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Variants of glycerol dehydrogenase having D-lactate dehydrogenase activity and uses thereof

Patent ·
OSTI ID:1378093
; ;

The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.

Research Organization:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG36-04GO14019; FG36-08GO88142
Assignee:
UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
Patent Number(s):
9,745,556
Application Number:
14/347,688
OSTI ID:
1378093
Country of Publication:
United States
Language:
English

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Lactic acid production by Bacillus coagulans—kinetic studies and optimization of culture medium for batch and continuous fermentations journal December 1998
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Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12 journal March 2008
Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose journal November 2011

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