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Title: Bacterial Biogeography across the Amazon River-Ocean Continuum

Abstract

Spatial and temporal patterns in microbial biodiversity across the Amazon river-ocean continuum were investigated along ~675 km of the lower Amazon River mainstem, in the Tapajos River tributary, and in the plume and coastal ocean during low and high river discharge using amplicon sequencing of 16S rRNA genes in whole water and size-fractionated samples (0.2-2.0 μm and >2.0 μm). River communities varied among tributaries, but mainstem communities were spatially homogeneous and tracked seasonal changes in river discharge and co-varying factors. Co-occurrence network analysis identified strongly interconnected river assemblages during high (May) and low (December) discharge periods, and weakly interconnected transitional assemblages in September, suggesting that this system supports two seasonal microbial communities linked to river discharge. In contrast, plume communities showed little seasonal differences and instead varied spatially tracking salinity. However, salinity explained only a small fraction of community variability, and plume communities in blooms of diatom-diazotroph assemblages were strikingly different than those in other high salinity plume samples. This suggests that while salinity physically structures plumes through buoyancy and mixing, the composition of plume-specific communities is controlled by other factors including nutrients, phytoplankton community composition, and dissolved organic matter chemistry. Co-occurrence networks identified interconnected assemblages associated with the highlymore » productive low salinity nearshore region, diatom-diazotroph blooms, and the plume edge region, and weakly interconnected assemblages in high salinity regions. This suggests that the plume supports a transitional community influenced by immigration of ocean bacteria from the plume edge, and by species sorting as these communities adapt to local environmental conditions. Few studies have explored patterns of microbial diversity in tropical rivers and coastal oceans. Comparison of Amazon continuum microbial communities to those from temperate and arctic systems suggest that river discharge and salinity are master variables structuring a range of environmental conditions that control bacterial communities across the river-ocean continuum.« less

Authors:
 [1];  [2];  [2];  [1];  [3];  [4];  [2];  [5];  [6];  [7];  [4];  [8];  [5]
  1. University of Maryland Center for Environmental Science, Cambridge, MD (United States). Horn Point Laboratory
  2. Univ. of Georgia, Athens, GA (United States). Department of Marine Sciences
  3. Marine Biological Laboratory, Woods Hole, MA (United States). Josephine Bay Paul Center
  4. University of São Paulo, Piracicaba (Brazil). Center of Nuclear Energy in Agriculture
  5. Oregon State Univ., Corvallis, OR (United States). College of Earth, Ocean, and Atmospheric Sciences
  6. Univ. of Washington, Seattle, WA (United States). School of Oceanography
  7. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  8. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Marine Sciences Laboratory
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1371969
Report Number(s):
PNNL-SA-125962
Journal ID: ISSN 1664-302X
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Amazon; aquatic; marine; continuum; terrestrial; interface; ecosystem; microbial; bacteria; genomic; metagenome; river; coastal; estuarine; carbon; sequence; biology

Citation Formats

Doherty, Mary, Yager, Patricia L., Moran, Mary Ann, Coles, Victoria J., Fortunato, Caroline S., Krusche, Alex V., Medeiros, Patricia M., Payet, Jerome P., Richey, Jeffrey E., Satinsky, Brandon M., Sawakuchi, Henrique O., Ward, Nicholas D., and Crump, Byron C. Bacterial Biogeography across the Amazon River-Ocean Continuum. United States: N. p., 2017. Web. doi:10.3389/fmicb.2017.00882.
Doherty, Mary, Yager, Patricia L., Moran, Mary Ann, Coles, Victoria J., Fortunato, Caroline S., Krusche, Alex V., Medeiros, Patricia M., Payet, Jerome P., Richey, Jeffrey E., Satinsky, Brandon M., Sawakuchi, Henrique O., Ward, Nicholas D., & Crump, Byron C. Bacterial Biogeography across the Amazon River-Ocean Continuum. United States. doi:10.3389/fmicb.2017.00882.
Doherty, Mary, Yager, Patricia L., Moran, Mary Ann, Coles, Victoria J., Fortunato, Caroline S., Krusche, Alex V., Medeiros, Patricia M., Payet, Jerome P., Richey, Jeffrey E., Satinsky, Brandon M., Sawakuchi, Henrique O., Ward, Nicholas D., and Crump, Byron C. Tue . "Bacterial Biogeography across the Amazon River-Ocean Continuum". United States. doi:10.3389/fmicb.2017.00882. https://www.osti.gov/servlets/purl/1371969.
@article{osti_1371969,
title = {Bacterial Biogeography across the Amazon River-Ocean Continuum},
author = {Doherty, Mary and Yager, Patricia L. and Moran, Mary Ann and Coles, Victoria J. and Fortunato, Caroline S. and Krusche, Alex V. and Medeiros, Patricia M. and Payet, Jerome P. and Richey, Jeffrey E. and Satinsky, Brandon M. and Sawakuchi, Henrique O. and Ward, Nicholas D. and Crump, Byron C.},
abstractNote = {Spatial and temporal patterns in microbial biodiversity across the Amazon river-ocean continuum were investigated along ~675 km of the lower Amazon River mainstem, in the Tapajos River tributary, and in the plume and coastal ocean during low and high river discharge using amplicon sequencing of 16S rRNA genes in whole water and size-fractionated samples (0.2-2.0 μm and >2.0 μm). River communities varied among tributaries, but mainstem communities were spatially homogeneous and tracked seasonal changes in river discharge and co-varying factors. Co-occurrence network analysis identified strongly interconnected river assemblages during high (May) and low (December) discharge periods, and weakly interconnected transitional assemblages in September, suggesting that this system supports two seasonal microbial communities linked to river discharge. In contrast, plume communities showed little seasonal differences and instead varied spatially tracking salinity. However, salinity explained only a small fraction of community variability, and plume communities in blooms of diatom-diazotroph assemblages were strikingly different than those in other high salinity plume samples. This suggests that while salinity physically structures plumes through buoyancy and mixing, the composition of plume-specific communities is controlled by other factors including nutrients, phytoplankton community composition, and dissolved organic matter chemistry. Co-occurrence networks identified interconnected assemblages associated with the highly productive low salinity nearshore region, diatom-diazotroph blooms, and the plume edge region, and weakly interconnected assemblages in high salinity regions. This suggests that the plume supports a transitional community influenced by immigration of ocean bacteria from the plume edge, and by species sorting as these communities adapt to local environmental conditions. Few studies have explored patterns of microbial diversity in tropical rivers and coastal oceans. Comparison of Amazon continuum microbial communities to those from temperate and arctic systems suggest that river discharge and salinity are master variables structuring a range of environmental conditions that control bacterial communities across the river-ocean continuum.},
doi = {10.3389/fmicb.2017.00882},
journal = {Frontiers in Microbiology},
number = ,
volume = 8,
place = {United States},
year = {Tue May 23 00:00:00 EDT 2017},
month = {Tue May 23 00:00:00 EDT 2017}
}

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