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Title: Regulatory sequence of cupin family gene

Abstract

This invention is in the field of plant biology and agriculture and relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions.

Inventors:
;
Publication Date:
Research Org.:
ARKANSAS STATE UNIVERSITY, State University, AR (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1372666
Patent Number(s):
9,714,429
Application Number:
14/606,552
Assignee:
ARKANSAS STATE UNIVERSITY GFO
DOE Contract Number:
FG36-08GO88025
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Jan 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hood, Elizabeth, and Teoh, Thomas. Regulatory sequence of cupin family gene. United States: N. p., 2017. Web.
Hood, Elizabeth, & Teoh, Thomas. Regulatory sequence of cupin family gene. United States.
Hood, Elizabeth, and Teoh, Thomas. 2017. "Regulatory sequence of cupin family gene". United States. doi:. https://www.osti.gov/servlets/purl/1372666.
@article{osti_1372666,
title = {Regulatory sequence of cupin family gene},
author = {Hood, Elizabeth and Teoh, Thomas},
abstractNote = {This invention is in the field of plant biology and agriculture and relates to novel seed specific promoter regions. The present invention further provide methods of producing proteins and other products of interest and methods of controlling expression of nucleic acid sequences of interest using the seed specific promoter regions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month = 7
}

Patent:

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  • The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
  • The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
  • NAD(P)H:quinone oxidoreductases (NQOs) are flavoproteins that catalyze the oxidation of NADH or NADPH by various quinones and oxidation-reduction dyes. The authors previously described a complementary DNA that encodes a dioxin-inducible cytosolic form of human NAD(P)H:quinone oxidoreductase (NQO{sub 1}). In the present report they describe the nucleotide sequence and deduced amino acid sequence for a cDNA clone that is likely to encode a second form of NAD(P)H:quinone oxidoreductase (NQO{sub 2}) which was isolated by screening a human liver cDNA library by hybridization with a NQO{sub 1} cDNA probe. The NQO{sub 2} cDNA is 976 nucleotides long and encodes a protein ofmore » 231 amino acids. COS1 cells transfected with NQO{sub 2} cDNA showed a 5-7 fold increase in NAD(P)H:quinone oxidoreductase activity as compared to nontransfected cells when either 2,6-dichlorophenolindophenol or menadione was used as substrate. The NQO{sub 2} gene locus is highly polymorphic as indicated by several restriction fragment length polymorphisms detected with five different restriction enzymes. The NQO{sub 2} gene was localized to human chromosome 6 by Southern analysis of human-rodent somatic cell hybrids.« less
  • Research highlights: {yields} SV40-DTS worked as a DTS in ES cells as well as other types of cells. {yields} Sox2 regulatory region 2 worked as a DTS in ES cells and thus was termed as SRR2-DTS. {yields} SRR2-DTS was suggested as an ES cell-specific DTS. -- Abstract: In this report, the effects of two DNA nuclear targeting sequence (DTS) candidates on the gene expression efficiency in ES cells were investigated. Reporter plasmids containing the simian virus 40 (SV40) promoter/enhancer sequence (SV40-DTS), a DTS for various types of cells but not being reported yet for ES cells, and the 81 basemore » pairs of Sox2 regulatory region 2 (SRR2) where two transcriptional factors in ES cells, Oct3/4 and Sox2, are bound (SRR2-DTS), were introduced into cytoplasm in living cells by femtoinjection. The gene expression efficiencies of each plasmid in mouse insulinoma cell line MIN6 cells and mouse ES cells were then evaluated. Plasmids including SV40-DTS and SRR2-DTS exhibited higher gene expression efficiency comparing to plasmids without these DTSs, and thus it was concluded that both sequences work as a DTS in ES cells. In addition, it was suggested that SRR2-DTS works as an ES cell-specific DTS. To the best of our knowledge, this is the first report to confirm the function of DTSs in ES cells.« less
  • Nucleic acid sequences and methods for their use are described which provide for early seed-specific transcription, in order to modulate or modify expression of foreign or endogenous genes in seeds, particularly embryo cells. The method finds particular use in conjunction with modifying fatty acid production in seed tissue.