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Title: Methods and compositions for controlling gene expression by RNA processing

Abstract

The present disclosure provides nucleic acids encoding an RNA recognition sequence positioned proximal to an insertion site for the insertion of a sequence of interest; and host cells genetically modified with the nucleic acids. The present disclosure also provides methods of modifying the activity of a target RNA, and kits and compositions for carrying out the methods.

Inventors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1377877
Patent Number(s):
9,745,610
Application Number:
14/248,980
Assignee:
The Regents of the University of California LBNL
DOE Contract Number:
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Apr 09
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Doudna, Jennifer A., Qi, Lei S., Haurwitz, Rachel E., and Arkin, Adam P. Methods and compositions for controlling gene expression by RNA processing. United States: N. p., 2017. Web.
Doudna, Jennifer A., Qi, Lei S., Haurwitz, Rachel E., & Arkin, Adam P. Methods and compositions for controlling gene expression by RNA processing. United States.
Doudna, Jennifer A., Qi, Lei S., Haurwitz, Rachel E., and Arkin, Adam P. 2017. "Methods and compositions for controlling gene expression by RNA processing". United States. doi:. https://www.osti.gov/servlets/purl/1377877.
@article{osti_1377877,
title = {Methods and compositions for controlling gene expression by RNA processing},
author = {Doudna, Jennifer A. and Qi, Lei S. and Haurwitz, Rachel E. and Arkin, Adam P.},
abstractNote = {The present disclosure provides nucleic acids encoding an RNA recognition sequence positioned proximal to an insertion site for the insertion of a sequence of interest; and host cells genetically modified with the nucleic acids. The present disclosure also provides methods of modifying the activity of a target RNA, and kits and compositions for carrying out the methods.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month = 8
}

Patent:

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  • A method of controlling expression of a DNA segment under the control of a nod gene promoter which comprises administering to a host containing a nod gene promoter an amount sufficient to control expression of the DNA segment of a compound of the formula: ##STR1## in which each R is independently H or OH, is described.
  • A method is disclosed of controlling expression of a DNA segment under the control of a nod gene promoter. This method comprises administering to a host containing a nod gene promoter an amount sufficient to control expression of the DNA segment of a compound of the formula shown in the accompanying diagram in which each R is independently H or OH, is described.
  • Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.
  • A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.
  • A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.