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Title: Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.

Abstract

When CO 2 is sequestered into the deep subsurface, changes to the subsurface microbial community will occur. Capnophiles, microorganisms that grow in CO 2-rich environments, are some organisms that may be selected for under the new environmental conditions. To determine whether capnophiles comprise an important part of CO 2-rich environments, an isolate from Crystal Geyser, Utah, U.S.A., a CO 2- rich spring considered a carbon sequestration analog, was characterized. The isolate was cultured under varying CO 2, pH, salinity, and temperature, as well as different carbon substrates and terminal electron acceptors (TEAs) to elucidate growth conditions and metabolic activity. Designated CG-1, the isolate is related (99%) to Lactobacillus casei in 16S rRNA gene identity, growing at PCO 2 between 0 and 1.0 MPa. Growth is inhibited at 2.5 MPa, but stationary phase cultures exposed to this pressure survive beyond 5 days. At 5.0 MPa, survival is at least 24 h. CG-1 grows in neutral pH, 0.25 M NaCl, and between 25° and 45°C and consumes glucose, lactose, sucrose, or crude oil, likely performing lactic acid fermentation. Fatty acid profiles between 0.1 and 1.0 MPa suggests decreases in cell size and increases in membrane rigidity. Transmission electron microscopy reveals rod shapedmore » bacteria at 0.1 MPa. At 1.0 MPa, cells are smaller, amorphous, and produce abundant capsular material. Its ability to grow in environments regardless of the presence of CO 2 suggests we have isolated an organism that is more capnotolerant than capnophilic. Results also show that microorganisms are capable of surviving the stressful conditions created by the introduction of CO 2 for sequestration. Furthermore, our ability to culture an environmental isolate indicates that organisms found in CO 2 environments from previous genomic and metagenomics studies are viable, metabolizing, and potentially affecting the surrounding environment.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Frontiers of Subsurface Energy Security (CFSES)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1370720
DOE Contract Number:  
SC0001114
Resource Type:
Journal Article
Journal Name:
Frontiers in Earth Science
Additional Journal Information:
Journal Volume: 3; Related Information: CFSES partners with University of Texas at Austin (lead); Sandia National Laboratory; Journal ID: ISSN 2296-6463
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; nuclear (including radiation effects), carbon sequestration

Citation Formats

Santillan, Eugenio-Felipe U., Shanahan, Timothy M., Omelon, Christopher R., Major, Jonathan R., and Bennett, Philip C. Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.. United States: N. p., 2015. Web. doi:10.3389/feart.2015.00041.
Santillan, Eugenio-Felipe U., Shanahan, Timothy M., Omelon, Christopher R., Major, Jonathan R., & Bennett, Philip C. Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.. United States. doi:10.3389/feart.2015.00041.
Santillan, Eugenio-Felipe U., Shanahan, Timothy M., Omelon, Christopher R., Major, Jonathan R., and Bennett, Philip C. Thu . "Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.". United States. doi:10.3389/feart.2015.00041.
@article{osti_1370720,
title = {Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.},
author = {Santillan, Eugenio-Felipe U. and Shanahan, Timothy M. and Omelon, Christopher R. and Major, Jonathan R. and Bennett, Philip C.},
abstractNote = {When CO2 is sequestered into the deep subsurface, changes to the subsurface microbial community will occur. Capnophiles, microorganisms that grow in CO2-rich environments, are some organisms that may be selected for under the new environmental conditions. To determine whether capnophiles comprise an important part of CO2-rich environments, an isolate from Crystal Geyser, Utah, U.S.A., a CO2- rich spring considered a carbon sequestration analog, was characterized. The isolate was cultured under varying CO2, pH, salinity, and temperature, as well as different carbon substrates and terminal electron acceptors (TEAs) to elucidate growth conditions and metabolic activity. Designated CG-1, the isolate is related (99%) to Lactobacillus casei in 16S rRNA gene identity, growing at PCO2 between 0 and 1.0 MPa. Growth is inhibited at 2.5 MPa, but stationary phase cultures exposed to this pressure survive beyond 5 days. At 5.0 MPa, survival is at least 24 h. CG-1 grows in neutral pH, 0.25 M NaCl, and between 25° and 45°C and consumes glucose, lactose, sucrose, or crude oil, likely performing lactic acid fermentation. Fatty acid profiles between 0.1 and 1.0 MPa suggests decreases in cell size and increases in membrane rigidity. Transmission electron microscopy reveals rod shaped bacteria at 0.1 MPa. At 1.0 MPa, cells are smaller, amorphous, and produce abundant capsular material. Its ability to grow in environments regardless of the presence of CO2 suggests we have isolated an organism that is more capnotolerant than capnophilic. Results also show that microorganisms are capable of surviving the stressful conditions created by the introduction of CO2 for sequestration. Furthermore, our ability to culture an environmental isolate indicates that organisms found in CO2 environments from previous genomic and metagenomics studies are viable, metabolizing, and potentially affecting the surrounding environment.},
doi = {10.3389/feart.2015.00041},
journal = {Frontiers in Earth Science},
issn = {2296-6463},
number = ,
volume = 3,
place = {United States},
year = {2015},
month = {7}
}

Works referenced in this record:

Mineral Influence on Microbial Survival During Carbon Sequestration
journal, August 2013


Multiple sequence alignment with the Clustal series of programs
journal, July 2003


Inactivation of Geobacillus stearothermophilus Spores by High-Pressure Carbon Dioxide Treatment
journal, December 2003


Effect of carbon dioxide pressure on Listeria monocytogenes in physiological saline and foods
journal, December 2000


Clustal W and Clustal X version 2.0
journal, September 2007


Microbial Community Analysis of Opalinus Clay Drill Core Samples from the Mont Terri Underground Research Laboratory, Switzerland
journal, January 2007


Observations pertaining to the origin and ecology of microorganisms recovered from the deep subsurface of Taylorsville Basin, Virginia
journal, October 1998


Resilience of planktonic and biofilm cultures to supercritical CO2
journal, December 2008

  • Mitchell, Andrew C.; Phillips, Adrienne J.; Hamilton, Marty A.
  • The Journal of Supercritical Fluids, Vol. 47, Issue 2
  • DOI: 10.1016/j.supflu.2008.07.005

Biocorrosive Thermophilic Microbial Communities in Alaskan North Slope Oil Facilities
journal, October 2009

  • Duncan, Kathleen E.; Gieg, Lisa M.; Parisi, Victoria A.
  • Environmental Science & Technology, Vol. 43, Issue 20
  • DOI: 10.1021/es9013932

Potential Impacts of Leakage from Deep CO 2 Geosequestration on Overlying Freshwater Aquifers
journal, December 2010

  • Little, Mark G.; Jackson, Robert B.
  • Environmental Science & Technology, Vol. 44, Issue 23
  • DOI: 10.1021/es102235w

Antimicrobial Effects of Pressured Carbon Dioxide in a Continuous Flow System
journal, July 1998


Review of Dense Phase CO2 Technology: Microbial and Enzyme Inactivation, and Effects on Food Quality
journal, January 2006


Disruption of Microbial Cells by the Flash Discharge of High-pressure Carbon Dioxide
journal, January 1994

  • Nakamura, Kozo; Enomoto, Atsushi; Fukushima, Hideo
  • Bioscience, Biotechnology, and Biochemistry, Vol. 58, Issue 7
  • DOI: 10.1271/bbb.58.1297

Oxygen Relieves the CO2 and Acetate Dependency of Lactobacillus johnsonii NCC 533
journal, February 2013


Spray Drying, Freeze Drying, or Freezing of Three Different Lactic Acid Bacteria Species
journal, May 1997


Microbial Growth under Supercritical CO 2
journal, February 2015

  • Peet, Kyle C.; Freedman, Adam J. E.; Hernandez, Hector H.
  • Applied and Environmental Microbiology, Vol. 81, Issue 8
  • DOI: 10.1128/AEM.03162-14

Inactivation of Escherichia coli by Carbon Dioxide under Pressure
journal, July 1996


Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production
journal, December 2007

  • Song, Hyohak; Lee, Jeong Wook; Choi, Sol
  • Biotechnology and Bioengineering, Vol. 98, Issue 6, p. 1296-1304
  • DOI: 10.1002/bit.21530

The biofilm matrix
journal, August 2010

  • Flemming, Hans-Curt; Wingender, Jost
  • Nature Reviews Microbiology, Vol. 8, Issue 9
  • DOI: 10.1038/nrmicro2415

CO2-induced shift in microbial activity affects carbon trapping and water quality in anoxic bioreactors
journal, December 2013

  • Kirk, Matthew F.; Santillan, Eugenio F. U.; Sanford, Robert A.
  • Geochimica et Cosmochimica Acta, Vol. 122
  • DOI: 10.1016/j.gca.2013.08.018

Isolation of Bacteria Whose Growth Is Dependent on High Levels of CO2 and Implications of Their Potential Diversity
journal, May 2008

  • Ueda, K.; Tagami, Y.; Kamihara, Y.
  • Applied and Environmental Microbiology, Vol. 74, Issue 14
  • DOI: 10.1128/AEM.00491-08

Cell Size Control in Bacteria
journal, May 2012


Metagenomic analysis of a high carbon dioxide subsurface microbial community populated by chemolithoautotrophs and bacteria and archaea from candidate phyla: High CO
journal, April 2015

  • Emerson, Joanne B.; Thomas, Brian C.; Alvarez, Walter
  • Environmental Microbiology, Vol. 18, Issue 6
  • DOI: 10.1111/1462-2920.12817

Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract
journal, September 2006


The influence of pressure and temperature of compressed CO2 on the survival of yeast cells
journal, May 1995


Escherichia coli inactivation mechanism by pressurized CO 2
journal, December 2006

  • Oulé, Mathias K.; Tano, Kablan; Bernier, Anne-Marie
  • Canadian Journal of Microbiology, Vol. 52, Issue 12
  • DOI: 10.1139/w06-078

The impact of SO2 on post combustion carbon dioxide capture in bed of silica sand through hydrate formation
journal, July 2013

  • Daraboina, Nagu; Ripmeester, John; Englezos, Peter
  • International Journal of Greenhouse Gas Control, Vol. 15
  • DOI: 10.1016/j.ijggc.2013.02.008

Man-made versus natural CO2 leakage: A 400 k.y. history of an analogue for engineered geological storage of CO2
journal, April 2013

  • Burnside, Neil M.; Shipton, Zoe K.; Dockrill, Ben
  • Geology, Vol. 41, Issue 4
  • DOI: 10.1130/g33738.1

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH
journal, September 2003


Microbial Activity at Gigapascal Pressures
journal, February 2002


Prokaryotic carbonic anhydrases
journal, October 2000


Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli
journal, July 1999


Non-thermal bacterial inactivation with dense CO2
journal, October 2003

  • Spilimbergo, S.; Bertucco, A.
  • Biotechnology and Bioengineering, Vol. 84, Issue 6
  • DOI: 10.1002/bit.10783

Inactivation Kinetics of Lactobacillus plantarum by High Pressure Carbon Dioxide
journal, July 1999


Bacterial Calcium Carbonate Precipitation in Cave Environments: A Function of Calcium Homeostasis
journal, June 2010

  • Banks, Eric D.; Taylor, Nicholas M.; Gulley, Jason
  • Geomicrobiology Journal, Vol. 27, Issue 5
  • DOI: 10.1080/01490450903485136

A Method for the Production of Pre-reduced Anaerobically Sterilized Culture Media
journal, February 1977


Monitoring of the microbial community composition in deep subsurface saline aquifers during CO2 storage in Ketzin, Germany
journal, January 2011


The relevance of carbon dioxide metabolism in Streptococcus thermophilus
journal, April 2009


Acid habituation of Escherichia coli and the potential role of cyclopropane fatty acids in low pH tolerance
journal, July 1997

  • Brown, Janelle L.; Ross, Thomas; McMeekin, Thomas A.
  • International Journal of Food Microbiology, Vol. 37, Issue 2-3
  • DOI: 10.1016/S0168-1605(97)00068-8

Changes in the deep subsurface microbial biosphere resulting from a field-scale CO2 geosequestration experiment
journal, May 2014


Depth-Resolved Quantification of Anaerobic Toluene Degraders and Aquifer Microbial Community Patterns in Distinct Redox Zones of a Tar Oil Contaminant Plume
journal, December 2007

  • Winderl, C.; Anneser, B.; Griebler, C.
  • Applied and Environmental Microbiology, Vol. 74, Issue 3
  • DOI: 10.1128/AEM.01951-07

Alteration in cellular fatty acid composition as a response to salt, acid, oxidative and thermal stresses in Lactobacillus helveticus
journal, August 2001