Expression of enzymes in yeast for lignocellulose derived oligomer CBP

McBride, John E.; Wiswall, Erin; Shikhare, Indraneel; Xu, Haowen; Thorngren, Naomi; Hau, Heidi H.; Stonehouse, Emily

Expression of enzymes in yeast for lignocellulose derived oligomer CBP

Patent · 29 August 2017

OSTI ID:1377880

McBride, John E.; Wiswall, Erin; Shikhare, Indraneel; Xu, Haowen; Thorngren, Naomi; Hau, Heidi H.; Stonehouse, Emily

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

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Research Organization:: Lallemand Hungary Liquidity Management LLC, Budapest, HU (Hungary)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC36-08GO18103;

Assignee:: Lallemand Hungary Liquidity Management LLC

Patent Number(s):: 9,745,560

Application Number:: 14/424,560

OSTI ID:: 1377880

Country of Publication:: United States

Language:: English

References (21)

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Expression of enzymes in yeast for lignocellulose derived oligomer CBP

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