Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase
Abstract
Activated protein kinase R (PKR) plays a vital role in antiviral defense primarily by inhibiting protein synthesis and augmenting interferon responses. Many viral proteins have adopted unique strategies to counteract the deleterious effects of PKR. The NSs (Non-structural s) protein which is encoded by Rift Valley fever virus (RVFV) promotes early PKR proteasomal degradation through a previously undefined mechanism. In this study, we demonstrate that NSs carries out this activity by assembling the SCF (SKP1-CUL1-F-box)FBXW11 E3 ligase. NSs binds to the F-box protein, FBXW11, via the six amino acid sequence DDGFVE called the degron sequence and recruits PKR through an alternate binding site to the SCFFBXW11 E3 ligase. We further show that disrupting the assembly of the SCFFBXW11-NSs E3 ligase with MLN4924 (a small molecule inhibitor of SCF E3 ligase activity) or NSs degron viral mutants or siRNA knockdown of FBXW11 can block PKR degradation. Surprisingly, under these conditions when PKR degradation was blocked, NSs was essential and sufficient to activate PKR causing potent inhibition of RVFV infection by suppressing viral protein synthesis. These antiviral effects were antagonized by the loss of PKR expression or with a NSs deleted mutant virus. Therefore, early PKR activation by disassembly of SCFFBXW11-NSs E3more »
- Authors:
-
- United States Army Medical Research Inst. of Infectious Diseases, Frederick, MD (United States)
- Perkin Elmer, Waltham, MA (United States); United States Army Medical Research Inst. of Infectious Diseases, Frederick, MD (United States)
- Publication Date:
- Research Org.:
- United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland (United States); Defense Threat Reduction Agency (DTRA), Joint Science and Technology Office for Chem Bio Defense (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1378555
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- PLoS Pathogens
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 2; Journal ID: ISSN 1553-7374
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Mudhasani, Rajini, Tran, Julie P., Retterer, Cary, Kota, Krishna P., Whitehouse, Chris A., and Bavari, Sina. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. United States: N. p., 2016.
Web. doi:10.1371/journal.ppat.1005437.
Mudhasani, Rajini, Tran, Julie P., Retterer, Cary, Kota, Krishna P., Whitehouse, Chris A., & Bavari, Sina. Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase. United States. https://doi.org/10.1371/journal.ppat.1005437
Mudhasani, Rajini, Tran, Julie P., Retterer, Cary, Kota, Krishna P., Whitehouse, Chris A., and Bavari, Sina. 2016.
"Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase". United States. https://doi.org/10.1371/journal.ppat.1005437. https://www.osti.gov/servlets/purl/1378555.
@article{osti_1378555,
title = {Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase},
author = {Mudhasani, Rajini and Tran, Julie P. and Retterer, Cary and Kota, Krishna P. and Whitehouse, Chris A. and Bavari, Sina},
abstractNote = {Activated protein kinase R (PKR) plays a vital role in antiviral defense primarily by inhibiting protein synthesis and augmenting interferon responses. Many viral proteins have adopted unique strategies to counteract the deleterious effects of PKR. The NSs (Non-structural s) protein which is encoded by Rift Valley fever virus (RVFV) promotes early PKR proteasomal degradation through a previously undefined mechanism. In this study, we demonstrate that NSs carries out this activity by assembling the SCF (SKP1-CUL1-F-box)FBXW11 E3 ligase. NSs binds to the F-box protein, FBXW11, via the six amino acid sequence DDGFVE called the degron sequence and recruits PKR through an alternate binding site to the SCFFBXW11 E3 ligase. We further show that disrupting the assembly of the SCFFBXW11-NSs E3 ligase with MLN4924 (a small molecule inhibitor of SCF E3 ligase activity) or NSs degron viral mutants or siRNA knockdown of FBXW11 can block PKR degradation. Surprisingly, under these conditions when PKR degradation was blocked, NSs was essential and sufficient to activate PKR causing potent inhibition of RVFV infection by suppressing viral protein synthesis. These antiviral effects were antagonized by the loss of PKR expression or with a NSs deleted mutant virus. Therefore, early PKR activation by disassembly of SCFFBXW11-NSs E3 ligase is sufficient to inhibit RVFV infection. Furthermore, FBXW11 and BTRC are the two homologues of the βTrCP (Beta-transducin repeat containing protein) gene that were previously described to be functionally redundant. However, in RVFV infection, among the two homologues of βTrCP, FBXW11 plays a dominant role in PKR degradation and is the limiting factor in the assembly of the SCFFBXW11 complex. Thus, FBXW11 serves as a master regulator of RVFV infection by promoting PKR degradation. Overall these findings provide new insights into NSs regulation of PKR activity and offer potential opportunities for therapeutic intervention of RVFV infection.},
doi = {10.1371/journal.ppat.1005437},
url = {https://www.osti.gov/biblio/1378555},
journal = {PLoS Pathogens},
issn = {1553-7374},
number = 2,
volume = 12,
place = {United States},
year = {Tue Feb 02 00:00:00 EST 2016},
month = {Tue Feb 02 00:00:00 EST 2016}
}
Web of Science
Works referenced in this record:
Impaired degradation of inhibitory subunit of NF- B (I B) and -catenin as a result of targeted disruption of the -TrCP1 gene
journal, July 2003
- Nakayama, K.; Hatakeyama, S.; Maruyama, S. -i.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 15
The CUL1 C-Terminal Sequence and ROC1 Are Required for Efficient Nuclear Accumulation, NEDD8 Modification, and Ubiquitin Ligase Activity of CUL1
journal, November 2000
- Furukawa, Manabu; Zhang, Yanping; McCarville, Joseph
- Molecular and Cellular Biology, Vol. 20, Issue 21
Specificity, location and function of βTrCP isoforms and their splice variants
journal, April 2011
- Putters, Joyce; Slotman, Johan A.; Gerlach, Jan P.
- Cellular Signalling, Vol. 23, Issue 4
Acid-Activated Structural Reorganization of the Rift Valley Fever Virus Gc Fusion Protein
journal, October 2012
- de Boer, S. M.; Kortekaas, J.; Spel, L.
- Journal of Virology, Vol. 86, Issue 24
Role of the cytosolic tails of Rift Valley fever virus envelope glycoproteins in viral morphogenesis
journal, January 2014
- Carnec, Xavier; Ermonval, Myriam; Kreher, Felix
- Virology, Vol. 448
A Dominant-negative UBC12 Mutant Sequesters NEDD8 and Inhibits NEDD8 Conjugation in Vivo
journal, May 2000
- Wada, Hiroyoshi; Yeh, Edward T. H.; Kamitani, Tetsu
- Journal of Biological Chemistry, Vol. 275, Issue 22
Function and regulation of cullin–RING ubiquitin ligases
journal, January 2005
- Petroski, Matthew D.; Deshaies, Raymond J.
- Nature Reviews Molecular Cell Biology, Vol. 6, Issue 1
Development of High-Content Imaging Assays for Lethal Viral Pathogens
journal, August 2010
- Panchal, Rekha G.; Kota, Krishna P.; Spurgers, Kevin B.
- Journal of Biomolecular Screening, Vol. 15, Issue 7
DEPTOR, an mTOR Inhibitor, Is a Physiological Substrate of SCFβTrCP E3 Ubiquitin Ligase and Regulates Survival and Autophagy
journal, October 2011
- Zhao, Yongchao; Xiong, Xiufang; Sun, Yi
- Molecular Cell, Vol. 44, Issue 2
A ΩX a V motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence
journal, April 2015
- Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 19
Nedd8 on cullin: building an expressway to protein destruction
journal, March 2004
- Pan, Zhen-Qiang; Kentsis, Alex; Dias, Dora C.
- Oncogene, Vol. 23, Issue 11
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
journal, December 2001
- Livak, Kenneth J.; Schmittgen, Thomas D.
- Methods, Vol. 25, Issue 4
High-Content Image–Based Screening of a Signal Transduction Pathway Inhibitor Small-Molecule Library against Highly Pathogenic RNA Viruses
journal, January 2015
- Mudhasani, Rajini; Kota, Krishna P.; Retterer, Cary
- Journal of Biomolecular Screening, Vol. 20, Issue 1
Identification of Substrates for F‐Box Proteins
book, December 2005
- Jin, Jianping; Ang, Xiaolu L.; Shirogane, Takahiro
- Methods in Enzymology
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase
journal, April 2008
- Hu, J.; Zacharek, S.; He, Y. J.
- Genes & Development, Vol. 22, Issue 7
Double-Stranded RNA Is Produced by Positive-Strand RNA Viruses and DNA Viruses but Not in Detectable Amounts by Negative-Strand RNA Viruses
journal, May 2006
- Weber, Friedemann; Wagner, Valentina; Rasmussen, Simon B.
- Journal of Virology, Vol. 80, Issue 10
The many faces of β-TrCP E3 ubiquitin ligases: reflections in the magic mirror of cancer
journal, March 2004
- Fuchs, Serge Y.; Spiegelman, Vladimir S.; Suresh Kumar, K. G.
- Oncogene, Vol. 23, Issue 11
Tipping the Balance: Antagonism of PKR Kinase and ADAR1 Deaminase Functions by Virus Gene Products
journal, September 2009
- George, Cyril X.; Li, Zhiqun; Okonski, Kristina M.
- Journal of Interferon & Cytokine Research, Vol. 29, Issue 9
High Content Image-Based Screening of a Protease Inhibitor Library Reveals Compounds Broadly Active against Rift Valley Fever Virus and Other Highly Pathogenic RNA Viruses
journal, August 2014
- Mudhasani, Rajini; Kota, Krishna P.; Retterer, Cary
- PLoS Neglected Tropical Diseases, Vol. 8, Issue 8
Homodimer of Two F-box Proteins βTrCP1 or βTrCP2 Binds to IκBα for Signal-dependent Ubiquitination
journal, January 2000
- Suzuki, Hiroshi; Chiba, Tomoki; Suzuki, Toshiaki
- Journal of Biological Chemistry, Vol. 275, Issue 4
The cytoplasmic tail of hantavirus Gn glycoprotein interacts with RNA
journal, September 2011
- Strandin, Tomas; Hepojoki, Jussi; Wang, Hao
- Virology, Vol. 418, Issue 1
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function
journal, March 2006
- Andrews, P.; He, Y. J.; Xiong, Y.
- Oncogene, Vol. 25, Issue 33
Deregulated proteolysis by the F-box proteins SKP2 and β-TrCP: tipping the scales of cancer
journal, June 2008
- Frescas, David; Pagano, Michele
- Nature Reviews Cancer, Vol. 8, Issue 6
Rift Valley Fever Virus NSs Protein Promotes Post-Transcriptional Downregulation of Protein Kinase PKR and Inhibits eIF2α Phosphorylation
journal, February 2009
- Ikegami, Tetsuro; Narayanan, Krishna; Won, Sungyong
- PLoS Pathogens, Vol. 5, Issue 2
Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells
journal, January 2014
- Narayanan, Aarthi; Amaya, Moushimi; Voss, Kelsey
- Virology, Vol. 449
Rift Valley fever virus NSs inhibits host transcription independently of the degradation of dsRNA-dependent protein kinase PKR
journal, January 2013
- Kalveram, Birte; Lihoradova, Olga; Indran, Sabarish V.
- Virology, Vol. 435, Issue 2
Characterization of Clone 13, a Naturally Attenuated Avirulent Isolate of Rift Valley Fever Virus, which is Altered in the Small Segment *
journal, October 1995
- Turell, Michael; Saluzzo, Jean-Francois; Dreier, Thomas
- The American Journal of Tropical Medicine and Hygiene, Vol. 53, Issue 4
Critical role for IL-1β in DNA damage-induced mucositis
journal, January 2014
- Kanarek, Naama; Grivennikov, Sergei I.; Leshets, Michael
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 6
Evaluation of the efficacy and safety of the Rift Valley Fever Clone 13 vaccine in sheep
journal, June 2010
- Dungu, Baptiste; Louw, Ian; Lubisi, Alison
- Vaccine, Vol. 28, Issue 29
Rapid Accumulation of Virulent Rift Valley Fever Virus in Mice from an Attenuated Virus Carrying a Single Nucleotide Substitution in the M RNA
journal, April 2010
- Morrill, John C.; Ikegami, Tetsuro; Yoshikawa-Iwata, Naoko
- PLoS ONE, Vol. 5, Issue 4
Systematic analysis and nomenclature of mammalian F-box proteins
journal, November 2004
- Jin, J.
- Genes & Development, Vol. 18, Issue 21
An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer
journal, April 2009
- Soucy, Teresa A.; Smith, Peter G.; Milhollen, Michael A.
- Nature, Vol. 458, Issue 7239
The CUL1 C-Terminal Sequence and ROC1 Are Required for Efficient Nuclear Accumulation, NEDD8 Modification, and Ubiquitin Ligase Activity of CUL1
journal, January 2000
- Furukawa, Manabu; Zhang, Yanping; McCarville, Joseph
- Molecular and Cellular Biology, Vol. 20, Issue 21
Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase
text, January 2015
- B., Major, Michael; Dennis, Goldfarb,; Young, Kim, Tai
- The University of North Carolina at Chapel Hill University Libraries
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase
text, January 2008
- S., Shumway,; J., He, Y.; S., Zacharek,
- The University of North Carolina at Chapel Hill University Libraries
NEDD8 Modification of CUL1 Dissociates p120CAND1, an Inhibitor of CUL1-SKP1 Binding and SCF Ligases
text, January 2002
- Tomohiro, Matsumoto,; Manabu, Furukawa,; Jidong, Liu,
- The University of North Carolina at Chapel Hill University Libraries
ROC1, a Homolog of APC11, Represents a Family of Cullin Partners with an Associated Ubiquitin Ligase Activity
text, January 1999
- J., Schottelius, Arndt; Yue, Xiong,; Tomohiko, Ohta,
- The University of North Carolina at Chapel Hill University Libraries
Specificity, location and function of βTrCP isoforms and their splice variants
journal, April 2011
- Putters, Joyce; Slotman, Johan A.; Gerlach, Jan P.
- Cellular Signalling, Vol. 23, Issue 4
DEPTOR, an mTOR Inhibitor, Is a Physiological Substrate of SCFβTrCP E3 Ubiquitin Ligase and Regulates Survival and Autophagy
journal, October 2011
- Zhao, Yongchao; Xiong, Xiufang; Sun, Yi
- Molecular Cell, Vol. 44, Issue 2
Evaluation of the efficacy and safety of the Rift Valley Fever Clone 13 vaccine in sheep
journal, June 2010
- Dungu, Baptiste; Louw, Ian; Lubisi, Alison
- Vaccine, Vol. 28, Issue 29
The cytoplasmic tail of hantavirus Gn glycoprotein interacts with RNA
journal, September 2011
- Strandin, Tomas; Hepojoki, Jussi; Wang, Hao
- Virology, Vol. 418, Issue 1
Rift Valley fever virus NSs inhibits host transcription independently of the degradation of dsRNA-dependent protein kinase PKR
journal, January 2013
- Kalveram, Birte; Lihoradova, Olga; Indran, Sabarish V.
- Virology, Vol. 435, Issue 2
Role of the cytosolic tails of Rift Valley fever virus envelope glycoproteins in viral morphogenesis
journal, January 2014
- Carnec, Xavier; Ermonval, Myriam; Kreher, Felix
- Virology, Vol. 448
ROC1, a Homolog of APC11, Represents a Family of Cullin Partners with an Associated Ubiquitin Ligase Activity
journal, April 1999
- Ohta, Tomohiko; Michel, Jennifer J.; Schottelius, Arndt J.
- Molecular Cell, Vol. 3, Issue 4
NEDD8 Modification of CUL1 Dissociates p120CAND1, an Inhibitor of CUL1-SKP1 Binding and SCF Ligases
journal, December 2002
- Liu, Jidong; Furukawa, Manabu; Matsumoto, Tomohiro
- Molecular Cell, Vol. 10, Issue 6
Control of Meiotic and Mitotic Progression by the F Box Protein β-Trcp1 In Vivo
journal, June 2003
- Guardavaccaro, Daniele; Kudo, Yasusei; Boulaire, Jérôme
- Developmental Cell, Vol. 4, Issue 6
An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer
journal, April 2009
- Soucy, Teresa A.; Smith, Peter G.; Milhollen, Michael A.
- Nature, Vol. 458, Issue 7239
Deregulated proteolysis by the F-box proteins SKP2 and β-TrCP: tipping the scales of cancer
journal, June 2008
- Frescas, David; Pagano, Michele
- Nature Reviews Cancer, Vol. 8, Issue 6
Function and regulation of cullin–RING ubiquitin ligases
journal, January 2005
- Petroski, Matthew D.; Deshaies, Raymond J.
- Nature Reviews Molecular Cell Biology, Vol. 6, Issue 1
The many faces of β-TrCP E3 ubiquitin ligases: reflections in the magic mirror of cancer
journal, March 2004
- Fuchs, Serge Y.; Spiegelman, Vladimir S.; Suresh Kumar, K. G.
- Oncogene, Vol. 23, Issue 11
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function
journal, March 2006
- Andrews, P.; He, Y. J.; Xiong, Y.
- Oncogene, Vol. 25, Issue 33
Impaired degradation of inhibitory subunit of NF- B (I B) and -catenin as a result of targeted disruption of the -TrCP1 gene
journal, July 2003
- Nakayama, K.; Hatakeyama, S.; Maruyama, S. -i.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 15
A ΩX a V motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence
journal, April 2015
- Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 19
A Dominant-negative UBC12 Mutant Sequesters NEDD8 and Inhibits NEDD8 Conjugation in Vivo
journal, May 2000
- Wada, Hiroyoshi; Yeh, Edward T. H.; Kamitani, Tetsu
- Journal of Biological Chemistry, Vol. 275, Issue 22
Induction of DNA Damage Signaling upon Rift Valley Fever Virus Infection Results in Cell Cycle Arrest and Increased Viral Replication
journal, January 2012
- Baer, Alan; Austin, Dana; Narayanan, Aarthi
- Journal of Biological Chemistry, Vol. 287, Issue 10
Tipping the Balance: Antagonism of PKR Kinase and ADAR1 Deaminase Functions by Virus Gene Products
journal, September 2009
- George, Cyril X.; Li, Zhiqun; Okonski, Kristina M.
- Journal of Interferon & Cytokine Research, Vol. 29, Issue 9
Systematic analysis and nomenclature of mammalian F-box proteins
journal, November 2004
- Jin, J.
- Genes & Development, Vol. 18, Issue 21
The CUL1 C-Terminal Sequence and ROC1 Are Required for Efficient Nuclear Accumulation, NEDD8 Modification, and Ubiquitin Ligase Activity of CUL1
journal, January 2000
- Furukawa, Manabu; Zhang, Yanping; McCarville, Joseph
- Molecular and Cellular Biology, Vol. 20, Issue 21
Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR
journal, July 2002
- Drosten, C.; Gottig, S.; Schilling, S.
- Journal of Clinical Microbiology, Vol. 40, Issue 7
Role of the Cytoplasmic Tail Domains of Bunyamwera Orthobunyavirus Glycoproteins Gn and Gc in Virus Assembly and Morphogenesis
journal, July 2007
- Shi, Xiaohong; Kohl, Alain; Li, Ping
- Journal of Virology, Vol. 81, Issue 18
Loss of Protein Kinase PKR Expression in Human HeLa Cells Complements the Vaccinia Virus E3L Deletion Mutant Phenotype by Restoration of Viral Protein Synthesis
journal, October 2007
- Zhang, Ping; Jacobs, Bertram L.; Samuel, Charles E.
- Journal of Virology, Vol. 82, Issue 2
NSs Protein of Rift Valley Fever Virus Induces the Specific Degradation of the Double-Stranded RNA-Dependent Protein Kinase
journal, February 2009
- Habjan, Matthias; Pichlmair, Andreas; Elliott, Richard M.
- Journal of Virology, Vol. 83, Issue 9
NSs Protein of Rift Valley Fever Virus Promotes Posttranslational Downregulation of the TFIIH Subunit p62
journal, May 2011
- Kalveram, B.; Lihoradova, O.; Ikegami, T.
- Journal of Virology, Vol. 85, Issue 13
Virulence Factor NSs of Rift Valley Fever Virus Recruits the F-Box Protein FBXO3 To Degrade Subunit p62 of General Transcription Factor TFIIH
journal, January 2014
- Kainulainen, M.; Habjan, M.; Hubel, P.
- Journal of Virology, Vol. 88, Issue 6
IFITM-2 and IFITM-3 but Not IFITM-1 Restrict Rift Valley Fever Virus
journal, May 2013
- Mudhasani, R.; Tran, J. P.; Retterer, C.
- Journal of Virology, Vol. 87, Issue 15
The cellular 68,000-Mr protein kinase is highly autophosphorylated and activated yet significantly degraded during poliovirus infection: implications for translational regulation.
journal, January 1989
- Black, T. L.; Safer, B.; Hovanessian, A.
- Journal of Virology, Vol. 63, Issue 5
Genetic Evidence for an Interferon-Antagonistic Function of Rift Valley Fever Virus Nonstructural Protein NSs
journal, February 2001
- Bouloy, Michèle; Janzen, Christian; Vialat, Pierre
- Journal of Virology, Vol. 75, Issue 3
Rescue of Infectious Rift Valley Fever Virus Entirely from cDNA, Analysis of Virus Lacking the NSs Gene, and Expression of a Foreign Gene
journal, February 2006
- Ikegami, T.; Won, S.; Peters, C. J.
- Journal of Virology, Vol. 80, Issue 6
Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase
journal, October 2014
- Kim, Tai Young; Siesser, Priscila F.; Rossman, Kent L.
- Molecular and Cellular Biology, Vol. 35, Issue 1
High-Content Image–Based Screening of a Signal Transduction Pathway Inhibitor Small-Molecule Library against Highly Pathogenic RNA Viruses
journal, January 2015
- Mudhasani, Rajini; Kota, Krishna P.; Retterer, Cary
- Journal of Biomolecular Screening, Vol. 20, Issue 1
Transcriptome and de novo analysis of Rosa xanthina f. spontanea in response to cold stress
journal, October 2021
- Zhuang, Defeng; Ma, Ce; Xue, Li
- BMC Plant Biology, Vol. 21, Issue 1
Rapid Accumulation of Virulent Rift Valley Fever Virus in Mice from an Attenuated Virus Carrying a Single Nucleotide Substitution in the M RNA
journal, April 2010
- Morrill, John C.; Ikegami, Tetsuro; Yoshikawa-Iwata, Naoko
- PLoS ONE, Vol. 5, Issue 4
Rift Valley Fever Virus NSs Protein Promotes Post-Transcriptional Downregulation of Protein Kinase PKR and Inhibits eIF2α Phosphorylation
journal, February 2009
- Ikegami, Tetsuro; Narayanan, Krishna; Won, Sungyong
- PLoS Pathogens, Vol. 5, Issue 2
Works referencing / citing this record:
Viral proteins targeting host protein kinase R to evade an innate immune response: a mini review
journal, January 2018
- Dzananovic, Edis; McKenna, Sean A.; Patel, Trushar R.
- Biotechnology and Genetic Engineering Reviews, Vol. 34, Issue 1
Translational Control in Virus-Infected Cells
journal, June 2018
- Stern-Ginossar, Noam; Thompson, Sunnie R.; Mathews, Michael B.
- Cold Spring Harbor Perspectives in Biology, Vol. 11, Issue 3
NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and β-TRCP1 To Degrade the Antiviral Protein Kinase PKR
journal, April 2016
- Kainulainen, Markus; Lau, Simone; Samuel, Charles E.
- Journal of Virology, Vol. 90, Issue 13
NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3
journal, September 2018
- Wuerth, Jennifer Deborah; Habjan, Matthias; Wulle, Julia
- Journal of Virology, Vol. 92, Issue 23
Rift Valley fever virus NSs protein functions and the similarity to other bunyavirus NSs proteins
journal, July 2016
- Ly, Hoai J.; Ikegami, Tetsuro
- Virology Journal, Vol. 13, Issue 1
Rescue of infectious Arumowot virus from cloned cDNA: Posttranslational degradation of Arumowot virus NSs protein in human cells
journal, November 2019
- Hallam, Hoai J.; Lokugamage, Nandadeva; Ikegami, Tetsuro
- PLOS Neglected Tropical Diseases, Vol. 13, Issue 11
Toscana virus non-structural protein NSs acts as E3 ubiquitin ligase promoting RIG-I degradation
journal, December 2019
- Gori Savellini, Gianni; Anichini, Gabriele; Gandolfo, Claudia
- PLOS Pathogens, Vol. 15, Issue 12
Cellular Cullin RING Ubiquitin Ligases: Druggable Host Dependency Factors of Cytomegaloviruses
journal, April 2019
- Becker, Tanja; Le-Trilling, Vu; Trilling, Mirko
- International Journal of Molecular Sciences, Vol. 20, Issue 7
Phleboviruses and the Type I Interferon Response
journal, June 2016
- Wuerth, Jennifer; Weber, Friedemann
- Viruses, Vol. 8, Issue 6
Alterations in the host transcriptome in vitro following Rift Valley fever virus infection
journal, October 2017
- Pinkham, Chelsea; Dahal, Bibha; de la Fuente, Cynthia L.
- Scientific Reports, Vol. 7, Issue 1
A single-cycle replicable Rift Valley fever phlebovirus vaccine carrying a mutated NSs confers full protection from lethal challenge in mice
journal, November 2018
- Terasaki, Kaori; Juelich, Terry L.; Smith, Jennifer K.
- Scientific Reports, Vol. 8, Issue 1
VHS, US3 and UL13 viral tegument proteins are required for Herpes Simplex Virus-Induced modification of protein kinase R
journal, March 2020
- Pennisi, Rosamaria; Musarra-Pizzo, Maria; Lei, Zhixiang
- Scientific Reports, Vol. 10, Issue 1
Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR)
journal, March 2019
- Goodman, Danielle E.; Pretto, Carla D.; Krepostman, Tomas A.
- mBio
NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and β-TRCP1 To Degrade the Antiviral Protein Kinase PKR
journal, April 2016
- Kainulainen, Markus; Lau, Simone; Samuel, Charles E.
- Journal of Virology, Vol. 90, Issue 13
NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3
journal, September 2018
- Wuerth, Jennifer Deborah; Habjan, Matthias; Wulle, Julia
- Journal of Virology, Vol. 92, Issue 23
Mechanistic Insight into the Host Transcription Inhibition Function of Rift Valley Fever Virus NSs and Its Importance in Virulence
journal, October 2016
- Terasaki, Kaori; Ramirez, Sydney I.; Makino, Shinji
- PLOS Neglected Tropical Diseases, Vol. 10, Issue 10
Toscana virus non-structural protein NSs acts as E3 ubiquitin ligase promoting RIG-I degradation
journal, December 2019
- Gori Savellini, Gianni; Anichini, Gabriele; Gandolfo, Claudia
- PLOS Pathogens, Vol. 15, Issue 12
Cellular Cullin RING Ubiquitin Ligases: Druggable Host Dependency Factors of Cytomegaloviruses
journal, April 2019
- Becker, Tanja; Le-Trilling, Vu; Trilling, Mirko
- International Journal of Molecular Sciences, Vol. 20, Issue 7
Phleboviruses and the Type I Interferon Response
journal, June 2016
- Wuerth, Jennifer; Weber, Friedemann
- Viruses, Vol. 8, Issue 6
Characterization of the non-structural (NSs) protein of tick-borne phleboviruses
text, January 2017
- Rezelj, Veronica Valentina
- University of Glasgow