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Title: Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy

Abstract

A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. Here, the CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.

Authors:
 [1];  [1];  [1];  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1367975
Report Number(s):
LLNL-JRNL-693042
Journal ID: ISSN 0003-2700
Grant/Contract Number:
AC52-07NA27344
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 88; Journal Issue: 17; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 07 ISOTOPES AND RADIATION SOURCES

Citation Formats

McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, and Turteltaub, Kenneth W. Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy. United States: N. p., 2016. Web. doi:10.1021/acs.analchem.6b02054.
McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, & Turteltaub, Kenneth W. Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy. United States. doi:10.1021/acs.analchem.6b02054.
McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, and Turteltaub, Kenneth W. Tue . "Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy". United States. doi:10.1021/acs.analchem.6b02054. https://www.osti.gov/servlets/purl/1367975.
@article{osti_1367975,
title = {Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy},
author = {McCartt, A. Daniel and Ognibene, Ted J. and Bench, Graham and Turteltaub, Kenneth W.},
abstractNote = {A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. Here, the CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.},
doi = {10.1021/acs.analchem.6b02054},
journal = {Analytical Chemistry},
number = 17,
volume = 88,
place = {United States},
year = {Tue Jul 26 00:00:00 EDT 2016},
month = {Tue Jul 26 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 1work
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