skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate

Abstract

Here, interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, and is expected to increase biofuel yield variability. However, the effect of precipitation on downstream fermentation processes has never been directly characterized. In order to investigate the impact of interannual climate variability on biofuel production, corn stover and switchgrass were collected during 3 years with significantly different precipitation profiles, representing a major drought year (2012) and 2 years with average precipitation for the entire season (2010 and 2013). All feedstocks were AFEX (ammonia fiber expansion)-pretreated, enzymatically hydrolyzed, and the hydrolysates separately fermented using xylose-utilizing strains of Saccharomyces cerevisiae and Zymomonas mobilis. As a result, a chemical genomics approach was also used to evaluate the growth of yeast mutants in the hydrolysates.

Authors:
 [1];  [2];  [2];  [2];  [2];  [3];  [2];  [2];  [3];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [3];  [2]
  1. Michigan State Univ., East Lansing, MI (United States); Michigan Technological Univ., Houghton, MI (United States)
  2. Univ. of Wisconsin, Madison, WI (United States)
  3. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1373396
Grant/Contract Number:  
FC02-07ER64494; AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Ong, Rebecca Garlock, Higbee, Alan, Bottoms, Scott, Dickinson, Quinn, Xie, Dan, Smith, Scott A., Serate, Jose, Pohlmann, Edward, Jones, Arthur Daniel, Coon, Joshua J., Sato, Trey K., Sanford, Gregg R., Eilert, Dustin, Oates, Lawrence G., Piotrowski, Jeff S., Bates, Donna M., Cavalier, David, and Zhang, Yaoping. Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate. United States: N. p., 2016. Web. doi:10.1186/s13068-016-0657-0.
Ong, Rebecca Garlock, Higbee, Alan, Bottoms, Scott, Dickinson, Quinn, Xie, Dan, Smith, Scott A., Serate, Jose, Pohlmann, Edward, Jones, Arthur Daniel, Coon, Joshua J., Sato, Trey K., Sanford, Gregg R., Eilert, Dustin, Oates, Lawrence G., Piotrowski, Jeff S., Bates, Donna M., Cavalier, David, & Zhang, Yaoping. Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate. United States. doi:10.1186/s13068-016-0657-0.
Ong, Rebecca Garlock, Higbee, Alan, Bottoms, Scott, Dickinson, Quinn, Xie, Dan, Smith, Scott A., Serate, Jose, Pohlmann, Edward, Jones, Arthur Daniel, Coon, Joshua J., Sato, Trey K., Sanford, Gregg R., Eilert, Dustin, Oates, Lawrence G., Piotrowski, Jeff S., Bates, Donna M., Cavalier, David, and Zhang, Yaoping. Tue . "Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate". United States. doi:10.1186/s13068-016-0657-0. https://www.osti.gov/servlets/purl/1373396.
@article{osti_1373396,
title = {Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate},
author = {Ong, Rebecca Garlock and Higbee, Alan and Bottoms, Scott and Dickinson, Quinn and Xie, Dan and Smith, Scott A. and Serate, Jose and Pohlmann, Edward and Jones, Arthur Daniel and Coon, Joshua J. and Sato, Trey K. and Sanford, Gregg R. and Eilert, Dustin and Oates, Lawrence G. and Piotrowski, Jeff S. and Bates, Donna M. and Cavalier, David and Zhang, Yaoping},
abstractNote = {Here, interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, and is expected to increase biofuel yield variability. However, the effect of precipitation on downstream fermentation processes has never been directly characterized. In order to investigate the impact of interannual climate variability on biofuel production, corn stover and switchgrass were collected during 3 years with significantly different precipitation profiles, representing a major drought year (2012) and 2 years with average precipitation for the entire season (2010 and 2013). All feedstocks were AFEX (ammonia fiber expansion)-pretreated, enzymatically hydrolyzed, and the hydrolysates separately fermented using xylose-utilizing strains of Saccharomyces cerevisiae and Zymomonas mobilis. As a result, a chemical genomics approach was also used to evaluate the growth of yeast mutants in the hydrolysates.},
doi = {10.1186/s13068-016-0657-0},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {Tue Nov 08 00:00:00 EST 2016},
month = {Tue Nov 08 00:00:00 EST 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 5 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
journal, September 2005

  • Subramanian, A.; Tamayo, P.; Mootha, V. K.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 43, p. 15545-15550
  • DOI: 10.1073/pnas.0506580102

Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)
journal, January 2009

  • Lau, M. W.; Dale, B. E.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 5, p. 1368-1373
  • DOI: 10.1073/pnas.0812364106