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Title: Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)

Abstract

The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).

Inventors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1377879
Patent Number(s):
9,745,587
Application Number:
13/088,288
Assignee:
The Regents of the University of California LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Apr 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gilmore, Joshua M., Anderson, J. Christopher, and Dueber, John E. Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs). United States: N. p., 2017. Web.
Gilmore, Joshua M., Anderson, J. Christopher, & Dueber, John E. Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs). United States.
Gilmore, Joshua M., Anderson, J. Christopher, and Dueber, John E. Tue . "Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)". United States. https://www.osti.gov/servlets/purl/1377879.
@article{osti_1377879,
title = {Nucleic acid constructs containing orthogonal site selective recombinases (OSSRs)},
author = {Gilmore, Joshua M. and Anderson, J. Christopher and Dueber, John E.},
abstractNote = {The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {8}
}

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