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Title: Expression of enzymes in yeast for lignocellulose derived oligomer CBP

Abstract

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Inventors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Lallemand Hungary Liquidity Management LLC, Budapest, HU (Hungary)
Sponsoring Org.:
USDOE
OSTI Identifier:
1377880
Patent Number(s):
9,745,560
Application Number:
14/424,560
Assignee:
Lallemand Hungary Liquidity Management LLC GFO
DOE Contract Number:  
FC36-08GO18103
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Mar 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., and Stonehouse, Emily. Expression of enzymes in yeast for lignocellulose derived oligomer CBP. United States: N. p., 2017. Web.
McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., & Stonehouse, Emily. Expression of enzymes in yeast for lignocellulose derived oligomer CBP. United States.
McBride, John E., Wiswall, Erin, Shikhare, Indraneel, Xu, Haowen, Thorngren, Naomi, Hau, Heidi H., and Stonehouse, Emily. Tue . "Expression of enzymes in yeast for lignocellulose derived oligomer CBP". United States. doi:. https://www.osti.gov/servlets/purl/1377880.
@article{osti_1377880,
title = {Expression of enzymes in yeast for lignocellulose derived oligomer CBP},
author = {McBride, John E. and Wiswall, Erin and Shikhare, Indraneel and Xu, Haowen and Thorngren, Naomi and Hau, Heidi H. and Stonehouse, Emily},
abstractNote = {The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 29 00:00:00 EDT 2017},
month = {Tue Aug 29 00:00:00 EDT 2017}
}

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