifts often requires that the binding-energy scale ofmore » the XPS instrument be calibrated with an uncertainty that could be as small as 0.1 eV. The surface potential of an insulating specimen will generally change during an XPS measurement due to surface charging, and it is then difficult to determine binding energies with the accuracy needed for elemental identification or chemical-state determination. There are two steps in dealing with this problem. First, experimental steps can be taken to minimize the amount of surface charging (charge-control methods). Second, corrections for the effects of surface charging can be made after acquisition of the XPS data (charge-correction methods). Although the buildup of surface charge can complicate analysis in some circumstances, it can be creatively used as a tool to gain information about a specimen.« less
  • The clinical diagnosis of many neurodegenerative disorders relies primarily or exclusively on observed behaviors rather than measurable physical tests. One of the hallmarks of Alzheimer disease (AD) is the presence of amyloid-containing plaques associated with deposits of iron, copper and/or zinc. Work in other laboratories has shown that iron-rich plaques can be seen in the mouse brain in vivo with magnetic resonance imaging (MRI) using a high-field strength magnet but this iron cannot be visualized in humans using clinical magnets. To improve the interpretation of MRI, we correlated iron accumulation visualized by X-ray fluorescence spectroscopy, an element-specific technique with T1,more » T2, and susceptibility weighted MR (SWI) in a mouse model of AD. We show that SWI best shows areas of increased iron accumulation when compared to standard sequences.« less
  • Using a non-radioactive iodine-127 labeled cerebral perfusion agent (I-127 IMP), fluorescent X-ray computed tomography (FXCT) clearly revealed the cross-sectional distribution of I-127 IMP in normal mouse brain in-vivo. Cerebral perfusion of cortex and basal ganglion was depicted with 1 mm in-plane spatial resolution and 0.1 mm slice thickness. Degree of cerebral perfusion in basal ganglion was about 2-fold higher than that in cortical regions. This result suggests that in-vivo cerebral perfusion imaging is realized quantitatively by FXCT at high volumetric resolution.