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Title: In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel

Abstract

Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.

Authors:
; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1367386
Report Number(s):
PNNL-SA-109594
Journal ID: ISSN 0003-2654; 48569; 49694
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analyst
Additional Journal Information:
Journal Volume: 142; Journal Issue: 13; Journal ID: ISSN 0003-2654
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
SALVI; microfluidics; diffusion coefficient; relaxometry; MRI; nuclear magnetic resonance; biofilm; Shewanella; Environmental Molecular Sciences Laboratory

Citation Formats

Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., and Yu, X. -Y. In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel. United States: N. p., 2017. Web. doi:10.1039/C7AN00078B.
Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., & Yu, X. -Y. In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel. United States. doi:10.1039/C7AN00078B.
Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., and Yu, X. -Y. Sun . "In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel". United States. doi:10.1039/C7AN00078B.
@article{osti_1367386,
title = {In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel},
author = {Renslow, R. S. and Marshall, M. J. and Tucker, A. E. and Chrisler, W. B. and Yu, X. -Y.},
abstractNote = {Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.},
doi = {10.1039/C7AN00078B},
journal = {Analyst},
issn = {0003-2654},
number = 13,
volume = 142,
place = {United States},
year = {2017},
month = {1}
}

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