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Thionin-D4E1 chimeric protein protects plants against bacterial infections

Patent ·
OSTI ID:1374580
The generation of a chimeric protein containing a first domain encoding either a pro-thionon or thionin, a second domain encoding D4E1 or pro-D4E1, and a third domain encoding a peptide linker located between the first domain and second domain is described. Either the first domain or the second domain is located at the amino terminal of the chimeric protein and the other domain (second domain or first domain, respectively) is located at the carboxyl terminal. The chimeric protein has antibacterial activity. Genetically altered plants and their progeny expressing a polynucleotide encoding the chimeric protein resist diseases caused by bacteria.
Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-06NA25396
Assignee:
The United States of America, as represented by the Secretary of Agriculture
Patent Number(s):
9,725,734
Application Number:
14/691,780
OSTI ID:
1374580
Country of Publication:
United States
Language:
English

References (29)

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Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta journal May 2000
Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter journal July 2013
Broad-Spectrum Antimicrobial Activity in vitro of the Synthetic Peptide D4E1
  • Rajasekaran, Kanniah; Stromberg, Kurt D.; Cary, Jeffrey W.
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 6, p. 2799-2803 https://doi.org/10.1021/jf010154d
journal June 2001
Transgenic Potatoes Expressing a Novel Cationic Peptide are Resistant to Late Blight and Pink Rot journal April 2004
Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease journal January 2004
An engineered innate immune defense protects grapevines from Pierce disease journal February 2012
Identification of Novel Hexapeptides Bioactive against Phytopathogenic Fungi through Screening of a Synthetic Peptide Combinatorial Library journal May 2002
Antimicrobial peptides and plant disease control journal May 2007
Overexpression of an Endogenous Thionin Enhances Resistance of Arabidopsis against Fusarium oxysporum journal April 1997
Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens journal March 1993
Improvement of cyclic decapeptides against plant pathogenic bacteria using a combinatorial chemistry approach journal November 2006
Inhibition of bacterial and fungal plant pathogens by thionins of types I and II journal January 1993
Bacterial disease resistance of transgenic hybrid poplar expressing the synthetic antimicrobial peptide D4E1 journal April 2003
Identification and Characterization of a Hexapeptide with Activity Against Phytopathogenic Fungi That Cause Postharvest Decay in Fruits journal August 2000
Expression of an Engineered Cecropin Gene Cassette in Transgenic Tobacco Plants Confers Disease Resistance to Pseudomonas syringae pv. tabaci journal May 1997
Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants journal November 2005
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Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots journal January 2012
Involvement of the Pepper Antimicrobial Protein CaAMP1 Gene in Broad Spectrum Disease Resistance journal August 2008
Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum journal January 1993
First evidence for improved resistance to fire blight in transgenic pear expressing the attacin E gene from Hyalophora cecropia journal November 1999
Cecropin A—Derived Peptides Are Potent Inhibitors of Fungal Plant Pathogens journal March 1998
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Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice journal October 2005
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Enhanced resistance to bacterial and fungal pathogens by overexpression of a human cathelicidin antimicrobial peptide (hCAP18/LL-37) in Chinese cabbage journal October 2011

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