Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy- l -fucose induces defects in root cell elongation
Abstract
Screening of commercially available fluoro monosaccharides as putative growth inhibitors in Arabidopsis thaliana revealed that 2-fluoro 2-l-fucose (2F-Fuc) reduces root growth at micromolar concentrations. The inability of 2F-Fuc to affect an Atfkgp mutant that is defective in the fucose salvage pathway indicates that 2F-Fuc must be converted to its cognate GDP nucleotide sugar in order to inhibit root growth. Chemical analysis of cell wall polysaccharides and glycoproteins demonstrated that fucosylation of xyloglucans and of N-linked glycans is fully inhibited by 10 μm 2F-Fuc in Arabidopsis seedling roots, but genetic evidence indicates that these alterations are not responsible for the inhibition of root development by 2F-Fuc. Inhibition of fucosylation of cell wall polysaccharides also affected pectic rhamnogalacturonan-II (RG-II). At low concentrations, 2F-Fuc induced a decrease in RG-II dimerization. Both RG-II dimerization and root growth were partially restored in 2F-Fuc-treated seedlings by addition of boric acid, suggesting that the growth phenotype caused by 2F-Fuc was due to a deficiency of RG-II dimerization. Closer investigation of the 2F-Fuc-induced growth phenotype demonstrated that cell division is not affected by 2F-Fuc treatments. In contrast, the inhibitor suppressed elongation of root cells and promoted the emergence of adventitious roots. This study further emphasizes the importance ofmore »
- Authors:
-
- Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, EA 4358, IRIB, VASI, Normandie Université, 76821 Mont-Saint-Aignan France
- Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO) UMR CNRS 8182, Université de Paris Sud, 91405 Orsay France; Institut de Chimie des Substances Naturelles (ICSN) UPR CNRS 2301, 91198 Gif-sur-Yvette France; Click4Tag, Zone Luminy Biotech, Case 922, 163 Avenue de Luminy 13009 Marseille France
- Centre de Recherches sur les Macromolécules Végétales (CERMAV) - CNRS BP 53, 38041 Grenoble Cedex 9 France
- Department of Biology and Center for Lignocellulose Structure and Formation, Pennsylvania State University, University Park Pennsylvania USA
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1370829
- DOE Contract Number:
- SC0001090
- Resource Type:
- Journal Article
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 84; Journal Issue: 6; Related Information: CLSF partners with Pennsylvania State University (lead); North Carolina State University; University of Rhode Island; Virginia Tech University; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; biofuels (including algae and biomass), bio-inspired, membrane, carbon sequestration, materials and chemistry by design, synthesis (self-assembly)
Citation Formats
Dumont, Marie, Lehner, Arnaud, Bardor, Muriel, Burel, Carole, Vauzeilles, Boris, Lerouxel, Olivier, Anderson, Charles T., Mollet, Jean-Claude, and Lerouge, Patrice. Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy- l -fucose induces defects in root cell elongation. United States: N. p., 2015.
Web. doi:10.1111/tpj.13071.
Dumont, Marie, Lehner, Arnaud, Bardor, Muriel, Burel, Carole, Vauzeilles, Boris, Lerouxel, Olivier, Anderson, Charles T., Mollet, Jean-Claude, & Lerouge, Patrice. Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy- l -fucose induces defects in root cell elongation. United States. https://doi.org/10.1111/tpj.13071
Dumont, Marie, Lehner, Arnaud, Bardor, Muriel, Burel, Carole, Vauzeilles, Boris, Lerouxel, Olivier, Anderson, Charles T., Mollet, Jean-Claude, and Lerouge, Patrice. 2015.
"Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy- l -fucose induces defects in root cell elongation". United States. https://doi.org/10.1111/tpj.13071.
@article{osti_1370829,
title = {Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy- l -fucose induces defects in root cell elongation},
author = {Dumont, Marie and Lehner, Arnaud and Bardor, Muriel and Burel, Carole and Vauzeilles, Boris and Lerouxel, Olivier and Anderson, Charles T. and Mollet, Jean-Claude and Lerouge, Patrice},
abstractNote = {Screening of commercially available fluoro monosaccharides as putative growth inhibitors in Arabidopsis thaliana revealed that 2-fluoro 2-l-fucose (2F-Fuc) reduces root growth at micromolar concentrations. The inability of 2F-Fuc to affect an Atfkgp mutant that is defective in the fucose salvage pathway indicates that 2F-Fuc must be converted to its cognate GDP nucleotide sugar in order to inhibit root growth. Chemical analysis of cell wall polysaccharides and glycoproteins demonstrated that fucosylation of xyloglucans and of N-linked glycans is fully inhibited by 10 μm 2F-Fuc in Arabidopsis seedling roots, but genetic evidence indicates that these alterations are not responsible for the inhibition of root development by 2F-Fuc. Inhibition of fucosylation of cell wall polysaccharides also affected pectic rhamnogalacturonan-II (RG-II). At low concentrations, 2F-Fuc induced a decrease in RG-II dimerization. Both RG-II dimerization and root growth were partially restored in 2F-Fuc-treated seedlings by addition of boric acid, suggesting that the growth phenotype caused by 2F-Fuc was due to a deficiency of RG-II dimerization. Closer investigation of the 2F-Fuc-induced growth phenotype demonstrated that cell division is not affected by 2F-Fuc treatments. In contrast, the inhibitor suppressed elongation of root cells and promoted the emergence of adventitious roots. This study further emphasizes the importance of RG-II in cell elongation and the utility of glycosyltransferase inhibitors as new tools for studying the functions of cell wall polysaccharides in plant development. Moreover, supplementation experiments with borate suggest that the function of boron in plants might not be restricted to RG-II cross-linking, but that it might also be a signal molecule in the cell wall integrity-sensing mechanism.},
doi = {10.1111/tpj.13071},
url = {https://www.osti.gov/biblio/1370829},
journal = {The Plant Journal},
issn = {0960-7412},
number = 6,
volume = 84,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2015},
month = {Tue Dec 01 00:00:00 EST 2015}
}
Works referenced in this record:
The walls have ears: the role of plant CrRLK1Ls in sensing and transducing extracellular signals
journal, January 2011
- Boisson-Dernier, A.; Kessler, S. A.; Grossniklaus, U.
- Journal of Experimental Botany, Vol. 62, Issue 5
Novel application of 2-[18F]fluoro-2-deoxy-d-glucose to study plant defenses
journal, November 2012
- Ferrieri, Abigail P.; Appel, Heidi; Ferrieri, Richard A.
- Nuclear Medicine and Biology, Vol. 39, Issue 8
The protein N-glycosylation in plants
journal, September 1998
- Rayon, C.; Lerouge, P.; Faye, L.
- Journal of Experimental Botany, Vol. 49, Issue 326
The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1
journal, February 2002
- Vanzin, G. F.; Madson, M.; Carpita, N. C.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 5
Molecular Basis of Ca 2+ -Induced Gelation in Alginates and Pectins: The Egg-Box Model Revisited
journal, December 2001
- Braccini, Isabelle; Pérez, Serge
- Biomacromolecules, Vol. 2, Issue 4
Silencing of the GDP-d-mannose 3,5-Epimerase Affects the Structure and Cross-linking of the Pectic Polysaccharide Rhamnogalacturonan II and Plant Growth in Tomato
journal, January 2011
- Voxeur, Aline; Gilbert, Louise; Rihouey, Christophe
- Journal of Biological Chemistry, Vol. 286, Issue 10
An update on receptor-like kinase involvement in the maintenance of plant cell wall integrity
journal, April 2014
- Engelsdorf, Timo; Hamann, Thorsten
- Annals of Botany, Vol. 114, Issue 6
PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination
journal, December 2014
- Leroux, Christelle; Bouton, Sophie; Kiefer-Meyer, Marie-Christine
- Plant Physiology, Vol. 167, Issue 2
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana
journal, August 2007
- Li, Yunhai; Smith, Caroline; Corke, Fiona
- The Plant Cell, Vol. 19, Issue 8
Inhibition of β-1,4-Glucan Biosynthesis by Deoxyglucose: The Effect on the Glucosylation of Lipid Intermediates
journal, January 1983
- Datema, Roelf; Schwarz, Ralph T.; Rivas, Liliana A.
- Plant Physiology, Vol. 71, Issue 1
Rapid Structural Phenotyping of Plant Cell Wall Mutants by Enzymatic Oligosaccharide Fingerprinting
journal, December 2002
- Lerouxel, Olivier; Choo, Tze Siang; Séveno, Martial
- Plant Physiology, Vol. 130, Issue 4
Occurrence of the Primary Cell Wall Polysaccharide Rhamnogalacturonan II in Pteridophytes, Lycophytes, and Bryophytes. Implications for the Evolution of Vascular Plants
journal, December 2003
- Matsunaga, Toshiro; Ishii, Tadashi; Matsumoto, Sadamu
- Plant Physiology, Vol. 134, Issue 1
The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose
journal, March 1997
- Bonin, C. P.; Potter, I.; Vanzin, G. F.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 5
In Vitro Grown Pollen Tubes of Nicotiana alata Actively Synthesise a Fucosylated Xyloglucan
journal, October 2013
- Lampugnani, Edwin R.; Moller, Isabel E.; Cassin, Andrew
- PLoS ONE, Vol. 8, Issue 10
Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis
journal, October 2013
- Liang, Yan; Basu, Debarati; Pattathil, Sivakumar
- Journal of Experimental Botany, Vol. 64, Issue 18
Arabidopsis thaliana RGXT1 and RGXT2 Encode Golgi-Localized (1,3)-α-d-Xylosyltransferases Involved in the Synthesis of Pectic Rhamnogalacturonan-II
journal, October 2006
- Egelund, Jack; Petersen, Bent Larsen; Motawia, Mohammed Saddik
- The Plant Cell, Vol. 18, Issue 10
2-Fluoro-L-Fucose Is a Metabolically Incorporated Inhibitor of Plant Cell Wall Polysaccharide Fucosylation
journal, September 2015
- Villalobos, Jose A.; Yi, Bo R.; Wallace, Ian S.
- PLOS ONE, Vol. 10, Issue 9
Structural analysis of xyloglucans in the primary cell walls of plants in the subclass Asteridae
journal, August 2005
- Hoffman, Matt; Jia, Zhonghua; Peña, Maria J.
- Carbohydrate Research, Vol. 340, Issue 11
l-Galactose replaces l-fucose in the pectic polysaccharide rhamnogalacturonan II synthesized by the l-fucose-deficient mur1 Arabidopsis mutant
journal, February 2004
- Reuhs, Bradley L.; Glenn, Joshua; Stephens, Samuel B.
- Planta, Vol. 219, Issue 1
Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan
journal, November 2014
- Dardelle, Flavien; Le Mauff, François; Lehner, Arnaud
- Annals of Botany, Vol. 115, Issue 1
Substitution of L-Fucose by L-Galactose in Cell Walls of Arabidopsis mur1
journal, June 1996
- Zablackis, E.; York, W. S.; Pauly, M.
- Science, Vol. 272, Issue 5269
MALE GAMETOPHYTE DEFECTIVE 4 encodes a rhamnogalacturonan II xylosyltransferase and is important for growth of pollen tubes and roots in Arabidopsis: MGP4 is required for pollen tube growth
journal, December 2010
- Liu, Xiao-Lei; Liu, Lifeng; Niu, Qian-Kun
- The Plant Journal, Vol. 65, Issue 4
Two Chains of Rhamnogalacturonan II Are Cross-Linked by Borate-Diol Ester Bonds in Higher Plant Cell Walls
journal, March 1996
- Kobayashi, M.; Matoh, T.; Azuma, Ji.
- Plant Physiology, Vol. 110, Issue 3
RHAMNOGALACTURONAN II: Structure and Function of a Borate Cross-Linked Cell Wall Pectic Polysaccharide
journal, June 2004
- O'Neill, Malcolm A.; Ishii, Tadashi; Albersheim, Peter
- Annual Review of Plant Biology, Vol. 55, Issue 1
Characterization of N- Glycans from Arabidopsis. Application to a Fucose-Deficient Mutant
journal, February 1999
- Rayon, Catherine; Cabanes-Macheteau, Marion; Loutelier-Bourhis, Corinne
- Plant Physiology, Vol. 119, Issue 2
Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase
journal, August 2008
- Egelund, Jack; Damager, Iben; Faber, Kirsten
- FEBS Letters, Vol. 582, Issue 21-22
The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots
journal, January 2005
- Blilou, Ikram; Xu, Jian; Wildwater, Marjolein
- Nature, Vol. 433, Issue 7021
Rhamnogalacturonan-II, a Pectic Polysaccharide in the Walls of Growing Plant Cell, Forms a Dimer That Is Covalently Cross-linked by a Borate Ester
journal, September 1996
- O'Neill, Malcolm A.; Warrenfeltz, Dennis; Kates, Keith
- Journal of Biological Chemistry, Vol. 271, Issue 37
Fluorosugar Chain Termination Agents as Probes of the Sequence Specificity of a Carbohydrate Polymerase
journal, April 2012
- Brown, Christopher D.; Rusek, Max S.; Kiessling, Laura L.
- Journal of the American Chemical Society, Vol. 134, Issue 15
The cell wall pectic polymer rhamnogalacturonan-II is required for proper pollen tube elongation: implications of a putative sialyltransferase-like protein
journal, May 2014
- Dumont, Marie; Lehner, Arnaud; Bouton, Sophie
- Annals of Botany, Vol. 114, Issue 6
A Bifunctional Enzyme with L-Fucokinase and GDP-L-fucose Pyrophosphorylase Activities Salvages Free L-Fucose in Arabidopsis
journal, January 2008
- Kotake, Toshihisa; Hojo, Sachiko; Tajima, Noriaki
- Journal of Biological Chemistry, Vol. 283, Issue 13
Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II
journal, June 2014
- Voxeur, Aline; Fry, Stephen C.
- The Plant Journal, Vol. 79, Issue 1
Chemo-enzymatic synthesis of fluorinated sugar nucleotide: useful mechanistic Probes for glycosyltransferases
journal, August 2000
- Burkart, Michael D.; Vincent, Stéphane P.; Düffels, Arno
- Bioorganic & Medicinal Chemistry, Vol. 8, Issue 8
Primary structure of the 2-O-methyl-α-l-fucose-containing side chain of the pectic polysaccharide, rhamnogalacturonan II
journal, February 2003
- Glushka, John N.; Terrell, Mark; York, William S.
- Carbohydrate Research, Vol. 338, Issue 4
QUASIMODO1 Encodes a Putative Membrane-Bound Glycosyltransferase Required for Normal Pectin Synthesis and Cell Adhesion in Arabidopsis
journal, September 2002
- Bouton, Sophie; Leboeuf, Edouard; Mouille, Gregory
- The Plant Cell, Vol. 14, Issue 10
Callose deposition during gravitropism ofZea mays andPisum sativum and its inhibition by 2-deoxy-D-glucose
journal, January 1984
- Jaffe, M. J.; Leopold, A. C.
- Planta, Vol. 161, Issue 1
Structural Characterization of Arabidopsis Leaf Arabinogalactan Polysaccharides
journal, August 2012
- Tryfona, Theodora; Liang, Hui-Chung; Kotake, Toshihisa
- Plant Physiology, Vol. 160, Issue 2
An unambiguous nomenclature for xyloglucan-derived oligosaccharides
journal, September 1993
- Fry, Stephen C.; York, William S.; Albersheim, Peter
- Physiologia Plantarum, Vol. 89, Issue 1
The structures of arabinoxyloglucans produced by solanaceous plants
journal, May 1996
- York, William S.; Kumar Kolli, V. S.; Orlando, Ron
- Carbohydrate Research, Vol. 285
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component
journal, June 2008
- Cavalier, David M.; Lerouxel, Olivier; Neumetzler, Lutz
- The Plant Cell, Vol. 20, Issue 6
Structural identification of a bacterial quorum-sensing signal containing boron
journal, January 2002
- Chen, Xin; Schauder, Stephan; Potier, Noelle
- Nature, Vol. 415, Issue 6871
Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis
journal, June 1999
- Perrin, R. M.
- Science, Vol. 284, Issue 5422
The synthesis of the rhamnogalacturonan II component 3-deoxy-D-manno-2-octulosonic acid (Kdo) is required for pollen tube growth and elongation
journal, July 2008
- Delmas, F.; Seveno, M.; Northey, J. G. B.
- Journal of Experimental Botany, Vol. 59, Issue 10
Homogalacturonan Methyl-Esterification and Plant Development
journal, September 2009
- Wolf, Sebastian; Mouille, Grégory; Pelloux, Jérome
- Molecular Plant, Vol. 2, Issue 5
Boron deficiency results in early repression of a cytokinin receptor gene and abnormal cell differentiation in the apical root meristem of Arabidopsis thaliana
journal, April 2014
- Abreu, Isidro; Poza, Laura; Bonilla, Ildefonso
- Plant Physiology and Biochemistry, Vol. 77
Synthesis of borate cross-linked rhamnogalacturonan II
journal, April 2015
- Funakawa, Hiroya; Miwa, Kyoko
- Frontiers in Plant Science, Vol. 6
Characterization of the Cell-Wall Polysaccharides of Arabidopsis thaliana Leaves
journal, April 1995
- Zablackis, E.; Huang, J.; Muller, B.
- Plant Physiology, Vol. 107, Issue 4
Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system
journal, February 2008
- Hattori, Etsuko; Uchida, Hiroshi; Harada, Norihiro
- Planta, Vol. 227, Issue 5
Identification of Putative Rhamnogalacturonan-II Specific Glycosyltransferases in Arabidopsis Using a Combination of Bioinformatics Approaches
journal, December 2012
- Voxeur, Aline; André, Aurélie; Breton, Christelle
- PLoS ONE, Vol. 7, Issue 12
The Pore Size of Non-Graminaceous Plant Cell Walls Is Rapidly Decreased by Borate Ester Cross-Linking of the Pectic Polysaccharide Rhamnogalacturonan II
journal, November 1999
- Fleischer, Axel; O'Neill, Malcolm A.; Ehwald, Rudolf
- Plant Physiology, Vol. 121, Issue 3
MALE GAMETOPHYTE DEFECTIVE 2, Encoding a Sialyltransferase-like Protein, is Required for Normal Pollen Germination and Pollen Tube Growth in Arabidopsis
journal, August 2010
- Deng, Yi; Wang, Wei; Li, Wen-Qing
- Journal of Integrative Plant Biology, Vol. 52, Issue 9
Analysis of Structural Components and Molecular Construction of Soybean Soluble Polysaccharides by Stepwise Enzymatic Degradation
journal, January 2001
- Nakamura, Akihiro; Furuta, Hitoshi; Maeda, Hirokazu
- Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 10
Analysis of Xyloglucan Fucosylation in Arabidopsis
journal, April 2003
- Perrin, Robyn M.; Jia, Zhonghua; Wagner, Tanya A.
- Plant Physiology, Vol. 132, Issue 2
Requirement of Borate Cross-Linking of Cell Wall Rhamnogalacturonan II for Arabidopsis Growth
journal, October 2001
- O'Neill, M. A.
- Science, Vol. 294, Issue 5543
Metabolic click-labeling with a fucose analog reveals pectin delivery, architecture, and dynamics in Arabidopsis cell walls
journal, January 2012
- Anderson, C. T.; Wallace, I. S.; Somerville, C. R.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 4
Sentinels at the wall: cell wall receptors and sensors
journal, October 2007
- Humphrey, Tania V.; Bonetta, Dario T.; Goring, Daphne R.
- New Phytologist, Vol. 176, Issue 1
Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome
journal, June 2012
- Rillahan, Cory D.; Antonopoulos, Aristotelis; Lefort, Craig T.
- Nature Chemical Biology, Vol. 8, Issue 7
Auxin Controls Arabidopsis Adventitious Root Initiation by Regulating Jasmonic Acid Homeostasis
journal, June 2012
- Gutierrez, Laurent; Mongelard, Gaëlle; Floková, Kristýna
- The Plant Cell, Vol. 24, Issue 6
A fluoro analogue of UDP-α-d-glucuronic acid is an inhibitor of UDP-α-d-apiose/UDP-α-d-xylose synthase
journal, January 2011
- Choi, Sei-hyun; Ruszczycky, Mark W.; Zhang, Hua
- Chemical Communications, Vol. 47, Issue 36
Generation of Arabidopsis thaliana plants with complex N -glycans lacking β1,2-linked xylose and core α1,3-linked fucose
journal, February 2004
- Strasser, R.; Altmann, F.; Mach, L.
- FEBS Letters, Vol. 561, Issue 1-3
Development of orally active inhibitors of protein and cellular fucosylation
journal, March 2013
- Okeley, N. M.; Alley, S. C.; Anderson, M. E.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 14
Isolation of a Mutant Arabidopsis Plant That Lacks N-Acetyl Glucosaminyl Transferase I and Is Unable to Synthesize Golgi-Modified Complex N-Linked Glycans
journal, August 1993
- von Schaewen, A.; Sturm, A.; O'Neill, J.
- Plant Physiology, Vol. 102, Issue 4
2β-Deoxy-Kdo Is an Inhibitor of the Arabidopsis thaliana CMP-2-Keto-3-Deoxymanno-Octulosonic Acid Synthetase, the Enzyme Required for Activation of Kdo Prior to Incorporation into Rhamnogalacturonan II
journal, May 2013
- Smyth, Kevin M.; Mikolajek, Halina; Werner, Jörn M.
- Molecular Plant, Vol. 6, Issue 3
Real-Time Imaging of Cellulose Reorientation during Cell Wall Expansion in Arabidopsis Roots
journal, December 2009
- Anderson, Charles T.; Carroll, Andrew; Akhmetova, Laila
- Plant Physiology, Vol. 152, Issue 2
Depletion of UDP-d-apiose/UDP-d-xylose Synthases Results in Rhamnogalacturonan-II Deficiency, Cell Wall Thickening, and Cell Death in Higher Plants
journal, March 2006
- Ahn, Joon-Woo; Verma, Rajeev; Kim, Moonil
- Journal of Biological Chemistry, Vol. 281, Issue 19
Inhibitors of protein glycosylation
journal, March 1980
- Schwarz, Ralph T.; Datema, Roelf
- Trends in Biochemical Sciences, Vol. 5, Issue 3
Affinity Purification of Antibodies Specific for Asn-Linked Glycans Containing α1 → 3 Fucose or β1 → 2 Xylose
journal, February 1993
- Faye, L.; Gomord, V.; Fitchettelaine, A. C.
- Analytical Biochemistry, Vol. 209, Issue 1
Novel receptor kinases involved in growth regulation
journal, June 2008
- Hematy, K.; Hofte, H.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Membrane-Associated, Boron-Interacting Proteins Isolated by Boronate Affinity Chromatography
journal, May 2009
- Wimmer, M. A.; Lochnit, G.; Bassil, E.
- Plant and Cell Physiology, Vol. 50, Issue 7
Boron bridging of rhamnogalacturonan-II, monitored by gel electrophoresis, occurs during polysaccharide synthesis and secretion but not post-secretion
journal, January 2014
- Chormova, Dimitra; Messenger, David J.; Fry, Stephen C.
- The Plant Journal, Vol. 77, Issue 4
The Synthesis and Origin of the Pectic Polysaccharide Rhamnogalacturonan II – Insights from Nucleotide Sugar Formation and Diversity
journal, January 2012
- Bar-Peled, Maor; Urbanowicz, Breeanna R.; O’Neill, Malcolm A.
- Frontiers in Plant Science, Vol. 3
Rhamnogalacturonan II structure shows variation in the side chains monosaccharide composition and methylation status within and across different plant species
journal, July 2013
- Pabst, Martin; Fischl, Richard M.; Brecker, Lothar
- The Plant Journal