Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Langford, Seth T.; Wiggins, Cody S.; Santos, Roque; Hauser, Melinda; Becker, Jeffrey M.; Ruggles, Arthur E.; Chen, ed., Chin-Tu

doi:10.1371/journal.pone.0180503

Title: Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Journal Article · Thu Jul 06 00:00:00 EDT 2017 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0180503· OSTI ID:1368434

Langford, Seth T.;

; Santos, Roque; Hauser, Melinda; Becker, Jeffrey M.; Ruggles, Arthur E.; Chen, ed., Chin-Tu

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by ¹⁸F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.

View Journal Article

Cite

Export

Save

Research Organization:: Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)

Grant/Contract Number:: NA0001983; GM112496

OSTI ID:: 1368434

Alternate ID(s):: OSTI ID: 1393145

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 12 Journal Issue: 7; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 7 works

Citation information provided by
Web of Science

References (26)

Assessment of the Tissue Distribution of Transplanted Human Endothelial Progenitor Cells by Radioactive Labeling Aicher, Alexandra; Brenner, Winfried; Zuhayra, Maaz Circulation, Vol. 107, Issue 16 https://doi.org/10.1161/01.CIR.0000062649.63838.C9	journal	April 2003
Multiple-particle tracking—an improvement for positron particle tracking Yang, Z.; Parker, D. J.; Fryer, P. J. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 564, Issue 1 https://doi.org/10.1016/j.nima.2006.04.054	journal	August 2006
Evaluation of a BGO-Based PET System for Single-Cell Tracking Performance by Simulation and Phantom Studies Ouyang, Yu; Kim, Tae Jin; Pratx, Guillem Molecular Imaging, Vol. 15 https://doi.org/10.1177/1536012116646489	journal	January 2016
Image reconstruction for PET/CT scanners: past achievements and future challenges Tong, Shan; Alessio, Adam M.; Kinahan, Paul E. Imaging in Medicine, Vol. 2, Issue 5 https://doi.org/10.2217/iim.10.49	journal	October 2010
Noninvasive cell-tracking methods Kircher, Moritz F.; Gambhir, Sanjiv S.; Grimm, Jan Nature Reviews Clinical Oncology, Vol. 8, Issue 11 https://doi.org/10.1038/nrclinonc.2011.141	journal	September 2011
GATE: a simulation toolkit for PET and SPECT Jan, S.; Santin, G.; Strul, D. Physics in Medicine and Biology, Vol. 49, Issue 19 https://doi.org/10.1088/0031-9155/49/19/007	journal	September 2004
A simple method for stem cell labeling with fluorine 18 Ma, Bing; Hankenson, Kurt D.; Dennis, James E. Nuclear Medicine and Biology, Vol. 32, Issue 7 https://doi.org/10.1016/j.nucmedbio.2005.04.018	journal	October 2005
Positron emission particle tracking - a technique for studying flow within engineering equipment Parker, D. J.; Broadbent, C. J.; Fowles, P. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 326, Issue 3 https://doi.org/10.1016/0168-9002(93)90864-E	journal	March 1993
Feature point tracking and trajectory analysis for video imaging in cell biology Sbalzarini, I. F.; Koumoutsakos, P. Journal of Structural Biology, Vol. 151, Issue 2 https://doi.org/10.1016/j.jsb.2005.06.002	journal	August 2005
X-Ray Survival Characteristics and Genetic Analysis for Nine Saccharomyces Deletion Mutants That Show Altered Radiation Sensitivity Game, John C.; Williamson, Marsha S.; Baccari, Clelia Genetics, Vol. 169, Issue 1 https://doi.org/10.1534/genetics.104.028613	journal	September 2004
In vivo MRI of cancer cell fate at the single-cell level in a mouse model of breast cancer metastasis to the brain Heyn, Chris; Ronald, John A.; Ramadan, Soha S. Magnetic Resonance in Medicine, Vol. 56, Issue 5 https://doi.org/10.1002/mrm.21029	journal	November 2006
A Lagrangian study of liquid flow in a reverse-flow hydrocyclone using positron emission particle tracking Chang, Yu-Fen; Hoffmann, Alex C. Experiments in Fluids, Vol. 56, Issue 1 https://doi.org/10.1007/s00348-014-1875-5	journal	January 2015
Eukaryotic resistance to fluoride toxicity mediated by a widespread family of fluoride export proteins Li, S.; Smith, K. D.; Davis, J. H. Proceedings of the National Academy of Sciences, Vol. 110, Issue 47 https://doi.org/10.1073/pnas.1310439110	journal	October 2013
The shape and behaviour of a granular bed in a rotating drum using Eulerian flow fields obtained from PEPT Morrison, A. J.; Govender, I.; Mainza, A. N. Chemical Engineering Science, Vol. 152 https://doi.org/10.1016/j.ces.2016.06.022	journal	October 2016
Performance Evaluation of the Inveon Dedicated PET Preclinical Tomograph Based on the NEMA NU-4 Standards Bao, Q.; Newport, D.; Chen, M. Journal of Nuclear Medicine, Vol. 50, Issue 3 https://doi.org/10.2967/jnumed.108.056374	journal	February 2009
Ex vivo cell labeling with 64Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography Adonai, N.; Nguyen, K. N.; Walsh, J. Proceedings of the National Academy of Sciences, Vol. 99, Issue 5 https://doi.org/10.1073/pnas.052709599	journal	February 2002
The Use of lndium-111-Labeled Leukocytes for Abscess Detection Doherty, Paul W.; Bushberg, Jerrold T.; Lipton, Martin J. Clinical Nuclear Medicine, Vol. 3, Issue 3 https://doi.org/10.1097/00003072-197803000-00008	journal	January 1978
A method for labeling cells for positron emission tomography (PET) studies Melder, Robert J.; Elmaleh, David; Brownell, Anna Liisa Journal of Immunological Methods, Vol. 175, Issue 1 https://doi.org/10.1016/0022-1759(94)90333-6	journal	September 1994
Positron Emission Particle Tracking (PEPT) for Fluid Flow Measurements Langford, Seth; Wiggins, Cody; Tenpenny, Daniel Nuclear Engineering and Design, Vol. 302 https://doi.org/10.1016/j.nucengdes.2016.01.017	journal	June 2016
¹⁸ F-FDG Cell Labeling May Underestimate Transplanted Cell Homing: More Accurate, Efficient, and Stable Cell Labeling with Hexadecyl-4-[ ¹⁸ F]Fluorobenzoate for in Vivo Tracking of Transplanted Human Progenitor Cells by Positron Emission Tomography Zhang, Yan; Dasilva, Jean N.; Hadizad, Tayebeh Cell Transplantation, Vol. 21, Issue 9 https://doi.org/10.3727/096368911X637416	journal	September 2012
Positron Emission Particle Tracking (PEPT) for the analysis of water motion in a domestic dishwasher Pérez-Mohedano, R.; Letzelter, N.; Amador, C. Chemical Engineering Journal, Vol. 259 https://doi.org/10.1016/j.cej.2014.08.033	journal	January 2015
Single-Cell Tracking With PET Using a Novel Trajectory Reconstruction Algorithm Lee, Keum Sil; Kim, Tae Jin; Pratx, Guillem IEEE Transactions on Medical Imaging, Vol. 34, Issue 4 https://doi.org/10.1109/TMI.2014.2373351	journal	April 2015
A novel clustering approach to positron emission particle tracking Wiggins, Cody; Santos, Roque; Ruggles, Arthur Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 811 https://doi.org/10.1016/j.nima.2015.11.136	journal	March 2016
Methods of Digital Video Microscopy for Colloidal Studies Crocker, John C.; Grier, David G. Journal of Colloid and Interface Science, Vol. 179, Issue 1 https://doi.org/10.1006/jcis.1996.0217	journal	April 1996
On the Imaging of Very Weak Sources in an LSO Pet Scanner Goertzen, Andrew L.; Suk, Joon Young; Thompson, Christopher J. 2006 IEEE Nuclear Science Symposium Conference Record https://doi.org/10.1109/NSSMIC.2006.354379	conference	October 2006
Positron Emission Particle Tracking (PEPT) Validation for Jet Flow Langford, Seth; Wiggins, Cody; Patel, Nitant 2016 24th International Conference on Nuclear Engineering, Volume 5: Student Paper Competition https://doi.org/10.1115/ICONE24-60880	conference	October 2016

Similar Records

Noninvasive interrogation of local flow phenomena in twisted tape swirled flow via positron emission particle tracking ($\mathrm{PEPT}$)

Journal Article · Thu Dec 16 00:00:00 EST 2021 · Nuclear Engineering and Design · OSTI ID:1368434

Wiggins, Cody S.; Carasik, Lane B.; Ruggles, Arthur E.

Positron emission tracking of individual particles in particle-laden rimming flow

Journal Article · Thu May 15 00:00:00 EDT 2014 · Physics of Fluids (1994) · OSTI ID:1368434

Seville, J. P. K.,

Coregistered positron emission particle tracking (PEPT) and X-ray computed tomography (CT) for engineering flow measurements

Journal Article · Wed Jan 04 00:00:00 EST 2023 · Nuclear Engineering and Design · OSTI ID:1368434

Wiggins, Cody S.; Cabral, Arturo; Carasik, Lane B.

Related Subjects

60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES

Title: Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Citation Formats

References (26)

Similar Records

Related Subjects