Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice
Abstract
Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.
- Authors:
-
- Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio), Purdue University, West Lafayette IN USA; Department of Horticulture, Purdue University, West Lafayette IN USA; Department of Biological Sciences, Purdue University, West Lafayette IN USA
- Biosciences Center, National Renewable Energy Laboratory, Golden CO USA
- Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio), Purdue University, West Lafayette IN USA; Department of Horticulture, Purdue University, West Lafayette IN USA
- Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio), Purdue University, West Lafayette IN USA; Department of Biological Sciences, Purdue University, West Lafayette IN USA
- X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne IL USA
- Department of Horticulture, Purdue University, West Lafayette IN USA
- National Bioenergy Center, National Renewable Energy Laboratory, Golden CO USA
- Department of Biological Sciences, Purdue University, West Lafayette IN USA; Department of Botany and Plant Pathology, Purdue University, West Lafayette IN USA
- Department of Bioengineering, Northeastern University, Boston MA USA; Department of Chemistry and Chemical Biology, Northeastern University, Boston MA USA
- Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio), Purdue University, West Lafayette IN USA; Department of Horticulture, Purdue University, West Lafayette IN USA; Department of Plant Science and Landscape Architecture, University of Maryland, College Park MD USA
- Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio), Purdue University, West Lafayette IN USA; Department of Horticulture, Purdue University, West Lafayette IN USA; Department of Plant Science and Landscape Architecture, University of Maryland, College Park MD USA; Department of Environmental Science and Technology, University of Maryland, College Park MD USA
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1373944
- DOE Contract Number:
- AC02-06CH11357
- Resource Type:
- Journal Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 10; Journal ID: ISSN 1467-7644
- Publisher:
- Society for Experimental Biology; Association of Applied Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Biofuel; carbohydrate-binding module; cell wall; iron concentration; iron-binding peptide; secretion
Citation Formats
Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., and Peer, Wendy A. Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice. United States: N. p., 2016.
Web. doi:10.1111/pbi.12557.
Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., & Peer, Wendy A. Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice. United States. https://doi.org/10.1111/pbi.12557
Yang, Haibing, Wei, Hui, Ma, Guojie, Antunes, Mauricio S., Vogt, Stefan, Cox, Joseph, Zhang, Xiao, Liu, Xiping, Bu, Lintao, Gleber, S. Charlotte, Carpita, Nicholas C., Makowski, Lee, Himmel, Michael E., Tucker, Melvin P., McCann, Maureen C., Murphy, Angus S., and Peer, Wendy A. 2016.
"Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice". United States. https://doi.org/10.1111/pbi.12557.
@article{osti_1373944,
title = {Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice},
author = {Yang, Haibing and Wei, Hui and Ma, Guojie and Antunes, Mauricio S. and Vogt, Stefan and Cox, Joseph and Zhang, Xiao and Liu, Xiping and Bu, Lintao and Gleber, S. Charlotte and Carpita, Nicholas C. and Makowski, Lee and Himmel, Michael E. and Tucker, Melvin P. and McCann, Maureen C. and Murphy, Angus S. and Peer, Wendy A.},
abstractNote = {Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.},
doi = {10.1111/pbi.12557},
url = {https://www.osti.gov/biblio/1373944},
journal = {Plant Biotechnology Journal},
issn = {1467-7644},
number = 10,
volume = 14,
place = {United States},
year = {Thu Apr 07 00:00:00 EDT 2016},
month = {Thu Apr 07 00:00:00 EDT 2016}
}
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