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Title: Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance

Abstract

Background: Grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. However, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. Deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. The varied tissue-specific distribution of cell wall components adds complexity to the origins of cell wall recalcitrance in grasses. This complexity usually led to empirically developed pretreatment processes to overcome recalcitrance. A further complication is that efficient pretreatment procedures generally treat the less recalcitrant tissues more than necessary, which results in the generation of undesirable biomass degradation products. Results: Six different sugarcane hybrids were used as model grasses to evaluate the tissue-specific distribution of hemicelluloses and the role of these components in cell wall recalcitrance. Acetylated glucuronoarabinoxylan (GAX) occurs in all tissues. Mixed-linkage glucan (MLG) was relevant in the innermost regions of the sugarcane internodes (up to 15.4 % w/w), especially in the low-lignin content hybrids. Immunofluorescence microscopy showed that xylans predominated in vascular bundles, whereas MLG occurred mostly in the parenchyma cell walls from the pith region of the hybridsmore » with low-lignin content. Evaluation of the digestibility of sugarcane polysaccharides by commercial enzymes indicated that the cell wall recalcitrance varied considerably along the internode regions and in the sugarcane hybrids. Pith regions of the hybrids with high MLG and low-lignin contents reached up to 85 % cellulose conversion after 72 h of hydrolysis, without any pretreatment. Conclusions: The collective characteristics of the internode regions were related to the varied recalcitrance found in the samples. Components such as lignin and GAX were critical for the increased recalcitrance, but low cellulose crystallinity index, high MLG contents, and highly substituted GAX contributed to the generation of a less recalcitrant material.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618639
Alternate Identifier(s):
OSTI ID: 1379329
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Costa, Thales H. F., Vega-Sánchez, Miguel E., Milagres, Adriane M. F., Scheller, Henrik V., and Ferraz, André. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. Netherlands: N. p., 2016. Web. doi:10.1186/s13068-016-0513-2.
Costa, Thales H. F., Vega-Sánchez, Miguel E., Milagres, Adriane M. F., Scheller, Henrik V., & Ferraz, André. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. Netherlands. https://doi.org/10.1186/s13068-016-0513-2
Costa, Thales H. F., Vega-Sánchez, Miguel E., Milagres, Adriane M. F., Scheller, Henrik V., and Ferraz, André. 2016. "Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance". Netherlands. https://doi.org/10.1186/s13068-016-0513-2.
@article{osti_1618639,
title = {Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance},
author = {Costa, Thales H. F. and Vega-Sánchez, Miguel E. and Milagres, Adriane M. F. and Scheller, Henrik V. and Ferraz, André},
abstractNote = {Background: Grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. However, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. Deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. The varied tissue-specific distribution of cell wall components adds complexity to the origins of cell wall recalcitrance in grasses. This complexity usually led to empirically developed pretreatment processes to overcome recalcitrance. A further complication is that efficient pretreatment procedures generally treat the less recalcitrant tissues more than necessary, which results in the generation of undesirable biomass degradation products. Results: Six different sugarcane hybrids were used as model grasses to evaluate the tissue-specific distribution of hemicelluloses and the role of these components in cell wall recalcitrance. Acetylated glucuronoarabinoxylan (GAX) occurs in all tissues. Mixed-linkage glucan (MLG) was relevant in the innermost regions of the sugarcane internodes (up to 15.4 % w/w), especially in the low-lignin content hybrids. Immunofluorescence microscopy showed that xylans predominated in vascular bundles, whereas MLG occurred mostly in the parenchyma cell walls from the pith region of the hybrids with low-lignin content. Evaluation of the digestibility of sugarcane polysaccharides by commercial enzymes indicated that the cell wall recalcitrance varied considerably along the internode regions and in the sugarcane hybrids. Pith regions of the hybrids with high MLG and low-lignin contents reached up to 85 % cellulose conversion after 72 h of hydrolysis, without any pretreatment. Conclusions: The collective characteristics of the internode regions were related to the varied recalcitrance found in the samples. Components such as lignin and GAX were critical for the increased recalcitrance, but low cellulose crystallinity index, high MLG contents, and highly substituted GAX contributed to the generation of a less recalcitrant material.},
doi = {10.1186/s13068-016-0513-2},
url = {https://www.osti.gov/biblio/1618639}, journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 9,
place = {Netherlands},
year = {Wed May 04 00:00:00 EDT 2016},
month = {Wed May 04 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1186/s13068-016-0513-2

Citation Metrics:
Cited by: 25 works
Citation information provided by
Web of Science

Figures / Tables:

Table 1 Table 1: Chemical composition of internode fractions from six different sugarcane hybrids

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Works referenced in this record:

Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis
journal, October 1999


(1,3;1,4)-β-D-Glucans in Cell Walls of the Poaceae, Lower Plants, and Fungi: A Tale of Two Linkages
journal, September 2009


Rhamnogalacturonan I in Solanum tuberosum tubers contains complex arabinogalactan structures
journal, May 2004


Chemical composition and enzymatic digestibility of sugarcane clones selected for varied lignin content
journal, January 2011


Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments
journal, January 2013


Hemicelluloses
journal, June 2010


Substrate factors that influence the synergistic interaction of AA9 and cellulases during the enzymatic hydrolysis of biomass
journal, January 2014


Maize Stem Tissues
journal, January 2006


Chemical composition and cell wall polysaccharide degradability of pith and rind tissues from mature maize internodes
journal, March 2012


How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010


Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property: Fig. 1.
journal, June 2015


Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis
journal, January 2010


Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose
journal, March 2012


Topochemical distribution of lignin and hydroxycinnamic acids in sugar-cane cell walls and its correlation with the enzymatic hydrolysis of polysaccharides
journal, January 2011


Abundance of mixed linkage glucan in mature tissues and secondary cell walls of grasses
journal, February 2013


Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007


Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010


Colorimetric Method for Determination of Sugars and Related Substances
journal, March 1956


Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass
journal, January 2013


ARABINAN DEFICIENT 1 Is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis
journal, December 2005


Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1)
journal, October 2011


Antibody-based screening of cell wall matrix glycans in ferns reveals taxon, tissue and cell-type specific distribution patterns
journal, January 2015


Chemical Composition and Enzymatic Degradability of Xylem and Nonxylem Walls Isolated from Alfalfa Internodes
journal, April 2002


(1->3),(1->4)- -d-Glucans in the cell walls of the Poales (sensu lato): an immunogold labeling study using a monoclonal antibody
journal, October 2005


Biosynthesis of Pectin
journal, April 2010


Cell Wall Development in Maize Coleoptiles
journal, September 1984


Cell Wall Architecture of the Elongating Maize Coleoptile
journal, October 2001


Changes in cell wall polysaccharides in developing barley (Hordeum vulgare) coleoptiles
journal, April 2005


The enzymatic recalcitrance of internodes of sugar cane hybrids with contrasting lignin contents
journal, November 2013


Unique aspects of the grass cell wall
journal, June 2008


Role of (1,3)(1,4)-β-Glucan in Cell Walls: Interaction with Cellulose
journal, April 2014


Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls
journal, January 2015


Works referencing / citing this record:

Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass
journal, July 2017


Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches
journal, December 2017


Comparative evaluation of acid and alkaline sulfite pretreatments for enzymatic saccharification of bagasses from three different sugarcane hybrids
journal, July 2018


Suberin and hemicellulose in sugarcane cell wall architecture and crop digestibility: A biotechnological perspective
journal, February 2019


Biodelignification and hydrolysis of rice straw by novel bacteria isolated from wood feeding termite
journal, October 2018


Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency
journal, October 2019


Sugarcane Cell Wall-Associated Defense Responses to Infection by Sporisorium scitamineum
journal, May 2018


Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.