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Title: Reversible, on-demand generation of aqueous two-phase microdroplets

Abstract

The present invention provides methods of on-demand, reversible generation of aqueous two-phase microdroplets core-shell microbeads, microparticle preparations comprising the core-shell microbeads, and drug delivery formulation comprising the microparticle preparations. Because these aqueous microdroplets have volumes comparable to those of cells, they provide an approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Hence, the present methods generate femtoliter aqueous two-phase droplets within a microfluidic oil channel using gated pressure pulses to generate individual, stationary two-phase microdroplets with a well-defined time zero for carrying out controlled and sequential phase transformations over time. Reversible phase transitions between single-phase, two-phase, and core-shell microbead states are obtained via evaporation-induced dehydration and water rehydration.

Inventors:
; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1375237
Patent Number(s):
9,730,898
Application Number:
13/970,724
Assignee:
UT-BATTELLE, LLC ORNL
DOE Contract Number:
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Aug 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, and Mruetusatorn, Prachya. Reversible, on-demand generation of aqueous two-phase microdroplets. United States: N. p., 2017. Web.
Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, & Mruetusatorn, Prachya. Reversible, on-demand generation of aqueous two-phase microdroplets. United States.
Collier, Charles Patrick, Retterer, Scott Thomas, Boreyko, Jonathan Barton, and Mruetusatorn, Prachya. 2017. "Reversible, on-demand generation of aqueous two-phase microdroplets". United States. doi:. https://www.osti.gov/servlets/purl/1375237.
@article{osti_1375237,
title = {Reversible, on-demand generation of aqueous two-phase microdroplets},
author = {Collier, Charles Patrick and Retterer, Scott Thomas and Boreyko, Jonathan Barton and Mruetusatorn, Prachya},
abstractNote = {The present invention provides methods of on-demand, reversible generation of aqueous two-phase microdroplets core-shell microbeads, microparticle preparations comprising the core-shell microbeads, and drug delivery formulation comprising the microparticle preparations. Because these aqueous microdroplets have volumes comparable to those of cells, they provide an approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Hence, the present methods generate femtoliter aqueous two-phase droplets within a microfluidic oil channel using gated pressure pulses to generate individual, stationary two-phase microdroplets with a well-defined time zero for carrying out controlled and sequential phase transformations over time. Reversible phase transitions between single-phase, two-phase, and core-shell microbead states are obtained via evaporation-induced dehydration and water rehydration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month = 8
}

Patent:

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  • Aqueous two-phase systems contained entirely within microdroplets enable a bottom-up approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Here, we demonstrate the on-demand generation of femtolitre aqueous two-phase droplets within a microfluidic oil channel. Gated pressure pulses were used to generate individual, stationary two-phase microdroplets with a well-defined time zero for carrying out controlled and sequential phase transformations over time. Reversible phase transitions between single-phase, two-phase, and core-shell microgel states were obtained via evaporation-induced dehydration and on-demand water rehydration. In contrast to other microfluidic aqueous two-phase droplets, which require continuous flows andmore » high-frequency droplet formation, our system enables the controlled isolation and reversible transformation of a single microdroplet and is expected to be useful for future studies in dynamic microcompartmentation and affinity partitioning.« less
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  • This patent describes a reversible liquid/solid phase change composition having a melting temperature of from about 5/sup 0/ to about 50/sup 0/C. The composition comprises a hydrated mixture of from about 20 to about 67 weight percent CaBr/sub 2/, from greater than zero to about 38 weight percent CaCl/sub 2/, from about 28 to about 50 weight percent water, and a modifier selected from the group consisting of KBr, and mixtures of KBr and KCl. The modifier is present in an amount greater than zero to less than about 10 weight percent and sufficient to modify the semicongruent melting behaviormore » of the CaBr/sub 2//CaCl/sub 2/ mixture to reduce, during freezing of the composition, the formation of crystalline CaBr/sub 2/ and CaCl/sub 2/ hydrate phases other than the hexahydrate phase.« less
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