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Title: Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate

Abstract

Our aims were to identify thermophilic microbial communities that degrade green waste in the presence of the ionic liquids (IL) tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate and examine preservation methods for IL-tolerant communities. High-solids incubations with stepwise increases in IL concentration were conducted to enrich for thermophilic IL-tolerant communities that decomposed green waste. 16S rRNA sequencing of enriched communities revealed microorganisms capable of tolerating high levels of IL. Furthermore, cryogenic preservation of enriched communities reduced the IL tolerance of the community and decreased the relative abundance of IL-tolerant organisms. The use of cryoprotectants did not have an effect on microbial activity on green waste of the stored community. A successful approach was developed to enrich communities that decompose green waste in thermophilic high-solids environments in the presence of IL. Alternative community storage and revival methods are necessary for maintenance and recovery of IL-tolerant communities. The enriched communities provide a targeted source of enzymes for the bioconversion of IL-pretreated green waste for conversion to biofuels.

Authors:
 [1];  [1];  [1];  [2];  [3];  [4];  [5];  [6]
  1. Univ. of California, Davis, CA (United States). Dept. of Biological and Agricultural Engineering
  2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  3. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biological and Materials Science
  4. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Sciences Division
  5. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
  6. Univ. of California, Davis, CA (United States). Dept. of Biological and Agricultural Engineering; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
OSTI Identifier:
1379372
Alternate Identifier(s):
OSTI ID: 1458690
Report Number(s):
LLNL-JRNL-737584
Journal ID: ISSN 0175-7598; ark:/13030/qt0x49p8n0
Grant/Contract Number:  
AC02-05CH11231; DGE-0948021; AC52-07NA27344
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 100; Journal Issue: 12; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; phosphonium ionic liquids; microbial communities; bioenergy; high-solids; pretreatment; storage

Citation Formats

Pace, Sara, Ceballos, Shannon J., Harrold, Duff, Stannard, Whitney, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., and VanderGheynst, Jean S.. Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate. United States: N. p., 2016. Web. doi:10.1007/s00253-016-7525-5.
Pace, Sara, Ceballos, Shannon J., Harrold, Duff, Stannard, Whitney, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., & VanderGheynst, Jean S.. Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate. United States. doi:10.1007/s00253-016-7525-5.
Pace, Sara, Ceballos, Shannon J., Harrold, Duff, Stannard, Whitney, Simmons, Blake A., Singer, Steven W., Thelen, Michael P., and VanderGheynst, Jean S.. Fri . "Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate". United States. doi:10.1007/s00253-016-7525-5. https://www.osti.gov/servlets/purl/1379372.
@article{osti_1379372,
title = {Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate},
author = {Pace, Sara and Ceballos, Shannon J. and Harrold, Duff and Stannard, Whitney and Simmons, Blake A. and Singer, Steven W. and Thelen, Michael P. and VanderGheynst, Jean S.},
abstractNote = {Our aims were to identify thermophilic microbial communities that degrade green waste in the presence of the ionic liquids (IL) tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate and examine preservation methods for IL-tolerant communities. High-solids incubations with stepwise increases in IL concentration were conducted to enrich for thermophilic IL-tolerant communities that decomposed green waste. 16S rRNA sequencing of enriched communities revealed microorganisms capable of tolerating high levels of IL. Furthermore, cryogenic preservation of enriched communities reduced the IL tolerance of the community and decreased the relative abundance of IL-tolerant organisms. The use of cryoprotectants did not have an effect on microbial activity on green waste of the stored community. A successful approach was developed to enrich communities that decompose green waste in thermophilic high-solids environments in the presence of IL. Alternative community storage and revival methods are necessary for maintenance and recovery of IL-tolerant communities. The enriched communities provide a targeted source of enzymes for the bioconversion of IL-pretreated green waste for conversion to biofuels.},
doi = {10.1007/s00253-016-7525-5},
journal = {Applied Microbiology and Biotechnology},
number = 12,
volume = 100,
place = {United States},
year = {Fri Apr 22 00:00:00 EDT 2016},
month = {Fri Apr 22 00:00:00 EDT 2016}
}

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Cited by: 3 works
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Works referenced in this record:

Feedstocks for Lignocellulosic Biofuels
journal, August 2010

  • Somerville, Cris; Youngs, Heather; Taylor, Caroline
  • Science, Vol. 329, Issue 5993, p. 790-792
  • DOI: 10.1126/science.1189268