A Versatile Microfluidic Device for Automating Synthetic Biology

Shih, Steve C.  C.; Goyal, Garima; Kim, Peter W.; Koutsoubelis, Nicolas; Keasling, Jay D.; Adams, Paul D.; Hillson, Nathan J.; Singh, Anup K.

doi:10.1021/acssynbio.5b00062

Title: A Versatile Microfluidic Device for Automating Synthetic Biology

Journal Article · Mon Jun 15 00:00:00 EDT 2015 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.5b00062· OSTI ID:1378593

Shih, Steve C. C. ^[1]; Goyal, Garima ^[2]; Kim, Peter W. ^[1]; Koutsoubelis, Nicolas ^[3]; Keasling, Jay D. ^[4]; Adams, Paul D. ^[5]; Hillson, Nathan J. ^[2]; Singh, Anup K. ^[1]

Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Technology Division; Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Technology Division; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Fuels Synthesis Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Fuels Synthesis Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Fuels Synthesis Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemical & Biomolecular Engineering, Dept. of Bioengineering
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Technology Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division

New microbes are being engineered that contain the genetic circuitry, metabolic pathways, and other cellular functions required for a wide range of applications such as producing biofuels, biobased chemicals, and pharmaceuticals. Although currently available tools are useful in improving the synthetic biology process, further improvements in physical automation would help to lower the barrier of entry into this field. We present an innovative microfluidic platform for assembling DNA fragments with 10× lower volumes (compared to that of current microfluidic platforms) and with integrated region-specific temperature control and on-chip transformation. Integration of these steps minimizes the loss of reagents and products compared to that with conventional methods, which require multiple pipetting steps. For assembling DNA fragments, we implemented three commonly used DNA assembly protocols on our microfluidic device: Golden Gate assembly, Gibson assembly, and yeast assembly (i.e., TAR cloning, DNA Assembler). Here, we demonstrate the utility of these methods by assembling two combinatorial libraries of 16 plasmids each. Each DNA plasmid is transformed into Escherichia coli or Saccharomyces cerevisiae using on-chip electroporation and further sequenced to verify the assembly. Lastly, we anticipate that this platform will enable new research that can integrate this automated microfluidic platform to generate large combinatorial libraries of plasmids and will help to expedite the overall synthetic biology process.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1378593

Journal Information:: ACS Synthetic Biology, Vol. 4, Issue 10; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 66 works

Citation information provided by
Web of Science

References (77)

Dispersing biofilms with engineered enzymatic bacteriophage Lu, T. K.; Collins, J. J. Proceedings of the National Academy of Sciences, Vol. 104, Issue 27 https://doi.org/10.1073/pnas.0704624104	journal	June 2007
Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy Lu, T. K.; Collins, J. J. Proceedings of the National Academy of Sciences, Vol. 106, Issue 12 https://doi.org/10.1073/pnas.0800442106	journal	March 2009
Industrial fermentation of renewable diesel fuels Westfall, Patrick J.; Gardner, Timothy S. Current Opinion in Biotechnology, Vol. 22, Issue 3 https://doi.org/10.1016/j.copbio.2011.04.023	journal	June 2011
Production of the antimalarial drug precursor artemisinic acid in engineered yeast Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario Nature, Vol. 440, Issue 7086, p. 940-943 https://doi.org/10.1038/nature04640	journal	April 2006
Advanced biofuel production in microbes Peralta-Yahya, Pamela P.; Keasling, Jay D. Biotechnology Journal, Vol. 5, Issue 2, p. 147-162 https://doi.org/10.1002/biot.200900220	journal	January 2010
Integration of microfluidics into the synthetic biology design flow Huang, Haiyao; Densmore, Douglas Lab Chip, Vol. 14, Issue 18 https://doi.org/10.1039/C4LC00509K	journal	January 2014
The Fusion of Biology, Computer Science, and Engineering: Towards Efficient and Successful Synthetic Biology Linshiz, Gregory; Goldberg, Alex; Konry, Tania Perspectives in Biology and Medicine, Vol. 55, Issue 4 https://doi.org/10.1353/pbm.2012.0044	journal	January 2012
Developer's and User's Guide to Clotho v2.0: A Software Platform for the Creation of Synthetic Biological Systems Xia, Bing; Bhatia, Swapnil; Bubenheim, Ben Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00005-X	book	January 2011
j5 DNA Assembly Design Automation Software Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D. ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21 https://doi.org/10.1021/sb2000116	journal	December 2011
GenoCAD for iGEM: a grammatical approach to the design of standard-compliant constructs Cai, Yizhi; Wilson, Mandy L.; Peccoud, Jean Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq086	journal	February 2010
Automatic Compilation from High-Level Biologically-Oriented Programming Language to Genetic Regulatory Networks Beal, Jacob; Lu, Ting; Weiss, Ron PLoS ONE, Vol. 6, Issue 8 https://doi.org/10.1371/journal.pone.0022490	journal	August 2011
Towards programming languages for genetic engineering of living cells Pedersen, Michael; Phillips, Andrew Journal of The Royal Society Interface, Vol. 6, Issue suppl_4 https://doi.org/10.1098/rsif.2008.0516.focus	journal	April 2009
Eugene – A Domain Specific Language for Specifying and Constraining Synthetic Biological Parts, Devices, and Systems Bilitchenko, Lesia; Liu, Adam; Cheung, Sherine PLoS ONE, Vol. 6, Issue 4 https://doi.org/10.1371/journal.pone.0018882	journal	April 2011
The Synthetic Biology Open Language (SBOL) provides a community standard for communicating designs in synthetic biology Galdzicki, Michal; Clancy, Kevin P.; Oberortner, Ernst Nature Biotechnology, Vol. 32, Issue 6 https://doi.org/10.1038/nbt.2891	journal	June 2014
Interactive assembly algorithms for molecular cloning Appleton, Evan; Tao, Jenhan; Haddock, Traci Nature Methods, Vol. 11, Issue 6 https://doi.org/10.1038/nmeth.2939	journal	April 2014
GeneDesign 3.0 is an updated synthetic biology toolkit Richardson, Sarah M.; Nunley, Paul W.; Yarrington, Robert M. Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq143	journal	March 2010
DeviceEditor visual biological CAD canvas Chen, Joanna; Densmore, Douglas; Ham, Timothy S. Journal of Biological Engineering, Vol. 6, Issue 1 https://doi.org/10.1186/1754-1611-6-1	journal	December 2012
PromoterCAD: data-driven design of plant regulatory DNA Cox, Robert Sidney; Nishikata, Koro; Shimoyama, Sayoko Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt518	journal	June 2013
Dynamic Modeling of Cellular Populations within iBioSim Stevens, Jason T.; Myers, Chris J. ACS Synthetic Biology, Vol. 2, Issue 5 https://doi.org/10.1021/sb300082b	journal	November 2012
XTMS: pathway design in an eXTended metabolic space Carbonell, Pablo; Parutto, Pierre; Herisson, Joan Nucleic Acids Research, Vol. 42, Issue W1 https://doi.org/10.1093/nar/gku362	journal	May 2014
DREAMS of metabolism Soh, Keng Cher; Hatzimanikatis, Vassily Trends in Biotechnology, Vol. 28, Issue 10 https://doi.org/10.1016/j.tibtech.2010.07.002	journal	October 2010
PR-PR: Cross-Platform Laboratory Automation System Linshiz, Gregory; Stawski, Nina; Goyal, Garima ACS Synthetic Biology, Vol. 3, Issue 8 https://doi.org/10.1021/sb4001728	journal	January 2014
A Low Cost, Customizable Turbidostat for Use in Synthetic Circuit Characterization Takahashi, Chris N.; Miller, Aaron W.; Ekness, Felix ACS Synthetic Biology, Vol. 4, Issue 1 https://doi.org/10.1021/sb500165g	journal	June 2014
PaR-PaR Laboratory Automation Platform Linshiz, Gregory; Stawski, Nina; Poust, Sean ACS Synthetic Biology, Vol. 2, Issue 5 https://doi.org/10.1021/sb300075t	journal	October 2012
A Computational Method for Automated Characterization of Genetic Components Yordanov, Boyan; Dalchau, Neil; Grant, Paul K. ACS Synthetic Biology, Vol. 3, Issue 8 https://doi.org/10.1021/sb400152n	journal	March 2014
Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan Nature Methods, Vol. 6, Issue 5, p. 343-345 https://doi.org/10.1038/nmeth.1318	journal	April 2009
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes Engler, Carola; Gruetzner, Ramona; Kandzia, Romy PLoS ONE, Vol. 4, Issue 5 https://doi.org/10.1371/journal.pone.0005553	journal	May 2009
A One Pot, One Step, Precision Cloning Method with High Throughput Capability Engler, Carola; Kandzia, Romy; Marillonnet, Sylvestre PLoS ONE, Vol. 3, Issue 11, Article No. e3647 https://doi.org/10.1371/journal.pone.0003647	journal	November 2008
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome Gibson, D. G.; Benders, G. A.; Axelrod, K. C. Proceedings of the National Academy of Sciences, Vol. 105, Issue 51 https://doi.org/10.1073/pnas.0811011106	journal	December 2008
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways Shao, Zengyi; Zhao, Hua; Zhao, Huimin Nucleic Acids Research, Vol. 37, Issue 2 https://doi.org/10.1093/nar/gkn991	journal	December 2008
Experimental and computational validation of models of fluorescent and luminescent reporter genes in bacteria de Jong, Hidde; Ranquet, Caroline; Ropers, Delphine BMC Systems Biology, Vol. 4, Issue 1 https://doi.org/10.1186/1752-0509-4-55	journal	January 2010
Algorithms for automated DNA assembly Densmore, Douglas; Hsiau, Timothy H. -C.; Kittleson, Joshua T. Nucleic Acids Research, Vol. 38, Issue 8 https://doi.org/10.1093/nar/gkq165	journal	March 2010
Automated Assembly of Standard Biological Parts Leguia, Mariana; Brophy, Jennifer; Densmore, Douglas Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00016-4	book	May 2011
Microfluidic devices for measuring gene network dynamics in single cells Bennett, Matthew R.; Hasty, Jeff Nature Reviews Genetics, Vol. 10, Issue 9 https://doi.org/10.1038/nrg2625	journal	August 2009
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips Kosuri, Sriram; Eroshenko, Nikolai; LeProust, Emily M. Nature Biotechnology, Vol. 28, Issue 12, p. 1295-1299 https://doi.org/10.1038/nbt.1716	journal	November 2010
Integrated two-step gene synthesis in a microfluidic device Huang, Mo Chao; Ye, Hongye; Kuan, Yoke Kong Lab Chip, Vol. 9, Issue 2 https://doi.org/10.1039/B807688J	journal	January 2009
Parallel gene synthesis in a microfluidic device Kong, D. S.; Carr, P. A.; Chen, L. Nucleic Acids Research, Vol. 35, Issue 8 https://doi.org/10.1093/nar/gkm121	journal	March 2007
Microfluidic cell arrays for metabolic monitoring of stimulated cardiomyocytes Cheng, Wei; Klauke, Norbert; Smith, Godfrey ELECTROPHORESIS, Vol. 31, Issue 8 https://doi.org/10.1002/elps.200900579	journal	April 2010
Guiding, distribution, and storage of trains of shape-dependent droplets Ahn, Byungwook; Lee, Kangsun; Lee, Hun Lab on a Chip, Vol. 11, Issue 22 https://doi.org/10.1039/c1lc20729f	journal	January 2011
Droplet Microfluidics-A Tool for Single-Cell Analysis Joensson, Haakan N.; Andersson Svahn, Helene Angewandte Chemie International Edition, Vol. 51, Issue 49 https://doi.org/10.1002/anie.201200460	journal	November 2012
Identification and genetic analysis of cancer cells with PCR-activated cell sorting Eastburn, Dennis J.; Sciambi, Adam; Abate, Adam R. Nucleic Acids Research, Vol. 42, Issue 16 https://doi.org/10.1093/nar/gku606	journal	July 2014
Generating electric fields in PDMS microfluidic devices with salt water electrodes Sciambi, Adam; Abate, Adam R. Lab Chip, Vol. 14, Issue 15 https://doi.org/10.1039/C4LC00078A	journal	January 2014
A modular approach for the generation, storage, mixing, and detection of droplet libraries for high throughput screening Trivedi, Varun; Doshi, Ankur; Kurup, G. K. Lab on a Chip, Vol. 10, Issue 18 https://doi.org/10.1039/c004768f	journal	January 2010
Automated Digital Microfluidic Platform for Magnetic-Particle-Based Immunoassays with Optimization by Design of Experiments Choi, Kihwan; Ng, Alphonsus H. C.; Fobel, Ryan Analytical Chemistry, Vol. 85, Issue 20 https://doi.org/10.1021/ac401847x	journal	August 2013
CHEMISTRY: Putting Electrowetting to Work Wheeler, A. R. Science, Vol. 322, Issue 5901 https://doi.org/10.1126/science.1165719	journal	October 2008
A droplet-based screen for wavelength-dependent lipid production in algae Shih, Steve C. C.; Mufti, Nooman S.; Chamberlain, M. Dean Energy & Environmental Science, Vol. 7, Issue 7 https://doi.org/10.1039/c4ee01123f	journal	January 2014
Multiplexed extraction and quantitative analysis of pharmaceuticals from DBS samples using digital microfluidics Lafrenière, Nelson M.; Shih, Steve CC; Abu-Rabie, Paul Bioanalysis, Vol. 6, Issue 3 https://doi.org/10.4155/bio.13.311	journal	February 2014
Digital Microfluidic Platform for the Detection of Rubella Infection and Immunity: A Proof of Concept Ng, Alphonsus H. C.; Lee, Misan; Choi, Kihwan Clinical Chemistry, Vol. 61, Issue 2 https://doi.org/10.1373/clinchem.2014.232181	journal	February 2015
Hybrid microfluidics: A digital-to-channel interface for in-line sample processing and chemical separations Abdelgawad, Mohamed; Watson, Michael W. L.; Wheeler, Aaron R. Lab on a Chip, Vol. 9, Issue 8 https://doi.org/10.1039/b820682a	journal	January 2009
Multilayer Hybrid Microfluidics: A Digital-to-Channel Interface for Sample Processing and Separations Watson, Michael W. L.; Jebrail, Mais J.; Wheeler, Aaron R. Analytical Chemistry, Vol. 82, Issue 15 https://doi.org/10.1021/ac101379g	journal	August 2010
An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR Rival, A.; Jary, D.; Delattre, C. Lab Chip, Vol. 14, Issue 19 https://doi.org/10.1039/C4LC00592A	journal	January 2014
A New Angle on Pluronic Additives: Advancing Droplets and Understanding in Digital Microfluidics Au, Sam H.; Kumar, Paresh; Wheeler, Aaron R. Langmuir, Vol. 27, Issue 13 https://doi.org/10.1021/la201185c	journal	July 2011
Single-cell analysis and sorting using droplet-based microfluidics Mazutis, Linas; Gilbert, John; Ung, W. Lloyd Nature Protocols, Vol. 8, Issue 5 https://doi.org/10.1038/nprot.2013.046	journal	April 2013
Genomic DNA Extraction from Cells by Electroporation on an Integrated Microfluidic Platform Geng, Tao; Bao, Ning; Sriranganathanw, Nammalwar Analytical Chemistry, Vol. 84, Issue 21 https://doi.org/10.1021/ac3026064	journal	September 2012
A single cell electroporation chip Khine, Michelle; Lau, Adrian; Ionescu-Zanetti, Cristian Lab on a Chip, Vol. 5, Issue 1 https://doi.org/10.1039/b408352k	journal	January 2005
Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal Qu, Baiyan; Eu, Young-Jae; Jeong, Won-Joong Lab on a Chip, Vol. 12, Issue 21 https://doi.org/10.1039/c2lc40360a	journal	January 2012
Digital microfluidics with impedance sensing for integrated cell culture andanalysis Shih, Steve C. C.; Barbulovic-Nad, Irena; Yang, Xuning Biosensors and Bioelectronics, Vol. 42 https://doi.org/10.1016/j.bios.2012.10.035	journal	April 2013
Core–Shell Droplets for Parallel DNA Ligation of an Ultra-micro Volume Using an EWOD Microfluidic System Lin, Hung-Chia; Liu, Yi-Ju; Yao, Da-Jeng Journal of the Association for Laboratory Automation, Vol. 15, Issue 3 https://doi.org/10.1016/j.jala.2010.01.010	journal	June 2010
DNA ligation of ultramicro volume using an EWOD microfluidic system with coplanar electrodes Liu, Yi-Ju; Yao, Da-Jeng; Lin, Hung-Chia Journal of Micromechanics and Microengineering, Vol. 18, Issue 4 https://doi.org/10.1088/0960-1317/18/4/045017	journal	March 2008
Integrated microbioreactor for culture and analysis of bacteria, algae and yeast Au, Sam H.; Shih, Steve C. C.; Wheeler, Aaron R. Biomedical Microdevices, Vol. 13, Issue 1 https://doi.org/10.1007/s10544-010-9469-3	journal	September 2010
Digital microfluidic chip technology for water permeability measurements on single isolated plant protoplasts Kumar, Phalguni Tewari; Toffalini, Federica; Witters, Daan Sensors and Actuators B: Chemical, Vol. 199 https://doi.org/10.1016/j.snb.2014.04.018	journal	August 2014
Circle-to-circle amplification on a digital microfluidic chip for amplified single molecule detection Kühnemund, Malte; Witters, Daan; Nilsson, Mats Lab Chip, Vol. 14, Issue 16 https://doi.org/10.1039/C4LC00348A	journal	January 2014
Programmable large area digital microfluidic array with integrated droplet sensing for bioassays Hadwen, B.; Broder, G. R.; Morganti, D. Lab on a Chip, Vol. 12, Issue 18 https://doi.org/10.1039/c2lc40273d	journal	January 2012
Toward active-matrix lab-on-a-chip: programmable electrofluidic control enabled by arrayed oxide thin film transistors Noh, Joo Hyon; Noh, Jiyong; Kreit, Eric Lab Chip, Vol. 12, Issue 2 https://doi.org/10.1039/C1LC20851A	journal	January 2012
Direct-Referencing Two-Dimensional-Array Digital Microfluidics Using Multilayer Printed Circuit Board Journal of Microelectromechanical Systems, Vol. 17, Issue 2 https://doi.org/10.1109/JMEMS.2007.912698	journal	April 2008
A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices Jebrail, Mais J.; Renzi, Ronald F.; Sinha, Anupama Lab on a Chip, Vol. 15, Issue 1 https://doi.org/10.1039/C4LC00703D	journal	January 2015
Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity Cho, Soongwon; Kang, Dong-Ku; Sim, Steven Analytical Chemistry, Vol. 85, Issue 18 https://doi.org/10.1021/ac4022067	journal	August 2013
Reliable microfluidic on-chip incubation of droplets in delay-lines Frenz, Lucas; Blank, Kerstin; Brouzes, Eric Lab Chip, Vol. 9, Issue 10 https://doi.org/10.1039/B816049J	journal	January 2009
Drop-based microfluidic devices for encapsulation of single cells Köster, Sarah; Angilè, Francesco E.; Duan, Honey Lab on a Chip, Vol. 8, Issue 7 https://doi.org/10.1039/b802941e	journal	January 2008
A Modular Cloning System for Standardized Assembly of Multigene Constructs Weber, Ernst; Engler, Carola; Gruetzner, Ramona PLoS ONE, Vol. 6, Issue 2 https://doi.org/10.1371/journal.pone.0016765	journal	February 2011
Droplet-based microfluidic platform for heterogeneous enzymatic assays Chang, Chieh; Sustarich, Jess; Bharadwaj, Rajiv Lab on a Chip, Vol. 13, Issue 9 https://doi.org/10.1039/c3lc41418c	journal	January 2013
Genetic programs constructed from layered logic gates in single cells Moon, Tae Seok; Lou, Chunbo; Tamsir, Alvin Nature, Vol. 491, Issue 7423 https://doi.org/10.1038/nature11516	journal	October 2012
Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies Goh, Ee-Been; Baidoo, Edward E. K.; Burd, Helcio Metabolic Engineering, Vol. 26 https://doi.org/10.1016/j.ymben.2014.09.003	journal	November 2014
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli Bokinsky, G.; Peralta-Yahya, P. P.; George, A. Proceedings of the National Academy of Sciences, Vol. 108, Issue 50, p. 19949-19954 https://doi.org/10.1073/pnas.1106958108	journal	November 2011
Sealing SU-8 microfluidic channels using PDMS Zhang, Zhiyi; Zhao, Ping; Xiao, Gaozhi Biomicrofluidics, Vol. 5, Issue 4 https://doi.org/10.1063/1.3659016	journal	December 2011
Dried Blood Spot Analysis by Digital Microfluidics Coupled to Nanoelectrospray Ionization Mass Spectrometry Shih, Steve C. C.; Yang, Hao; Jebrail, Mais J. Analytical Chemistry, Vol. 84, Issue 8, p. 3731-3738 https://doi.org/10.1021/ac300305s	journal	March 2012
Design, implementation and practice of JBEI-ICE: an open source biological part registry platform and tools Ham, T. S.; Dmytriv, Z.; Plahar, H. Nucleic Acids Research, Vol. 40, Issue 18 https://doi.org/10.1093/nar/gks531	journal	June 2012

Cited By (19)

Image-based feedback and analysis system for digital microfluidics Vo, Philippe Q. N.; Husser, Mathieu C.; Ahmadi, Fatemeh Lab on a Chip, Vol. 17, Issue 20 https://doi.org/10.1039/c7lc00826k	journal	January 2017
Microfluidics‐Implemented Biochemical Assays: From the Perspective of Readout Chen, Wenwen; Shao, Fangchi; Xianyu, Yunlei Small, Vol. 16, Issue 9 https://doi.org/10.1002/smll.201903388	journal	September 2019
End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis Linshiz, Gregory; Jensen, Erik; Stawski, Nina Journal of Biological Engineering, Vol. 10, Issue 1 https://doi.org/10.1186/s13036-016-0024-5	journal	February 2016
Scaling up genetic circuit design for cellular computing: advances and prospects Xiang, Yiyu; Dalchau, Neil; Wang, Baojun Natural Computing, Vol. 17, Issue 4 https://doi.org/10.1007/s11047-018-9715-9	journal	October 2018
Biofunctionalization of Glass‐ and Paper‐Based Microfluidic Devices: A Review Shakeri, Amid; Jarad, Noor Abu; Leung, Ashlyn Advanced Materials Interfaces, Vol. 6, Issue 19 https://doi.org/10.1002/admi.201900940	journal	August 2019
An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting Ahmadi, Fatemeh; Samlali, Kenza; Vo, Philippe Q. N. Lab on a Chip, Vol. 19, Issue 3 https://doi.org/10.1039/c8lc01170b	journal	January 2019
Common principles and best practices for engineering microbiomes Lawson, Christopher E.; Harcombe, William R.; Hatzenpichler, Roland Nature Reviews Microbiology, Vol. 17, Issue 12 https://doi.org/10.1038/s41579-019-0255-9	journal	September 2019
Why microfluidics? Merits and trends in chemical synthesis Liu, Yong; Jiang, Xingyu Lab on a Chip, Vol. 17, Issue 23 https://doi.org/10.1039/c7lc00627f	journal	January 2017
DNA assembly with error correction on a droplet digital microfluidics platform Khilko, Yuliya; Weyman, Philip D.; Glass, John I. BMC Biotechnology, Vol. 18, Issue 1 https://doi.org/10.1186/s12896-018-0439-9	journal	June 2018
RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes Abatemarco, Joseph; Sarhan, Maen F.; Wagner, James M. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00425-7	journal	August 2017
Standardization in synthetic biology: an engineering discipline coming of age Decoene, Thomas; De Paepe, Brecht; Maertens, Jo Critical Reviews in Biotechnology, Vol. 38, Issue 5 https://doi.org/10.1080/07388551.2017.1380600	journal	September 2017
Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable Kothamachu, Varun B.; Zaini, Sabrina; Muffatto, Federico SLAS TECHNOLOGY: Translating Life Sciences Innovation https://doi.org/10.1177/2472630320931794	journal	June 2020
Review of Microfluidic Photobioreactor Technology for Metabolic Engineering and Synthetic Biology of Cyanobacteria and Microalgae Yang, Ya-Tang; Wang, Chun Micromachines, Vol. 7, Issue 10 https://doi.org/10.3390/mi7100185	journal	October 2016
Digital microfluidic immobilized cytochrome P450 reactors with integrated inkjet-printed microheaters for droplet-based drug metabolism research Sathyanarayanan, Gowtham; Haapala, Markus; Kiiski, Iiro Analytical and Bioanalytical Chemistry, Vol. 410, Issue 25 https://doi.org/10.1007/s00216-018-1280-7	journal	August 2018
Synthetic and systems biology for microbial production of commodity chemicals Chubukov, Victor; Mukhopadhyay, Aindrila; Petzold, Christopher J. npj Systems Biology and Applications, Vol. 2, Issue 1 https://doi.org/10.1038/npjsba.2016.9	journal	April 2016
Microfabricated tools for quantitative plant biology Elitaş, Meltem; Yüce, Meral; Budak, Hikmet The Analyst, Vol. 142, Issue 6 https://doi.org/10.1039/c6an02643e	journal	January 2017
Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2) Yin, Jia; Hoffmann, Michael; Bian, Xiaoying Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep15081	journal	October 2015
One cell, one drop, one click: hybrid microfluidic mammalian single-cell isolation Samlali, Kenza; Ahmadi, Fatemeh; Quach, Angela B. V. Small https://doi.org/10.1101/2020.01.24.908202	posted_content	January 2020
Synthesis and cell-free cloning of DNA libraries using programmable microfluidics Yehezkel, Tuval Ben; Rival, Arnaud; Raz, Ofir Nucleic Acids Research, Vol. 44, Issue 4 https://doi.org/10.1093/nar/gkv1087	journal	October 2015

Similar Records

A Droplet Microfluidic Platform for Automating Genetic Engineering

Journal Article · Mon Feb 01 00:00:00 EST 2016 · ACS Synthetic Biology · OSTI ID:1378593

Gach, Philip C.; Shih, Steve C. C.; Sustarich, Jess; +4 more

Optimizing the violacein biosynthetic pathway using droplet microfluidics

Conference · Wed Jan 01 00:00:00 EST 2020 · OSTI ID:1378593

Gach, PC; Raje, M; Iwai, K; +7 more

End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

Journal Article · Tue Feb 02 00:00:00 EST 2016 · Journal of Biological Engineering · OSTI ID:1378593

Linshiz, Gregory; Jensen, Erik; Stawski, Nina; +7 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
digital microfluidics
DNA assembly
droplet microfluidics
Gibson assembly
Golden Gate assembly
synthetic biology
TAR cloning
yeast assembly

Title: A Versatile Microfluidic Device for Automating Synthetic Biology

Citation Formats

References (77)

Cited By (19)

Similar Records

Related Subjects