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Title: Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass

Abstract

Elevating the lipid content in vegetative tissues has emerged as a new strategy for increasing energy density and biofuel yield of crops. Storage lipids in contrast to structural and signaling lipids are mainly composed of glycerol esters of fatty acids, also known as triacylglycerol (TAG). TAGs are one of the most energy-rich and abundant forms of reduced carbon available in nature. Therefore, altering the carbon-partitioning balance in favour of TAG in vegetative tissues of sugarcane, one of the highest yielding biomass crops, is expected to drastically increase energy yields. We report metabolic engineering to elevate TAG accumulation in vegetative tissues of sugarcane. Constitutive co-expression of WRINKLED1 (WRI1), diacylglycerol acyltransferase1-2 (DGAT1-2) and oleosin1 (OLE1) and simultaneous cosuppression of ADP-glucose pyrophosphorylase (AGPase) and a subunit of the peroxisomal ABC transporter1 (PXA1) in transgenic sugarcane elevated TAG accumulation in leaves or stems by 95- or 43-fold to 1.9% or 0.9% of dry weight (DW), respectively, while expression or suppression of one to three of the target genes increased TAG levels by 1.5- to 9.5-fold. Accumulation of TAG in vegetative progeny plants was consistent with the results from primary transgenics and contributed to a total fatty acid content of up to 4.7% or 1.7%more » of DW in mature leaves or stems, respectively. We saw lipid droplets in mesophyll cells of transgenic leaves by confocal fluorescence microscopy. These results provide the basis for optimizations of TAG accumulation in sugarcane and other high yielding biomass grasses and will open new prospects for biofuel applications.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1];  [2];  [2];  [2];  [1]
  1. Univ. of Florida, Gainesville, FL (United States). Genetics Inst.
  2. Brookhaven National Lab. (BNL), Upton, NY (United States). Biosciences Dept.
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1363860
Alternate Identifier(s):
OSTI ID: 1401627
Grant/Contract Number:  
AR0000206
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 2; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Triacylglycerol; fatty acids; vegetative oil; sugarcane; biofuel; biodiese

Citation Formats

Zale, Janice, Jung, Je Hyeong, Kim, Jae Yoon, Pathak, Bhuvan, Karan, Ratna, Liu, Hui, Chen, Xiuhua, Wu, Hao, Candreva, Jason, Zhai, Zhiyang, Shanklin, John, and Altpeter, Fredy. Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass. United States: N. p., 2015. Web. doi:10.1111/pbi.12411.
Zale, Janice, Jung, Je Hyeong, Kim, Jae Yoon, Pathak, Bhuvan, Karan, Ratna, Liu, Hui, Chen, Xiuhua, Wu, Hao, Candreva, Jason, Zhai, Zhiyang, Shanklin, John, & Altpeter, Fredy. Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass. United States. doi:10.1111/pbi.12411.
Zale, Janice, Jung, Je Hyeong, Kim, Jae Yoon, Pathak, Bhuvan, Karan, Ratna, Liu, Hui, Chen, Xiuhua, Wu, Hao, Candreva, Jason, Zhai, Zhiyang, Shanklin, John, and Altpeter, Fredy. Mon . "Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass". United States. doi:10.1111/pbi.12411. https://www.osti.gov/servlets/purl/1363860.
@article{osti_1363860,
title = {Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass},
author = {Zale, Janice and Jung, Je Hyeong and Kim, Jae Yoon and Pathak, Bhuvan and Karan, Ratna and Liu, Hui and Chen, Xiuhua and Wu, Hao and Candreva, Jason and Zhai, Zhiyang and Shanklin, John and Altpeter, Fredy},
abstractNote = {Elevating the lipid content in vegetative tissues has emerged as a new strategy for increasing energy density and biofuel yield of crops. Storage lipids in contrast to structural and signaling lipids are mainly composed of glycerol esters of fatty acids, also known as triacylglycerol (TAG). TAGs are one of the most energy-rich and abundant forms of reduced carbon available in nature. Therefore, altering the carbon-partitioning balance in favour of TAG in vegetative tissues of sugarcane, one of the highest yielding biomass crops, is expected to drastically increase energy yields. We report metabolic engineering to elevate TAG accumulation in vegetative tissues of sugarcane. Constitutive co-expression of WRINKLED1 (WRI1), diacylglycerol acyltransferase1-2 (DGAT1-2) and oleosin1 (OLE1) and simultaneous cosuppression of ADP-glucose pyrophosphorylase (AGPase) and a subunit of the peroxisomal ABC transporter1 (PXA1) in transgenic sugarcane elevated TAG accumulation in leaves or stems by 95- or 43-fold to 1.9% or 0.9% of dry weight (DW), respectively, while expression or suppression of one to three of the target genes increased TAG levels by 1.5- to 9.5-fold. Accumulation of TAG in vegetative progeny plants was consistent with the results from primary transgenics and contributed to a total fatty acid content of up to 4.7% or 1.7% of DW in mature leaves or stems, respectively. We saw lipid droplets in mesophyll cells of transgenic leaves by confocal fluorescence microscopy. These results provide the basis for optimizations of TAG accumulation in sugarcane and other high yielding biomass grasses and will open new prospects for biofuel applications.},
doi = {10.1111/pbi.12411},
journal = {Plant Biotechnology Journal},
number = 2,
volume = 14,
place = {United States},
year = {Mon Jun 08 00:00:00 EDT 2015},
month = {Mon Jun 08 00:00:00 EDT 2015}
}

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Cited by: 27 works
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Works referenced in this record:

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