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Title: Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

Abstract

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

Inventors:
; ; ;
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1375233
Patent Number(s):
9,732,330
Application Number:
15/074,297
Assignee:
Battelle Energy Alliance, LLC INL
DOE Contract Number:  
AC07-99ID13727; AC07-05ID14517
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Mar 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Thompson, David N., Apel, William A., Thompson, Vicki S., and Ward, Thomas E.. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes. United States: N. p., 2017. Web.
Thompson, David N., Apel, William A., Thompson, Vicki S., & Ward, Thomas E.. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes. United States.
Thompson, David N., Apel, William A., Thompson, Vicki S., and Ward, Thomas E.. Tue . "Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes". United States. doi:. https://www.osti.gov/servlets/purl/1375233.
@article{osti_1375233,
title = {Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes},
author = {Thompson, David N. and Apel, William A. and Thompson, Vicki S. and Ward, Thomas E.},
abstractNote = {A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 15 00:00:00 EDT 2017},
month = {Tue Aug 15 00:00:00 EDT 2017}
}

Patent:

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Works referenced in this record:

Polypeptides of Alicyclobacillus sp.
patent-application, April 2007


A new class of biodegradable materials: Poly-3-hydroxy-butyrate/steam exploded straw fiber composites. I. Thermal and impact behavior
journal, September 1993

  • Avella, Maurizio; Martuscelli, Ezio; Pascucci, Beniamino
  • Journal of Applied Polymer Science, Vol. 49, Issue 12, p. 2091-2103
  • DOI: 10.1002/app.1993.070491205

Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000


Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Comparison of the economics of acid and enzymatic hydrolysis of newsprint
journal, April 1978

  • Grethlein, Hans E.
  • Biotechnology and Bioengineering, Vol. 20, Issue 4, p. 503-525
  • DOI: 10.1002/bit.260200404

Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis
journal, July 1980

  • Knappert, Diane; Grethlein, Hans; Converse, Alvin
  • Biotechnology and Bioengineering, Vol. 22, Issue 7, p. 1449-1463
  • DOI: 10.1002/bit.260220711

Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams
journal, October 2001

  • Lee, Brady D.; Apel, William A.; Smith, William A.
  • Environmental Progress, Vol. 20, Issue 3, p. 157-166
  • DOI: 10.1002/ep.670200311

The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Biomechanical and Biochemical Pulping of Sugarcane Bagasse with Ceriporiopsis subvermispora Fungal and Xylanase Pretreatments
journal, March 2001

  • Ramos, Juan; González, Mercedes; Ramírez, Felipe
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 3, p. 1180-1186
  • DOI: 10.1021/jf0011586

Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol
journal, January 2005

  • Saha, Badal C.; Iten, Loren B.; Cotta, Michael A.
  • Biotechnology Progress, Vol. 21, Issue 3, p. 816-822
  • DOI: 10.1021/bp049564n

Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009
journal, December 1994


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga
journal, July 1991

  • Simpson, H D; Haufler, U R; Daniel, R M
  • Biochemical Journal, Vol. 277, Issue 2, p. 413-417
  • DOI: 10.1042/bj2770413

Cellulase-free xylanases from Bacillus and other microorganisms
journal, February 2000


In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium
journal, March 1998


Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor
conference, May 1997

  • Thompson, Vicki S.; Maragos, Chris M.; Thompson, Richard B.
  • Advances in Fluorescence Sensing Technology III, Vol. 2980
  • DOI: 10.1117/12.273560

Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus
journal, January 2003

  • Thompson, Vicki S.; Schaller, Kastli D.; Apel, William A.
  • Biotechnology Progress, Vol. 19, Issue 4, p. 1292-1299
  • DOI: 10.1021/bp034040t