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Title: Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays

Abstract

In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self-organise to form large, functional structures. A comprehensive set of rules governing mesoscale self-organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X-ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high-resolution structural information with precise molecular localisation data.

Authors:
 [1];  [1];  [1];  [1];  [2];  [2]
  1. Univ. of California, San Francisco, CA (United States)
  2. Univ. of California, San Francisco, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1379651
Alternate Identifier(s):
OSTI ID: 1401243
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biology of the Cell
Additional Journal Information:
Journal Volume: 109; Journal Issue: 1; Journal ID: ISSN 0248-4900
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ekman, Axel A., Chen, Jian-Hua, Guo, Jessica, McDermott, Gerry, Le Gros, Mark A., and Larabell, Carolyn A. Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays. United States: N. p., 2016. Web. doi:10.1111/boc.201600044.
Ekman, Axel A., Chen, Jian-Hua, Guo, Jessica, McDermott, Gerry, Le Gros, Mark A., & Larabell, Carolyn A. Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays. United States. doi:10.1111/boc.201600044.
Ekman, Axel A., Chen, Jian-Hua, Guo, Jessica, McDermott, Gerry, Le Gros, Mark A., and Larabell, Carolyn A. Mon . "Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays". United States. doi:10.1111/boc.201600044. https://www.osti.gov/servlets/purl/1379651.
@article{osti_1379651,
title = {Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays},
author = {Ekman, Axel A. and Chen, Jian-Hua and Guo, Jessica and McDermott, Gerry and Le Gros, Mark A. and Larabell, Carolyn A.},
abstractNote = {In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self-organise to form large, functional structures. A comprehensive set of rules governing mesoscale self-organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X-ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high-resolution structural information with precise molecular localisation data.},
doi = {10.1111/boc.201600044},
journal = {Biology of the Cell},
number = 1,
volume = 109,
place = {United States},
year = {Mon Nov 14 00:00:00 EST 2016},
month = {Mon Nov 14 00:00:00 EST 2016}
}

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Works referenced in this record:

Robust uncertainty principles: exact signal reconstruction from highly incomplete frequency information
journal, February 2006

  • Candes, E.J.; Romberg, J.; Tao, T.
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