Engineered polyketides: Synergy between protein and host level engineering

Barajas, Jesus F.; Blake-Hedges, Jacquelyn M.; Bailey, Constance B.; Curran, Samuel; Keasling, Jay. D.

doi:10.1016/j.synbio.2017.08.005

Title: Engineered polyketides: Synergy between protein and host level engineering

Journal Article · Fri Sep 01 00:00:00 EDT 2017 · Synthetic and Systems Biotechnology

DOI:https://doi.org/10.1016/j.synbio.2017.08.005· OSTI ID:1380029

Barajas, Jesus F.; Blake-Hedges, Jacquelyn M.; Bailey, Constance B.; Curran, Samuel; Keasling, Jay. D.

Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides encompass one of the largest classes of chemically diverse natural products. With thousands of known polyketides, modular polyketide synthases (PKSs) share a particularly attractive biosynthetic logic for generating chemical diversity. The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds. In this review, we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures, and we examine future applications of PKSs in the production of medicines, fuels and other industrially relevant chemicals.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Vehicle Technologies Office; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC02-05CH11231; AC02- 05CH11231; DGE 1106400; MCB-1442724; DGE-1106400; CBET-1437775

OSTI ID:: 1380029

Alternate ID(s):: OSTI ID: 1629064

Journal Information:: Synthetic and Systems Biotechnology, Journal Name: Synthetic and Systems Biotechnology Vol. 2 Journal Issue: 3; ISSN 2405-805X

Publisher:: ElsevierCopyright Statement

Country of Publication:: China

Language:: English

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Acyl-coenzyme A:(holo-acyl carrier protein) transacylase enzymes as templates for engineering Marcella, Aaron M.; Barb, Adam W. Applied Microbiology and Biotechnology, Vol. 102, Issue 15 https://doi.org/10.1007/s00253-018-9114-2	journal	June 2018
Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases Barajas, Jesus F.; McAndrew, Ryan P.; Thompson, Mitchell G. Journal of Industrial Microbiology & Biotechnology, Vol. 46, Issue 8 https://doi.org/10.1007/s10295-019-02189-z	journal	May 2019
Technical Advances to Accelerate Modular Type I Polyketide Synthase Engineering towards a Retro-biosynthetic Platform Pang, Bo; Valencia, Luis E.; Wang, Jessica Biotechnology and Bioprocess Engineering, Vol. 24, Issue 3 https://doi.org/10.1007/s12257-019-0083-9	journal	June 2019
Emulating evolutionary processes to morph aureothin-type modular polyketide synthases and associated oxygenases Peng, Huiyun; Ishida, Keishi; Sugimoto, Yuki Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-11896-1	journal	September 2019
Site-Specific Labelling of Multidomain Proteins by Amber Codon Suppression Heil, Christina S.; Rittner, Alexander; Goebel, Bjarne Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-33115-5	journal	October 2018
Diversification of polyketide structures via synthase engineering Kornfuehrer, Taylor; Eustáquio, Alessandra S. MedChemComm, Vol. 10, Issue 8 https://doi.org/10.1039/c9md00141g	journal	January 2019
Evolutionary dynamics of natural product biosynthesis in bacteria Chevrette, Marc G.; Gutiérrez-García, Karina; Selem-Mojica, Nelly Natural Product Reports, Vol. 37, Issue 4 https://doi.org/10.1039/c9np00048h	journal	January 2020
Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in Saccharopolyspora erythraea Wu, Hang; Chu, Zuling; Zhang, Wanxiang Journal of Biological Engineering, Vol. 13, Issue 1 https://doi.org/10.1186/s13036-018-0135-2	journal	January 2019

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Accelerating polyketide synthase engineering for high TRY production of biofuels and bioproducts: CRADA Final Report

Technical Report · Wed Sep 13 00:00:00 EDT 2023 · OSTI ID:1380029

Backman, Tyler

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
42 ENGINEERING
polyketide
polyketide synthase
natural products
commodity chemical
metabolic engineering
synthetic biology

Title: Engineered polyketides: Synergy between protein and host level engineering

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