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Title: Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Abstract

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.

Authors:
 [1]; ORCiD logo [2];  [3];  [4];  [4];  [1];  [5]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Nuclear Engineering
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Physics and Astronomy
  3. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Nuclear Engineering; Escuela Politecnica Nacional, Quito (Ecuador). Dept. de Ciencias Nucleares
  4. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
  5. Univ. of Chicago, IL (United States)
Publication Date:
Research Org.:
Univ. of Tennessee, Knoxville, TN (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
OSTI Identifier:
1368434
Alternate Identifier(s):
OSTI ID: 1393145
Grant/Contract Number:
NA0001983; GM112496
Resource Type:
Journal Article: Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., and Chen, Chin-Tu. Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0180503.
Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., & Chen, Chin-Tu. Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking. United States. doi:10.1371/journal.pone.0180503.
Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., and Chen, Chin-Tu. Thu . "Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking". United States. doi:10.1371/journal.pone.0180503.
@article{osti_1368434,
title = {Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking},
author = {Langford, Seth T. and Wiggins, Cody S. and Santos, Roque and Hauser, Melinda and Becker, Jeffrey M. and Ruggles, Arthur E. and Chen, Chin-Tu},
abstractNote = {A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.},
doi = {10.1371/journal.pone.0180503},
journal = {PLoS ONE},
number = 7,
volume = 12,
place = {United States},
year = {Thu Jul 06 00:00:00 EDT 2017},
month = {Thu Jul 06 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1371/journal.pone.0180503

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  • A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for verymore » low activity tracers.« less
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  • To evaluate the feasibility of probing the distribution of angiotensin-converting enzyme (ACE) in vivo using positron emission tomography (PET), 4-cis-(18F)fluorocaptopril (18FCAP) was prepared by the reaction of the triflate 2 with K18F/Kryptofix 222 in MeCN followed by hydrolysis (2 N NaOH). The synthesis time was 1 hr with an average radiochemical yield (EOS) of 12% and a specific activity of greater than 300 Ci/mmol. In vivo biodistribution in rats at 30 min after administration showed high uptakes into organs known to have high ACE concentration (lung, kidney and aorta) and faster clearance of 18FCAP for lung and kidney, compared tomore » the clearance from the aorta. When different amounts of unlabeled 4-cis-fluorocaptopril (SQ 25750) were coinjected in rats, a dose of greater than 5 micrograms/kg decreased the lung uptake by one-half while only 1 microgram/kg decreased the kidney uptake by one-half. In general, the binding in the four tissues studied was saturable with the expected capacity. 18FCAP was administered to a human and displaceable uptake observed in the lung and kidney. The results demonstrate the feasibility of probing ACE in vivo using PET.« less
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