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Title: Microbial Growth under Supercritical CO 2

Abstract

Growth of microorganisms in environments containing CO 2above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. Thus, supercritical CO 2(scCO 2) is generally regarded as a sterilizing agent. We report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (GCS) that are capable of growth in pressurized bioreactors containing scCO 2. Analysis of 16S rRNA genes from scCO 2enrichment cultures revealed microbial assemblages of varied complexity, including representatives of the genusBacillus. Propagation of enrichment cultures under scCO 2headspace led to isolation of six strains corresponding toBacillus cereus,Bacillus subterraneus,Bacillus amyloliquefaciens,Bacillus safensis, andBacillus megaterium. Isolates are spore-forming, facultative anaerobes and capable of germination and growth under an scCO 2headspace. In addition to these isolates, severalBacillustype strains grew under scCO 2, suggesting that this may be a shared feature of spore-formingBacillusspp. Our results provide direct evidence of microbial activity at the interface between scCO 2and an aqueous phase. Since microbial activity can influence the key mechanisms for permanent storage of sequestered CO 2(i.e., structural, residual, solubility, and mineral trapping), our work suggests that during GCS microorganisms may grow and catalyze biological reactions that influence the fate and transport of COmore » 2in the deep subsurface.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Nanoscale Control of Geologic CO2 (NCGC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1370615
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 81; Journal Issue: 8; Related Information: NCGC partners with Lawrence Berkeley National Laboratory (lead); University of California, Davis; Lawrence Livermore National Laboratory; Massachusetts Institute of Technology; Ohio State University; Oak Ridge National Laboratory; Washington University, St. Louis
Country of Publication:
United States
Language:
English
Subject:
bio-inspired, mechanical behavior, carbon sequestration

Citation Formats

Peet, Kyle C., Freedman, Adam J. E., Hernandez, Hector H., Britto, Vanya, Boreham, Chris, Ajo-Franklin, Jonathan B., Thompson, Janelle R., and Nojiri, H. Microbial Growth under Supercritical CO 2. United States: N. p., 2015. Web. doi:10.1128/AEM.03162-14.
Peet, Kyle C., Freedman, Adam J. E., Hernandez, Hector H., Britto, Vanya, Boreham, Chris, Ajo-Franklin, Jonathan B., Thompson, Janelle R., & Nojiri, H. Microbial Growth under Supercritical CO 2. United States. doi:10.1128/AEM.03162-14.
Peet, Kyle C., Freedman, Adam J. E., Hernandez, Hector H., Britto, Vanya, Boreham, Chris, Ajo-Franklin, Jonathan B., Thompson, Janelle R., and Nojiri, H. Fri . "Microbial Growth under Supercritical CO 2". United States. doi:10.1128/AEM.03162-14.
@article{osti_1370615,
title = {Microbial Growth under Supercritical CO 2},
author = {Peet, Kyle C. and Freedman, Adam J. E. and Hernandez, Hector H. and Britto, Vanya and Boreham, Chris and Ajo-Franklin, Jonathan B. and Thompson, Janelle R. and Nojiri, H.},
abstractNote = {Growth of microorganisms in environments containing CO2above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. Thus, supercritical CO2(scCO2) is generally regarded as a sterilizing agent. We report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (GCS) that are capable of growth in pressurized bioreactors containing scCO2. Analysis of 16S rRNA genes from scCO2enrichment cultures revealed microbial assemblages of varied complexity, including representatives of the genusBacillus. Propagation of enrichment cultures under scCO2headspace led to isolation of six strains corresponding toBacillus cereus,Bacillus subterraneus,Bacillus amyloliquefaciens,Bacillus safensis, andBacillus megaterium. Isolates are spore-forming, facultative anaerobes and capable of germination and growth under an scCO2headspace. In addition to these isolates, severalBacillustype strains grew under scCO2, suggesting that this may be a shared feature of spore-formingBacillusspp. Our results provide direct evidence of microbial activity at the interface between scCO2and an aqueous phase. Since microbial activity can influence the key mechanisms for permanent storage of sequestered CO2(i.e., structural, residual, solubility, and mineral trapping), our work suggests that during GCS microorganisms may grow and catalyze biological reactions that influence the fate and transport of CO2in the deep subsurface.},
doi = {10.1128/AEM.03162-14},
journal = {Applied and Environmental Microbiology},
number = 8,
volume = 81,
place = {United States},
year = {Fri Feb 13 00:00:00 EST 2015},
month = {Fri Feb 13 00:00:00 EST 2015}
}