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Title: Sickle cells produce functional immune modulators and cytotoxics

 [1];  [1];  [2];  [2];  [1]; ORCiD logo [1]
  1. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, School of Medicine, Birmingham Alabama
  2. Department of Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks North Dakota
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Nuclear Physics (NP) (SC-26)
OSTI Identifier:
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
American Journal of Hematology
Additional Journal Information:
Journal Volume: 92; Journal Issue: 10; Related Information: CHORUS Timestamp: 2018-01-16 08:37:40; Journal ID: ISSN 0361-8609
Country of Publication:
United States

Citation Formats

Sun, Chiao-Wang, Wu, Li-Chen, Knopick, Peter L., Bradley, David S., Townes, Tim, and Terman, David S. Sickle cells produce functional immune modulators and cytotoxics. United States: N. p., 2017. Web. doi:10.1002/ajh.24836.
Sun, Chiao-Wang, Wu, Li-Chen, Knopick, Peter L., Bradley, David S., Townes, Tim, & Terman, David S. Sickle cells produce functional immune modulators and cytotoxics. United States. doi:10.1002/ajh.24836.
Sun, Chiao-Wang, Wu, Li-Chen, Knopick, Peter L., Bradley, David S., Townes, Tim, and Terman, David S. Sat . "Sickle cells produce functional immune modulators and cytotoxics". United States. doi:10.1002/ajh.24836.
title = {Sickle cells produce functional immune modulators and cytotoxics},
author = {Sun, Chiao-Wang and Wu, Li-Chen and Knopick, Peter L. and Bradley, David S. and Townes, Tim and Terman, David S.},
abstractNote = {},
doi = {10.1002/ajh.24836},
journal = {American Journal of Hematology},
number = 10,
volume = 92,
place = {United States},
year = {Sat Jun 24 00:00:00 EDT 2017},
month = {Sat Jun 24 00:00:00 EDT 2017}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on August 17, 2018
Publisher's Accepted Manuscript

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  • The authors have developed an animal model to elucidate the acute effects of perfusion abnormalities on muscle metabolism induced by different density-defined classes of erythrocytes isolated from sickle cell anemia patients. Technetium-99m ({sup 99m}Tc)-labeled, saline-washed normal (AA), homozygous sickle (SS), or high-density SS (SS4) erythrocytes were injected into the femoral artery of the rat and quantitative {sup 99m}Tc imaging, {sup 31}P magnetic resonance spectroscopy by surface coil at 2 teslas, and {sup 1}H magnetic resonance imaging at 0.15 tesla were performed. Between 5 and 25 {mu}l of SS4 cells was trapped in the microcirculation of the thigh. In contrast, fewermore » SS discocytes (SS2) or AA cells were trapped. After injection of SS4 cells an initial increase in inorganic phosphate was observed in the region of the thigh served by the femoral artery, intracellular pH decreased, and subsequently the proton relaxation time T{sub 1} reached a broad maximum at 18-28 hr. When T{sub 1} obtained at this time was plotted against the volume of cells trapped, an increase of T{sub 1} over the control value of 411 {plus minus} 48 msec was found that was proportional to the number of cells trapped. They conclude that the densest SS cells are most effective at producing vasoocclusion. The extent of the change detected by {sup 1}H magnetic resonance imaging is dependent on the amount of cells trapped in the microcirculation and the magnitude of the initial increase of inorganic phosphate.« less
  • Studies were performed to determine whether pre-T cells develop normally in the bone marrow of mice displaying thymic dysplasia and T cell immunodeficiency as a consequence of a graft-versus-host (GVH) reaction. GVH reactions were induced in CBAxAF1 mice by the injection of A strain lymphoid cells. To test for the presence of pre-T cells in GVH-reactive mice, bone marrow from GVH-reactive mice (GVHBM) was injected into irradiated syngeneic F1 mice and 30-40 days later thymic morphology and function were studied. Morphology studies showed nearly normal thymic architectural restoration; moreover, such glands contained normal numbers of Thy-1-positive cells. Functional pre-T cellsmore » were evaluated by transferring thymocytes from the irradiated GVHBM-reconstituted mice into T-cell-deprived mice. These thymocytes reconstituted allograft reactivity, T helper cell function and Con A and PHA mitogen responses of T-cell-deprived mice. These results suggest that the pre-T cell population in the bone marrow is not affected by the GVH reaction. Therefore, the T cell immunodeficiency associated with the GVH reaction is not due to a deficiency of pre-T cells in the bone marrow but is more likely associated with GVH-induced thymic dysplasia.« less
  • Through the use of P/sup 32/ a coefficient of transfer and a coefficient of inhibition of transfer are defined precisely for the transport of phosphate into the human red blood cell. The method is applied to a study of the inhibitory effect of sodium fluoride on the uptake of radiophosphate by sickle cell red blood cells. The data reveal that there may be an intrinsic quality in the sickle cell which is different in the normal cell since the rate of transfer of phosphate in the sickle cell is the same with or without sodium fluoride while transport in themore » normal red blood cell is strongly inhibited by fluoride. (auth)« less
  • A young patient with sickle cell disease (SCD) and multiple hospitalizations for crisis was admitted because of suspected osteomyelitis. Initial laboratory work, radiographs, and bone images were not contributory. An In-111 white blood cell (WBC) study demonstrated two areas of increased radionuclide uptake consistent with osteomyelitis. One of these had associated soft tissue infection. No other areas of active osteomyelitis were visualized, in spite of the presence of several additional infection sites. Imaging with In-111 WBC is probably not justified for routine diagnosis of acute osteomyelitis in areas free of previous disease, where conventional bone images are highly efficient. In-111more » WBC imaging, however, may be helpful in detecting osteomyelitis in selected patients with SCD in whom Tc-99m bone images and radiographs are usually abnormal and difficult to interpret due to previous bone infarcts. Localization of the infection focus is very important in choosing the aspiration site for bacteriologic studies. A negative study, however, should be interpreted cautiously.« less