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Title: Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs

Abstract

Simultaneous detection and imaging of multiple intracellular messenger RNA (mRNAs) hold great significant for early cancer diagnostics and preventive medicine development. Herein, we propose a multiple-targeted graphene oxide (GO) nanocarrier that can simultaneously detect and image different type mRNAs in living cells. First of all, in vitro detection of multiple targets have been realized successfully based on the multiple-targeted GO nanocarrier with linear relationship ranging from 3 nM to 200 nM, as well as sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA. Additionally, this nanosensing platform composed of fluorescent labeled single strand DNA probes and GO nanocarrier can identify Mn-SOD mRNA and endogenous mRNA of β-actin in living cancer cells, showing rapid response, high specificity, nuclease stability, and good biocompatibility during the cell imaging. Thirdly, changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully. By using multiple ssDNA as probes and GO nanocarrier as the cellular delivery cargo, the proposed simultaneous multiple-targeted sensing platform will be of great potential as a powerful tool for intracellular trafficking process from basic research to clinical diagnosis.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1378036
Report Number(s):
PNNL-SA-126440
Journal ID: ISSN 0003-2670; 39985; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytica Chimica Acta
Additional Journal Information:
Journal Volume: 986; Journal Issue: 2017; Journal ID: ISSN 0003-2670
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; mRNA; graphene oxide; living cell; imaging; multiple detection; Environmental Molecular Sciences Laboratory

Citation Formats

Wang, Ying, Li, Zhaohui, Liu, Misha, Hu, Dehong, Lin, Yuehe, and Li, Jinghong. Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs. United States: N. p., 2017. Web. doi:10.1016/j.aca.2017.07.017.
Wang, Ying, Li, Zhaohui, Liu, Misha, Hu, Dehong, Lin, Yuehe, & Li, Jinghong. Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs. United States. doi:10.1016/j.aca.2017.07.017.
Wang, Ying, Li, Zhaohui, Liu, Misha, Hu, Dehong, Lin, Yuehe, and Li, Jinghong. Tue . "Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs". United States. doi:10.1016/j.aca.2017.07.017.
@article{osti_1378036,
title = {Multiple-Targeted Graphene-based Nanocarrier for Intracellular Imaging of mRNAs},
author = {Wang, Ying and Li, Zhaohui and Liu, Misha and Hu, Dehong and Lin, Yuehe and Li, Jinghong},
abstractNote = {Simultaneous detection and imaging of multiple intracellular messenger RNA (mRNAs) hold great significant for early cancer diagnostics and preventive medicine development. Herein, we propose a multiple-targeted graphene oxide (GO) nanocarrier that can simultaneously detect and image different type mRNAs in living cells. First of all, in vitro detection of multiple targets have been realized successfully based on the multiple-targeted GO nanocarrier with linear relationship ranging from 3 nM to 200 nM, as well as sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA. Additionally, this nanosensing platform composed of fluorescent labeled single strand DNA probes and GO nanocarrier can identify Mn-SOD mRNA and endogenous mRNA of β-actin in living cancer cells, showing rapid response, high specificity, nuclease stability, and good biocompatibility during the cell imaging. Thirdly, changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully. By using multiple ssDNA as probes and GO nanocarrier as the cellular delivery cargo, the proposed simultaneous multiple-targeted sensing platform will be of great potential as a powerful tool for intracellular trafficking process from basic research to clinical diagnosis.},
doi = {10.1016/j.aca.2017.07.017},
journal = {Analytica Chimica Acta},
issn = {0003-2670},
number = 2017,
volume = 986,
place = {United States},
year = {2017},
month = {8}
}