Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Wu, Chia-wei; Spike, Thomas; Klingeman, Dawn M.; Rodriguez, Miguel; Bremer, Virgil R.; Brown, Steven D.

doi:10.1038/s41598-017-02628-w

Title: Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Journal Article · Tue May 23 00:00:00 EDT 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-02628-w· OSTI ID:1376309

Wu, Chia-wei ^[1]; Spike, Thomas ^[2]; Klingeman, Dawn M. ^[3]; Rodriguez, Miguel ^[3]; Bremer, Virgil R. ^[4]; Brown, Steven D. ^[3]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Wisconsin, Madison, WI (United States)
Biota Biosciences, Inc., Cambridge City, IN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Elanco Animal Health, Greenfield, IN (United States)

Microorganisms are key components for plant biomass breakdown within rumen environments. Gram negative Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60-5.65, which is lower than has been reported previously. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.1 and 5.65 compared to pH 6.7, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored the function of E. coli SufDSE, suggesting a possible role for F. succinogenes sufD in acid homeostasis. Recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this genus.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1376309

Alternate ID(s):: OSTI ID: 1408644

Journal Information:: Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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