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Title: Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Abstract

In this paper, we describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. Finally, we used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).

Authors:
 [1];  [2];  [1];  [1];  [3];  [3];  [4];  [3];  [5];  [3];  [3];  [3];  [6];  [6];  [6]; ORCiD logo [6];  [6];  [6];  [6];  [1] more »;  [1];  [7];  [3];  [1];  [5];  [3];  [3];  [8];  [7]; ORCiD logo [9] « less
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst.
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
  5. Humboldt Univ. of Berlin (Germany). Inst. of Biology
  6. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
  7. Stanford Univ., CA (United States). School of Medicine. Dept. of Structural Biology
  8. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource
  9. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Stanford Synchrotron Radiation Lightsource
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Laboratory Directed Research and Development (LDRD) Program; National Inst. of Health (NIH) (United States); Human Frontier Science Program (HFSP) (France)
Contributing Org.:
Humboldt Univ. of Berlin (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
OSTI Identifier:
1378710
Grant/Contract Number:  
AC02-05CH11231; AC02-76SF00515; P41GM103393; P41RR001209; GM51266; GM082545; GM055302; GM110501; GM095887; GM102520; RGP0005/2011; RGP0063/2013 310
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Methods
Additional Journal Information:
Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1548-7091
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microfluidics; molecular biophysics; nanocrystallography; proteins

Citation Formats

Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., and DeMirci, Hasan. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. United States: N. p., 2015. Web. doi:10.1038/nmeth.3667.
Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., & DeMirci, Hasan. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. United States. doi:10.1038/nmeth.3667.
Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., and DeMirci, Hasan. Mon . "Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II". United States. doi:10.1038/nmeth.3667. https://www.osti.gov/servlets/purl/1378710.
@article{osti_1378710,
title = {Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II},
author = {Sierra, Raymond G. and Gati, Cornelius and Laksmono, Hartawan and Dao, E. Han and Gul, Sheraz and Fuller, Franklin and Kern, Jan and Chatterjee, Ruchira and Ibrahim, Mohamed and Brewster, Aaron S. and Young, Iris D. and Michels-Clark, Tara and Aquila, Andrew and Liang, Mengning and Hunter, Mark S. and Koglin, Jason E. and Boutet, Sébastien and Junco, Elia A. and Hayes, Brandon and Bogan, Michael J. and Hampton, Christina Y. and Puglisi, Elisabetta V. and Sauter, Nicholas K. and Stan, Claudiu A. and Zouni, Athina and Yano, Junko and Yachandra, Vittal K. and Soltis, S. Michael and Puglisi, Joseph D. and DeMirci, Hasan},
abstractNote = {In this paper, we describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. Finally, we used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).},
doi = {10.1038/nmeth.3667},
journal = {Nature Methods},
number = 1,
volume = 13,
place = {United States},
year = {Mon Nov 30 00:00:00 EST 2015},
month = {Mon Nov 30 00:00:00 EST 2015}
}

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Works referenced in this record:

Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
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SEM imaging of liquid jets
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