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Title: Chemically Stable Lipids for Membrane Protein Crystallization

Abstract

The lipidic cubic phase (LCP) has been widely recognized as a promising membrane-mimicking matrix for biophysical studies of membrane proteins and their crystallization in a lipidic environment. Application of this material to a wide variety of membrane proteins, however, is hindered due to a limited number of available host lipids, mostly monoacylglycerols (MAGs). Here, we designed, synthesized, and characterized a series of chemically stable lipids resistant to hydrolysis, with properties complementary to the widely used MAGs. In order to assess their potential to serve as host lipids for crystallization, we characterized the phase properties and lattice parameters of mesophases made of two most promising lipids at a variety of different conditions by polarized light microscopy and small-angle X-ray scattering. Both lipids showed remarkable chemical stability and an extended LCP region in the phase diagram covering a wide range of temperatures down to 4 °C. One of these lipids has been used for crystallization and structure determination of a prototypical membrane protein bacteriorhodopsin at 4 and 20 °C.

Authors:
ORCiD logo; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1373782
Resource Type:
Journal Article
Resource Relation:
Journal Name: Crystal Growth and Design; Journal Volume: 17; Journal Issue: 6
Country of Publication:
United States
Language:
ENGLISH
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Ishchenko, Andrii, Peng, Lingling, Zinovev, Egor, Vlasov, Alexey, Lee, Sung Chang, Kuklin, Alexander, Mishin, Alexey, Borshchevskiy, Valentin, Zhang, Qinghai, and Cherezov, Vadim. Chemically Stable Lipids for Membrane Protein Crystallization. United States: N. p., 2017. Web. doi:10.1021/acs.cgd.7b00458.
Ishchenko, Andrii, Peng, Lingling, Zinovev, Egor, Vlasov, Alexey, Lee, Sung Chang, Kuklin, Alexander, Mishin, Alexey, Borshchevskiy, Valentin, Zhang, Qinghai, & Cherezov, Vadim. Chemically Stable Lipids for Membrane Protein Crystallization. United States. doi:10.1021/acs.cgd.7b00458.
Ishchenko, Andrii, Peng, Lingling, Zinovev, Egor, Vlasov, Alexey, Lee, Sung Chang, Kuklin, Alexander, Mishin, Alexey, Borshchevskiy, Valentin, Zhang, Qinghai, and Cherezov, Vadim. Mon . "Chemically Stable Lipids for Membrane Protein Crystallization". United States. doi:10.1021/acs.cgd.7b00458.
@article{osti_1373782,
title = {Chemically Stable Lipids for Membrane Protein Crystallization},
author = {Ishchenko, Andrii and Peng, Lingling and Zinovev, Egor and Vlasov, Alexey and Lee, Sung Chang and Kuklin, Alexander and Mishin, Alexey and Borshchevskiy, Valentin and Zhang, Qinghai and Cherezov, Vadim},
abstractNote = {The lipidic cubic phase (LCP) has been widely recognized as a promising membrane-mimicking matrix for biophysical studies of membrane proteins and their crystallization in a lipidic environment. Application of this material to a wide variety of membrane proteins, however, is hindered due to a limited number of available host lipids, mostly monoacylglycerols (MAGs). Here, we designed, synthesized, and characterized a series of chemically stable lipids resistant to hydrolysis, with properties complementary to the widely used MAGs. In order to assess their potential to serve as host lipids for crystallization, we characterized the phase properties and lattice parameters of mesophases made of two most promising lipids at a variety of different conditions by polarized light microscopy and small-angle X-ray scattering. Both lipids showed remarkable chemical stability and an extended LCP region in the phase diagram covering a wide range of temperatures down to 4 °C. One of these lipids has been used for crystallization and structure determination of a prototypical membrane protein bacteriorhodopsin at 4 and 20 °C.},
doi = {10.1021/acs.cgd.7b00458},
journal = {Crystal Growth and Design},
number = 6,
volume = 17,
place = {United States},
year = {Mon May 01 00:00:00 EDT 2017},
month = {Mon May 01 00:00:00 EDT 2017}
}