Transport capabilities of environmental Pseudomonads for sulfur compounds

Zerbs, Sarah; Korajczyk, Peter J.; Noirot, Philippe H.; Collart, Frank R.

doi:10.1002/pro.3124

Title: Transport capabilities of environmental Pseudomonads for sulfur compounds

Journal Article · Fri Jan 27 00:00:00 EST 2017 · Protein Science

DOI:https://doi.org/10.1002/pro.3124· OSTI ID:1371913

Zerbs, Sarah ^[1]; Korajczyk, Peter J. ^[1]; Noirot, Philippe H. ^[1]; Collart, Frank R. ^[1]

Argonne National Lab. (ANL), Lemont, IL (United States)

Abstract Sulfur is an essential element in plant rhizospheres and microbial activity plays a key role in increasing the biological availability of sulfur in soil environments. To better understand the mechanisms facilitating the exchange of sulfur‐containing molecules in soil, we profiled the binding specificities of eight previously uncharacterized ABC transporter solute‐binding proteins from plant‐associated Pseudomonads. A high‐throughput screening procedure indicated eighteen significant organosulfur binding ligands, with at least one high‐quality screening hit for each protein target. Calorimetric and spectroscopic methods were used to validate the best ligand assignments and catalog the thermodynamic properties of the protein‐ligand interactions. Two novel high‐affinity ligand‐binding activities were identified and quantified in this set of solute‐binding proteins. Bacteria were cultured in minimal media with screening library components supplied as the sole sulfur sources, demonstrating that these organosulfur compounds can be metabolized and confirming the relevance of ligand assignments. These results expand the set of experimentally validated ligands amenable to transport by this ABC transporter family and demonstrate the complex range of protein‐ligand interactions that can be accomplished by solute‐binding proteins. Characterizing new nutrient import pathways provides insight into Pseudomonad metabolic capabilities which can be used to further interrogate bacterial survival and participation in soil and rhizosphere communities.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1371913

Alternate ID(s):: OSTI ID: 1401894

Journal Information:: Protein Science, Vol. 26, Issue 4; ISSN 0961-8368

Publisher:: The Protein SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 4 works

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