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Title: Methods of introducing nucleic acids into cellular DNA

Abstract

A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.

Inventors:
; ; ;
Publication Date:
Research Org.:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1366739
Patent Number(s):
9,688,994
Application Number:
13/954,351
Assignee:
President and Fellows of Harvard College CHO
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Jul 30
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Methods of introducing nucleic acids into cellular DNA. United States: N. p., 2017. Web.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., & Church, George M. Methods of introducing nucleic acids into cellular DNA. United States.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Tue . "Methods of introducing nucleic acids into cellular DNA". United States. doi:. https://www.osti.gov/servlets/purl/1366739.
@article{osti_1366739,
title = {Methods of introducing nucleic acids into cellular DNA},
author = {Lajoie, Marc J. and Gregg, Christopher J. and Mosberg, Joshua A. and Church, George M.},
abstractNote = {A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 27 00:00:00 EDT 2017},
month = {Tue Jun 27 00:00:00 EDT 2017}
}

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