Acquired thermotolerance and heat shock proteins in thermophiles form the three phylogenetic domains
- Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology
- Univ. of Copenhagen (Denmark). Institute of Molecular Biology
Thermophilic organisms from each of the three phylogenetic domains (Bacteria, Archaea, and Eucarya) acquired thermotolerance after heat shock. Bacillus caldolyticus grown at 60 C and heat shocked at 69 C for 10 min showed thermotolerance at 74 C, Sulfolobus shibatae grown at 70 C and heat shocked at 88 C for 60 min showed thermotolerance at 95 C, and Thermomyces lanuginosus grown at 50 C and hat shocked at 55 C for 60 min showed thermotolerance at 58 C. Determinations of protein synthesis during heat shock revealed differences in the dominant heat shock proteins for each species. For B. caldolyticus, a 70-kDa protein dominated while for S. shibatae, a 55-kDa protein dominated and for T. lanuginosus, 31- to 33-kDa proteins dominated. Reagents that disrupted normal protein synthesis during heat shock prevented the enhanced thermotolerance.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 136722
- Journal Information:
- Journal of Bacteriology, Vol. 176, Issue 19; Other Information: PBD: Oct 1994
- Country of Publication:
- United States
- Language:
- English
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