skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae

Abstract

The rice immune receptor XA21 confers resistance to the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). To elucidate the mechanism of XA21-mediated immunity, we previously performed a yeast two-hybrid screening for XA21 interactors and identified XA21 binding protein 21 (XB21). Here, we report that XB21 is an auxilin-like protein predicted to function in clathrin-mediated endocytosis. We demonstrate an XA21/XB21 in vivo interaction using co-immunoprecipitation in rice. Overexpression of XB21 in rice variety Kitaake and a Kitaake transgenic line expressing XA21 confers a necrotic lesion phenotype and enhances resistance to Xoo. RNA sequencing reveals that XB21 overexpression results in the differential expression of 8735 genes (4939 genes up- and 3846 genes down-regulated) (≥2-folds, FDR ≤0.01). The up-regulated genes include those predicted to be involved in ‘cell death’ and ‘vesicle-mediated transport’. These results indicate that XB21 plays a role in the plant immune response and in regulation of cell death. The up-regulation of genes controlling ‘vesicle-mediated transport’ in XB21 overexpression lines is consistent with a functional role for XB21 as an auxilin.

Authors:
 [1];  [2];  [3];  [2]
  1. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Sejong Univ., Seoul (Korea, Republic of). Dept. of Bioresources Engineering and the Plant Engineering Research Inst.
  2. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst., Feedstocks Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology
  3. Univ. of California, Davis, CA (United States). Dept. of Plant Pathology and the Genome Center; Jawaharlal Nehru Univ., New Delhi (India). School of Computational & Integrative Sciences
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF); National Institutes of Health (NIH)
OSTI Identifier:
1379882
Grant/Contract Number:  
AC02-05CH11231; IOS-0817738; GM55962
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Rice
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1939-8425
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Auxilin; Auxilin-Like Protein; Disease Resistance; Heat Shock Cognate Protein; Heat Shock Protein; XA21 Immune Receptor; XA21 Binding Protein 21; Xanthomonas oryzae pv. oryzae

Citation Formats

Park, Chang-Jin, Wei, Tong, Sharma, Rita, and Ronald, Pamela C. Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae. United States: N. p., 2017. Web. doi:10.1186/s12284-017-0166-1.
Park, Chang-Jin, Wei, Tong, Sharma, Rita, & Ronald, Pamela C. Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae. United States. doi:10.1186/s12284-017-0166-1.
Park, Chang-Jin, Wei, Tong, Sharma, Rita, and Ronald, Pamela C. Fri . "Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae". United States. doi:10.1186/s12284-017-0166-1. https://www.osti.gov/servlets/purl/1379882.
@article{osti_1379882,
title = {Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae},
author = {Park, Chang-Jin and Wei, Tong and Sharma, Rita and Ronald, Pamela C.},
abstractNote = {The rice immune receptor XA21 confers resistance to the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). To elucidate the mechanism of XA21-mediated immunity, we previously performed a yeast two-hybrid screening for XA21 interactors and identified XA21 binding protein 21 (XB21). Here, we report that XB21 is an auxilin-like protein predicted to function in clathrin-mediated endocytosis. We demonstrate an XA21/XB21 in vivo interaction using co-immunoprecipitation in rice. Overexpression of XB21 in rice variety Kitaake and a Kitaake transgenic line expressing XA21 confers a necrotic lesion phenotype and enhances resistance to Xoo. RNA sequencing reveals that XB21 overexpression results in the differential expression of 8735 genes (4939 genes up- and 3846 genes down-regulated) (≥2-folds, FDR ≤0.01). The up-regulated genes include those predicted to be involved in ‘cell death’ and ‘vesicle-mediated transport’. These results indicate that XB21 plays a role in the plant immune response and in regulation of cell death. The up-regulation of genes controlling ‘vesicle-mediated transport’ in XB21 overexpression lines is consistent with a functional role for XB21 as an auxilin.},
doi = {10.1186/s12284-017-0166-1},
journal = {Rice},
number = 1,
volume = 10,
place = {United States},
year = {Fri Jun 02 00:00:00 EDT 2017},
month = {Fri Jun 02 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Towards Establishment of a Rice Stress Response Interactome
journal, April 2011


mapman: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes
journal, March 2004