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Title: Contact Mechanics of a Small Icosahedral Virus

Authors:
; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1372135
Grant/Contract Number:
SC0010507
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Physical Review Letters
Additional Journal Information:
Journal Volume: 119; Journal Issue: 3; Related Information: CHORUS Timestamp: 2017-07-20 22:13:37; Journal ID: ISSN 0031-9007
Publisher:
American Physical Society
Country of Publication:
United States
Language:
English

Citation Formats

Zeng, Cheng, Hernando-Pérez, Mercedes, Dragnea, Bogdan, Ma, Xiang, van der Schoot, Paul, and Zandi, Roya. Contact Mechanics of a Small Icosahedral Virus. United States: N. p., 2017. Web. doi:10.1103/PhysRevLett.119.038102.
Zeng, Cheng, Hernando-Pérez, Mercedes, Dragnea, Bogdan, Ma, Xiang, van der Schoot, Paul, & Zandi, Roya. Contact Mechanics of a Small Icosahedral Virus. United States. doi:10.1103/PhysRevLett.119.038102.
Zeng, Cheng, Hernando-Pérez, Mercedes, Dragnea, Bogdan, Ma, Xiang, van der Schoot, Paul, and Zandi, Roya. 2017. "Contact Mechanics of a Small Icosahedral Virus". United States. doi:10.1103/PhysRevLett.119.038102.
@article{osti_1372135,
title = {Contact Mechanics of a Small Icosahedral Virus},
author = {Zeng, Cheng and Hernando-Pérez, Mercedes and Dragnea, Bogdan and Ma, Xiang and van der Schoot, Paul and Zandi, Roya},
abstractNote = {},
doi = {10.1103/PhysRevLett.119.038102},
journal = {Physical Review Letters},
number = 3,
volume = 119,
place = {United States},
year = 2017,
month = 7
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on July 20, 2018
Publisher's Accepted Manuscript

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  • Sulfolobus turreted icosahedral virus (STIV) was the first non-tailed icosahedral virus to be isolated from an archaeal host. Like other archaeal viruses, its 37 open reading frames generally lack sequence similarity to genes with known function. The roles of the gene products in this and other archaeal viruses are thus largely unknown. However, a protein's three-dimensional structure may provide functional and evolutionary insight in cases of minimal sequence similarity. In this vein, the structure of STIV F93 reveals a homodimer with strong similarity to the winged-helix family of DNA-binding proteins. Importantly, an interchain disulfide bond is found at the dimermore » interface, prompting analysis of the cysteine distribution in the putative intracellular proteins of the viral proteome. The analysis suggests that intracellular disulfide bonds are common in cellular STIV proteins, where they enhance the thermostability of the viral proteome.« less
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  • No abstract prepared.
  • The constraints imposed on structure-factor phases by noncrystallographic symmetry (NCS) allow phase improvement, phase extension to higher resolution and hence ab initio phase determination. The more numerous the NCS redundancy and the greater the volume used for solvent flattening, the greater the power for phase determination. In a case analyzed here the icosahedral NCS phasing appeared to have broken down, although later successful phase extension was possible when the envelope around the NCS region was tightened. The phases from the failed phase-determination attempt fell into four classes, all of which satisfied the NCS constraints. These four classes corresponded to themore » correct solution, opposite enantiomorph, Babinet inversion and opposite enantiomorph with Babinet inversion. These incorrect solutions can be seeded from structure factors belonging to reciprocal-space volumes that lie close to icosahedral NCS axes where the structure amplitudes tend to be large and the phases tend to be 0 or {pi}. Furthermore, the false solutions can spread more easily if there are large errors in defining the envelope designating the region in which NCS averaging is performed.« less