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Title: Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius

Abstract

Alicyclobacillus acidocaldarius is a thermoacidophilic bacterium capable of growth on sugars from plant biomass. Carbon catabolite repression (CCR) allows bacteria to focus cellular resources on a sugar that provides efficient growth, but also allows sequential, rather than simultaneous use when more than one sugar is present. The A. acidocaldarius genome encodes all components of CCR, but transporters encoded are multifacilitator superfamily and ATP-binding cassette-type transporters, uncommon for CCR. Therefore, global transcriptome analysis of A. acidocaldarius grown on xylose or fructose was performed in this paper in chemostats, followed by attempted induction of CCR with glucose or arabinose. Alicyclobacillus acidocaldarius grew while simultaneously metabolizing xylose and glucose, xylose and arabinose, and fructose and glucose, indicating that CCR did not control carbon metabolism. Finally, microarrays showed down-regulation of genes during growth on one sugar compared to two, and occurred primarily in genes encoding: (1) regulators; (2) enzymes for cell wall synthesis; and (3) sugar transporters.

Authors:
ORCiD logo [1];  [2];  [3];  [4]
  1. Idaho National Lab. (INL), Idaho Falls, ID (United States); Idaho State Univ., Pocatello, ID (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Idaho National Lab. (INL), Idaho Falls, ID (United States); Aspenglow Associates, LLC, Jackson, WY (United States)
  3. South Dakota School of Mines and Technology, Rapid City, SD (United States)
  4. Idaho State Univ., Pocatello, ID (United States)
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE Office of Nuclear Energy (NE); INL Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1375243
Report Number(s):
INL/JOU-16-39289
Journal ID: ISSN 1367-5435; PII: 1968
Grant/Contract Number:  
AC07-05ID14517
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 44; Journal Issue: 10; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; alicyclobacillus acidocaldarius; lignocellulose; microarray; thermoacidophile

Citation Formats

Lee, Brady D., Apel, William A., DeVeaux, Linda C., and Sheridan, Peter P. Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius. United States: N. p., 2017. Web. doi:10.1007/s10295-017-1968-2.
Lee, Brady D., Apel, William A., DeVeaux, Linda C., & Sheridan, Peter P. Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius. United States. doi:10.1007/s10295-017-1968-2.
Lee, Brady D., Apel, William A., DeVeaux, Linda C., and Sheridan, Peter P. Thu . "Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius". United States. doi:10.1007/s10295-017-1968-2. https://www.osti.gov/servlets/purl/1375243.
@article{osti_1375243,
title = {Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius},
author = {Lee, Brady D. and Apel, William A. and DeVeaux, Linda C. and Sheridan, Peter P.},
abstractNote = {Alicyclobacillus acidocaldarius is a thermoacidophilic bacterium capable of growth on sugars from plant biomass. Carbon catabolite repression (CCR) allows bacteria to focus cellular resources on a sugar that provides efficient growth, but also allows sequential, rather than simultaneous use when more than one sugar is present. The A. acidocaldarius genome encodes all components of CCR, but transporters encoded are multifacilitator superfamily and ATP-binding cassette-type transporters, uncommon for CCR. Therefore, global transcriptome analysis of A. acidocaldarius grown on xylose or fructose was performed in this paper in chemostats, followed by attempted induction of CCR with glucose or arabinose. Alicyclobacillus acidocaldarius grew while simultaneously metabolizing xylose and glucose, xylose and arabinose, and fructose and glucose, indicating that CCR did not control carbon metabolism. Finally, microarrays showed down-regulation of genes during growth on one sugar compared to two, and occurred primarily in genes encoding: (1) regulators; (2) enzymes for cell wall synthesis; and (3) sugar transporters.},
doi = {10.1007/s10295-017-1968-2},
journal = {Journal of Industrial Microbiology and Biotechnology},
issn = {1367-5435},
number = 10,
volume = 44,
place = {United States},
year = {2017},
month = {8}
}

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