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Title: Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development

Abstract

Galactinol synthase is a pivotal enzyme involved in the synthesis of the raffinose family of oligosaccharides (RFOs) that function as transport carbohydrates in the phloem, as storage compounds in sink tissues and as soluble metabolites that combat both abiotic and biotic stress in several plant species. For hybrid poplar (Populus alba 9 grandidentata) overexpressing the Arabidopsis thaliana GolS3 (AtGolS3) gene showed clear effects on development; the extreme overexpressing lines were stunted and had cell wall traits characteristic of tension wood, whereas lines with only moderate up-regulation grew normally and had moderately altered secondary cell wall composition and ultrastructure. Stem cross-sections of the developing xylem revealed a significant increase in the number of vessels, as well as the clear presence of a G-layer in the fibres. Furthermore, AtGolS3-OE lines possessed higher cellulose and lower lignin contents, an increase in cellulose crystallinity, and significantly altered hemicellulose-derived carbohydrates, notably manifested by their mannose and xylose contents. Additionally, the transgenic plants displayed elevated xylem starch content. Transcriptome interrogation of the transgenic plants showed a significant up-regulation of genes involved in the synthesis of myo-inositol, along with genes involved in sucrose degradation. Our results suggest that the over expression of GolS and its product galactinolmore » may serve as a molecular signal that initiates metabolic changes, culminating in a change in cell wall development and potentially the formation of tension wood.« less

Authors:
 [1];  [2];  [3];  [2];  [4]
  1. Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Wood Science
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Biochemistry; Wisconsin Energy Inst., Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center
  3. Agricultural Research Council, Pretoria (South Africa). Biotechnology Platform
  4. Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Wood Science; Wisconsin Energy Inst., Madison, WI (United States). Dept. of Energy Great Lakes Bioenergy Research Center
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1374538
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 15; Journal Issue: 7; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; galactinol synthase; RFOs; tension wood; cellulose; lignin; sugar signalling

Citation Formats

Unda, Faride, Kim, Hoon, Hefer, Charles, Ralph, John, and Mansfield, Shawn D. Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development. United States: N. p., 2017. Web. doi:10.1111/pbi.12682.
Unda, Faride, Kim, Hoon, Hefer, Charles, Ralph, John, & Mansfield, Shawn D. Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development. United States. doi:10.1111/pbi.12682.
Unda, Faride, Kim, Hoon, Hefer, Charles, Ralph, John, and Mansfield, Shawn D. Sat . "Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development". United States. doi:10.1111/pbi.12682. https://www.osti.gov/servlets/purl/1374538.
@article{osti_1374538,
title = {Altering carbon allocation in hybrid poplar ( Populus alba × grandidentata ) impacts cell wall growth and development},
author = {Unda, Faride and Kim, Hoon and Hefer, Charles and Ralph, John and Mansfield, Shawn D.},
abstractNote = {Galactinol synthase is a pivotal enzyme involved in the synthesis of the raffinose family of oligosaccharides (RFOs) that function as transport carbohydrates in the phloem, as storage compounds in sink tissues and as soluble metabolites that combat both abiotic and biotic stress in several plant species. For hybrid poplar (Populus alba 9 grandidentata) overexpressing the Arabidopsis thaliana GolS3 (AtGolS3) gene showed clear effects on development; the extreme overexpressing lines were stunted and had cell wall traits characteristic of tension wood, whereas lines with only moderate up-regulation grew normally and had moderately altered secondary cell wall composition and ultrastructure. Stem cross-sections of the developing xylem revealed a significant increase in the number of vessels, as well as the clear presence of a G-layer in the fibres. Furthermore, AtGolS3-OE lines possessed higher cellulose and lower lignin contents, an increase in cellulose crystallinity, and significantly altered hemicellulose-derived carbohydrates, notably manifested by their mannose and xylose contents. Additionally, the transgenic plants displayed elevated xylem starch content. Transcriptome interrogation of the transgenic plants showed a significant up-regulation of genes involved in the synthesis of myo-inositol, along with genes involved in sucrose degradation. Our results suggest that the over expression of GolS and its product galactinol may serve as a molecular signal that initiates metabolic changes, culminating in a change in cell wall development and potentially the formation of tension wood.},
doi = {10.1111/pbi.12682},
journal = {Plant Biotechnology Journal},
number = 7,
volume = 15,
place = {United States},
year = {Sat Mar 04 00:00:00 EST 2017},
month = {Sat Mar 04 00:00:00 EST 2017}
}

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Works referenced in this record:

Modifications in Lignin and Accumulation of Phenolic Glucosides in Poplar Xylem upon Down-regulation of Caffeoyl-Coenzyme A O-Methyltransferase, an Enzyme Involved in Lignin Biosynthesis
journal, August 2000

  • Meyermans, Hugo; Morreel, Kris; Lapierre, Catherine
  • Journal of Biological Chemistry, Vol. 275, Issue 47, p. 36899-36909
  • DOI: 10.1074/jbc.M006915200